##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062528_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2255887
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14036828972373	32.0	32.0	32.0	32.0	32.0
2	30.679550438474976	32.0	32.0	32.0	32.0	32.0
3	30.671516348114956	32.0	32.0	32.0	32.0	32.0
4	30.76667891609819	32.0	32.0	32.0	32.0	32.0
5	30.493221070027	32.0	32.0	32.0	32.0	32.0
6	34.16488591848794	36.0	36.0	36.0	32.0	36.0
7	34.04314356171209	36.0	36.0	36.0	32.0	36.0
8	34.02559259395528	36.0	36.0	36.0	32.0	36.0
9	34.24903064736842	36.0	36.0	36.0	32.0	36.0
10	33.80636840409116	36.0	36.0	36.0	32.0	36.0
11	34.21638317876737	36.0	36.0	36.0	32.0	36.0
12	33.93624370369615	36.0	36.0	36.0	32.0	36.0
13	34.097956590910805	36.0	36.0	36.0	32.0	36.0
14	33.97239312075472	36.0	36.0	36.0	32.0	36.0
15	33.892741524730624	36.0	36.0	36.0	32.0	36.0
16	33.89967006326115	36.0	36.0	36.0	32.0	36.0
17	33.806910097890544	36.0	36.0	36.0	32.0	36.0
18	33.82067098219015	36.0	36.0	36.0	32.0	36.0
19	33.81635339004126	36.0	36.0	36.0	32.0	36.0
20	33.8134702669061	36.0	36.0	36.0	32.0	36.0
21	33.79277552466059	36.0	36.0	36.0	32.0	36.0
22	33.73730510437801	36.0	36.0	36.0	32.0	36.0
23	33.697372253131476	36.0	36.0	36.0	27.0	36.0
24	33.656573223747465	36.0	36.0	36.0	27.0	36.0
25	33.18125774916918	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	15.0
4	102.0
5	229.0
6	329.0
7	118.0
8	343.0
9	441.0
10	240.0
11	87.0
12	170.0
13	115.0
14	342.0
15	486.0
16	710.0
17	982.0
18	1344.0
19	2025.0
20	3173.0
21	4922.0
22	7530.0
23	11574.0
24	16475.0
25	23415.0
26	31957.0
27	40485.0
28	53812.0
29	70216.0
30	89183.0
31	119956.0
32	169622.0
33	239932.0
34	506754.0
35	858803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.07202183645974	16.848334225157235	10.938445530986927	26.141198407396093
2	17.318539521093996	19.298539592022017	36.389799131841	26.99312175504298
3	19.131175679940988	23.02671709702702	27.999444982321002	29.842662240710986
4	12.857086487274946	14.73497579927564	35.01543552658355	37.39250218686586
5	15.05152289418628	36.030551005403716	33.207899911046624	15.710026189363377
6	35.40595652436147	34.52014127631192	16.30065919484565	13.773243004480964
7	31.191848666248216	29.90247764881751	20.221926005442796	18.68374767949147
8	28.739246369609873	32.49883534688915	18.914410197480798	19.84750808602017
9	27.25207047031235	14.217325615705622	18.184286833073394	40.346317080908634
10	16.05301372075973	26.125318875852777	31.065209557133144	26.75645784625435
11	38.20708974847739	20.7455894890682	21.541125787048358	19.506194975406057
12	24.75411320513515	23.27715008349365	28.22808813307138	23.74064857829982
13	29.641310416635232	18.801444735494282	25.070101981851156	26.487142866019337
14	23.813687394450746	19.15866840945455	24.331108985639755	32.696535210454954
15	25.636362426025656	26.699063379953536	21.779422761575724	25.885151432445085
16	26.57383590994378	25.353518409051773	23.027823487539433	25.04482219346501
17	24.62140579617518	25.57339490260409	24.70511372916622	25.10008557205451
18	25.652730098955935	24.35201928872815	25.478733839875506	24.516516772440415
19	25.951575216965182	24.856806528130903	24.719738505461414	24.471879749442497
20	25.91870293973401	23.94452963590084	24.556887303908624	25.579880120456526
21	27.03360697395199	23.94783739577577	23.74836603482981	25.27018959544243
22	26.034829660884512	24.019733196948387	24.296010385145404	25.649426757021693
23	24.57783009864318	23.875937403174586	24.946532396174305	26.599700102007933
24	25.00381741058825	24.841133575635332	24.489044031611712	25.666004982164704
25	25.3835460051027	24.090071391667006	24.587203119146494	25.939179484083795
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	254.0
1	254.0
2	568.5
3	883.0
4	883.0
5	883.0
6	2150.0
7	3417.0
8	3417.0
9	3417.0
10	3715.0
11	4013.0
12	4013.0
13	4013.0
14	4760.5
15	5508.0
16	5508.0
17	5508.0
18	8800.5
19	12093.0
20	12093.0
21	12093.0
22	19810.5
23	27528.0
24	27528.0
25	27528.0
26	41355.5
27	55183.0
28	55183.0
29	55183.0
30	68841.5
31	82500.0
32	82500.0
33	82500.0
34	105557.5
