##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062528_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2255887
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30554234321134	32.0	32.0	32.0	32.0	32.0
2	31.416527068953364	32.0	32.0	32.0	32.0	32.0
3	31.50234120769347	32.0	32.0	32.0	32.0	32.0
4	31.604884464514402	32.0	32.0	32.0	32.0	32.0
5	31.537837666514324	32.0	32.0	32.0	32.0	32.0
6	35.137682428242194	36.0	36.0	36.0	36.0	36.0
7	35.14827737382236	36.0	36.0	36.0	36.0	36.0
8	35.090961559688054	36.0	36.0	36.0	36.0	36.0
9	35.22500284810365	36.0	36.0	36.0	36.0	36.0
10	35.09291201199351	36.0	36.0	36.0	36.0	36.0
11	35.23426129056996	36.0	36.0	36.0	36.0	36.0
12	35.139636870109186	36.0	36.0	36.0	36.0	36.0
13	35.186735417155205	36.0	36.0	36.0	36.0	36.0
14	35.14475015814179	36.0	36.0	36.0	36.0	36.0
15	35.10028427842352	36.0	36.0	36.0	36.0	36.0
16	35.11708742503503	36.0	36.0	36.0	36.0	36.0
17	35.08663687498532	36.0	36.0	36.0	36.0	36.0
18	35.10027718586968	36.0	36.0	36.0	36.0	36.0
19	35.08735100649988	36.0	36.0	36.0	36.0	36.0
20	35.08706819091559	36.0	36.0	36.0	36.0	36.0
21	35.08200366419063	36.0	36.0	36.0	36.0	36.0
22	35.06258912791288	36.0	36.0	36.0	36.0	36.0
23	35.01885023496301	36.0	36.0	36.0	36.0	36.0
24	34.990222914534286	36.0	36.0	36.0	36.0	36.0
25	34.96516403525531	36.0	36.0	36.0	32.0	36.0
26	34.920314714345174	36.0	36.0	36.0	32.0	36.0
27	34.9040519316792	36.0	36.0	36.0	32.0	36.0
28	34.88675274958364	36.0	36.0	36.0	32.0	36.0
29	34.86092033865172	36.0	36.0	36.0	32.0	36.0
30	34.85646089542605	36.0	36.0	36.0	32.0	36.0
31	34.85650212089524	36.0	36.0	36.0	32.0	36.0
32	34.81654621884873	36.0	36.0	36.0	32.0	36.0
33	34.80157782725819	36.0	36.0	36.0	32.0	36.0
34	34.79504824488106	36.0	36.0	36.0	32.0	36.0
35	34.7633210351405	36.0	36.0	36.0	32.0	36.0
36	34.73953615584468	36.0	36.0	36.0	32.0	36.0
37	34.72618353667537	36.0	36.0	36.0	32.0	36.0
38	34.69131875843072	36.0	36.0	36.0	32.0	36.0
39	34.68040996734322	36.0	36.0	36.0	32.0	36.0
40	34.66515920345301	36.0	36.0	36.0	32.0	36.0
41	34.63981174588975	36.0	36.0	36.0	32.0	36.0
42	34.60575285907495	36.0	36.0	36.0	32.0	36.0
43	34.61222791744444	36.0	36.0	36.0	32.0	36.0
44	34.576472137123886	36.0	36.0	36.0	32.0	36.0
45	34.53241585238977	36.0	36.0	36.0	32.0	36.0
46	34.55100100315308	36.0	36.0	36.0	32.0	36.0
47	34.517519272906846	36.0	36.0	36.0	32.0	36.0
48	34.49044344863018	36.0	36.0	36.0	32.0	36.0
49	34.48057238682611	36.0	36.0	36.0	32.0	36.0
50	33.9774372563874	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	11.0
21	47.0
22	181.0
23	524.0
24	1382.0
25	3298.0
26	6915.0
27	12944.0
28	21771.0
29	33317.0
30	48703.0
31	70551.0
32	104875.0
33	172845.0
34	388278.0
35	1390241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58572209510238	17.331780998409684	11.477140375619534	26.605356530868395
2	16.682078885142474	19.705681301329115	36.162206876006856	27.450032937521556
3	18.49983975204344	23.268446578916688	28.333301564489936	29.898412104549934
4	12.611674526106373	15.088617066642552	35.222923498793826	37.076784908457256
5	14.759655408119027	36.22864807884796	33.14870520939445	15.862991303638571
6	35.60046243635516	34.64531326605428	16.07458382057895	13.679640477011612
7	31.241486157317418	29.901834535005108	20.200586466065424	18.65609284161205
8	28.77391465086682	32.53465266655643	18.862469618380707	19.828963064196035
