FastQCFastQC Report
Thu 2 Feb 2017
SRR4062528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255887
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC27550.1221249113984876No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA26360.11684982448145673No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT25600.11348086140839501No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT24100.10683159218524688No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG24050.1066099498778086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG500.002581253221.9998119
ATACGAA9550.021.8846340
CGCAATA9850.021.4414436
CGAATGC9950.020.78374343
TACGAAT10300.020.07749741
CTAGCGG10300.020.07749729
TCTAGCG10350.019.98050728
CAATACG10550.019.60172838
AATACGA10800.019.14798439
TAGATCG1051.7942384E-618.8569815
GCAATAC11550.018.66650837
CCGGTCC12950.018.5172169
ACCGTCG5600.018.4641288
TTAACGG2650.018.26399435
CGGTCCA11650.018.12860110
TAGCGGC11800.018.0845930
GCCGGTC11300.017.7166618
GTATACG750.001289305717.6041411
CGCCGGT11250.017.208747
GCGCAAT12550.016.8285435
Produced by FastQC (version 0.11.5)