35	128615.0
36	128615.0
37	128615.0
38	149633.0
39	170651.0
40	170651.0
41	170651.0
42	198386.5
43	226122.0
44	226122.0
45	226122.0
46	262161.0
47	298200.0
48	298200.0
49	298200.0
50	301273.0
51	304346.0
52	304346.0
53	304346.0
54	285775.5
55	267205.0
56	267205.0
57	267205.0
58	247425.5
59	227646.0
60	227646.0
61	227646.0
62	203798.5
63	179951.0
64	179951.0
65	179951.0
66	150551.0
67	121151.0
68	121151.0
69	121151.0
70	92471.0
71	63791.0
72	63791.0
73	63791.0
74	50272.5
75	36754.0
76	36754.0
77	36754.0
78	30008.0
79	23262.0
80	23262.0
81	23262.0
82	16401.5
83	9541.0
84	9541.0
85	9541.0
86	6960.0
87	4379.0
88	4379.0
89	4379.0
90	2977.0
91	1575.0
92	1575.0
93	1575.0
94	938.5
95	302.0
96	302.0
97	302.0
98	659.5
99	1017.0
100	1017.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009308976912407403
2	0.0035019484575246895
3	0.004565831533228393
4	0.016667501519358016
5	0.03426590072995677
6	0.044993388409969116
7	0.06791120299908639
8	0.08852393759084563
9	0.09628141835118514
10	0.11786937909567279
11	0.11924356140179007
12	0.13391628215420365
13	0.12150431293766044
14	0.12376506447353082
15	0.11401280294624687
16	0.11254996371715428
17	0.12478461908774685
18	0.131433888310895
19	0.132586428309574
20	0.12376506447353082
21	0.1299710490818024
22	0.15244557905604314
23	0.13834912830296908
24	0.13524613599883326
25	0.1313452313879197
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2255887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.52531064987344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.47334091816978	38.864165867057835
2	12.335516251672644	12.218405487813003
3	3.7953937416055488	5.639041622748009
4	1.6357301576369925	3.24040176785354
5	0.9111477163490537	2.2562436850054826
6	0.5599847674993637	1.664005174176188
7	0.35834189627746615	1.2422895612404368
8	0.29134558700570606	1.154318456234186
9	0.21425944294013038	0.9550138924150912
>10	1.22638935203913	11.039719181238112
>50	0.09341003015487477	3.217684798209576
>100	0.08623147325443012	9.240754567726817
>500	0.01299410655098716	4.553225150583068
>1k	0.005914558843897595	4.714730787698743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3699	0.16397097904283325	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3096	0.1372409167657777	No Hit
TCCATGTACTCTGCGTTGATACCAC	3013	0.13356165446230242	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2767	0.12265685293633946	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2745	0.12168162678361107	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2497	0.11068816833467278	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2475	0.1097129421819444	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2431	0.1077624898764876	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2269	0.1005812791154876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.4328461487654304E-5	0.0
4	0.0	0.0	0.0	8.865692297530861E-5	0.0
5	0.0	0.0	0.0	8.865692297530861E-5	0.0
6	0.0	0.0	0.0	8.865692297530861E-5	4.4328461487654304E-5
7	0.0	0.0	0.0	1.7731384595061722E-4	4.4328461487654304E-5
8	0.0	0.0	0.0	1.7731384595061722E-4	8.865692297530861E-5
9	0.0	0.0	0.0	1.7731384595061722E-4	1.3298538446296292E-4
10	0.0	0.0	0.0	1.7731384595061722E-4	1.7731384595061722E-4
11	0.0	0.0	0.0	2.216423074382715E-4	1.7731384595061722E-4
12	0.0	0.0	0.0	2.216423074382715E-4	3.9895615338888873E-4
13	0.0	0.0	0.0	2.216423074382715E-4	4.876130763641973E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	190	0.0	13.990535	1
CGTTATT	185	0.0	13.856108	2
TAGAGTG	280	0.0	13.22848	5
GTTATTC	230	0.0	12.797679	3
ACGGTAT	470	0.0	12.735887	9
CAAGACG	505	0.0	12.599632	4
CGCGTAA	160	0.0	12.470557	10
GAGCGTT	115	5.318725E-9	12.392825	7
AACCGCG	215	0.0	12.3736105	7
CCGACCA	385	0.0	12.33945	9
AAGACGG	535	0.0	12.248945	5
CGCATCG	435	0.0	12.231113	13
TTAGAGT	320	0.0	12.167695	4
TACACCG	110	3.8315193E-8	12.087563	5
GTCCTAA	110	3.8529834E-8	12.082736	1
CGTCTTA	190	0.0	12.001739	15
ATCGCCA	480	0.0	11.877776	16
CGACCAT	385	0.0	11.845872	10
TCGCCAG	510	0.0	11.738818	17
GAACCGC	260	0.0	11.693223	6
>>END_MODULE