9	27.368791078631155	13.945733984016043	18.154366774576918	40.53110816277589
10	15.965914959392913	26.154146905407938	31.153821091216006	26.726117043983145
11	38.37098223448249	20.52846618647122	21.506396375350363	19.59415520369593
12	24.638601135606528	23.27035884332859	28.182927602313413	23.90811241875147
13	29.839615193491518	18.661794673226098	25.043408645911786	26.4551814873706
14	23.853278111891242	19.01526982512865	24.364651243612823	32.76680081936728
15	25.728682332049434	26.59619032336283	21.729279879710287	25.945847464877453
16	26.678286634037963	25.263543785659476	22.950528993695162	25.1076405866074
17	24.763297097771297	25.444581222375056	24.596090141039866	25.196031538813777
18	25.74672401587491	24.3008182590706	25.490727150783705	24.461730574270785
19	26.030337512472922	24.71223071013752	24.710235929370576	24.547195848018983
20	25.974040366383598	23.79990664426011	24.58562862412878	25.640424365227517
21	27.0975452227882	23.86848277418151	23.8480473534357	25.185924649594593
22	26.080650316261412	23.908821674135275	24.37604365821515	25.63448435138817
23	24.72437670858514	23.551223975314368	25.023416509780855	26.700982806319644
24	25.092347267394153	24.704428900915694	24.5018921603786	25.70133167131155
25	25.504867929998266	23.868128146489607	24.604202249492108	26.02280167402002
26	25.12781003658428	24.636163070224708	24.964193685233347	25.271833207957666
27	26.0131823978772	24.17288631921723	24.244122156827892	25.56980912607768
28	25.119033001209722	23.991804554040165	25.0767436489505	25.812418795799612
29	24.984717762902132	24.382338299746397	25.0544907612837	25.57845317606777
30	25.16992207499755	24.42418436739074	25.26952812796031	25.1363654296514
31	25.589402306055227	24.23428123837763	24.21167372301893	25.964642732548214
32	25.298518941773235	24.28836196139257	24.20994491302091	26.20317418381328
33	24.9000947299222	23.864271570340183	24.86791226688216	26.367721432855458
34	25.518698409982417	24.143141921559014	25.07022736511182	25.26793230334675
35	26.29231871986496	24.00000531941538	24.734040313189446	24.97363564753022
36	25.087471136630512	24.583811157207787	24.504507539606372	25.82421016655533
37	26.329953583667976	24.20409355610454	24.335438787492457	25.13051407273503
38	25.24346299260557	24.034847490144674	24.810418252332674	25.91127126491708
39	25.696366883624933	23.99752292557207	24.318815614434588	25.98729457636841
40	25.94872881487415	24.20054727918553	24.76604546238353	25.08467844355679
41	24.90293175145741	24.526982069580612	25.15901727347159	25.411068905490392
42	26.40659749358013	24.591568637968127	24.483939133476103	24.51789473497564
43	25.51413257844919	23.50849133844027	24.79875986696142	26.178616216149127
44	25.120362855054353	24.166370035378545	24.81489542694293	25.898371682624173
45	25.18809674420749	24.49355840961892	24.662582833271347	25.655762012902244
46	24.93034884313695	24.039789262307252	25.069052722102413	25.96080917245338
47	25.26221393181485	23.94632355255383	25.338059929420226	25.453402586211098
48	26.086058388562904	24.805852420799447	24.092474490078626	25.015614700559023
49	24.949166336789034	24.638867106375454	24.69693739092428	25.715029165911236
50	24.893290216478235	25.004599081956748	24.373981829747528	25.72812887181749
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	23.5
2	29.0
3	138.5
4	248.0
5	269.5
6	291.0
7	262.0
8	233.0
9	315.5
10	398.0
11	589.5
12	781.0
13	1478.5
14	2176.0
15	2857.0
16	3538.0
17	3964.5
18	4391.0
19	4572.5
20	4754.0
21	5654.5
22	6555.0
23	7242.0
24	7929.0
25	9670.5
26	11412.0
27	14633.5
28	17855.0
29	20377.5
30	22900.0
31	25393.0
32	27886.0
33	32474.5
34	37063.0
35	43910.0
36	50757.0
37	62424.0
38	74091.0
39	82340.0
40	90589.0
41	100473.0
42	110357.0
43	114822.0
44	119287.0
45	133645.5
46	148004.0
47	159468.0
48	170932.0
49	175132.0
50	179332.0
51	172328.5
52	165325.0
53	162339.5
54	159354.0
55	162103.5
56	164853.0
57	161890.5
58	158928.0
59	146157.0
60	133386.0
61	117859.5
62	102333.0
63	90331.5
64	78330.0
65	67245.0
66	56160.0
67	50355.5
68	44551.0
69	41320.5
70	38090.0
71	29694.0
72	21298.0
73	18976.0
74	16654.0
75	12256.0
76	7858.0
77	7113.5
78	6369.0
79	5390.5
80	4412.0
81	3451.5
82	2491.0
83	2102.0
84	1713.0
85	1461.0
86	1209.0
87	879.0
88	549.0
89	333.5
90	118.0
91	86.0
92	54.0
93	40.0
94	26.0
95	16.5
96	7.0
97	8.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01595824613555555
2	0.004477174610253085
3	3.5462769190123443E-4
4	4.4328461487654304E-5
5	4.4328461487654304E-5
6	3.9895615338888873E-4
7	8.865692297530861E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.865692297530861E-5
47	0.0
48	0.0
49	0.0
50	8.865692297530861E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2255887.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.666623128975274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.43418808990177	40.52429636468115
2	12.692048664531011	13.115105901698751
3	3.6811307851695556	5.705747908974727
4	1.5977702291902776	3.3020556911308208
5	0.8793852029856578	2.27174319339287
6	0.5333403658345486	1.6533537408626051
7	0.3697053401029849	1.3370998533109386
8	0.26935630266013216	1.1133384461564197
9	0.20326697290738166	0.9451906275395981
>10	1.16136427635809	10.786873932326948
>50	0.08654553072188022	3.1088876653954887
>100	0.0764142160136716	8.553838309261787
>500	0.01049472712223206	3.751500491052616
>1k	0.004989296500733252	3.8309678742153603
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2755	0.1221249113984876	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2636	0.11684982448145673	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2560	0.11348086140839501	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2410	0.10683159218524688	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2405	0.1066099498778086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.4328461487654304E-5
2	0.0	0.0	0.0	0.0	4.4328461487654304E-5
3	0.0	0.0	0.0	0.0	4.4328461487654304E-5
4	0.0	0.0	0.0	4.4328461487654304E-5	4.4328461487654304E-5
5	0.0	0.0	0.0	4.4328461487654304E-5	4.4328461487654304E-5
6	0.0	0.0	0.0	4.4328461487654304E-5	1.3298538446296292E-4
7	0.0	0.0	0.0	4.4328461487654304E-5	1.3298538446296292E-4
8	0.0	0.0	0.0	1.3298538446296292E-4	1.3298538446296292E-4
9	0.0	0.0	0.0	1.3298538446296292E-4	1.3298538446296292E-4
10	0.0	0.0	0.0	1.3298538446296292E-4	1.3298538446296292E-4
11	0.0	0.0	0.0	1.7731384595061722E-4	1.3298538446296292E-4
12	0.0	0.0	0.0	1.7731384595061722E-4	2.6597076892592584E-4
13	0.0	0.0	0.0	1.7731384595061722E-4	3.9895615338888873E-4
14	0.0	0.0	0.0	1.7731384595061722E-4	3.9895615338888873E-4
15	0.0	0.0	0.0	1.7731384595061722E-4	6.205984608271603E-4
16	0.0	0.0	0.0	1.7731384595061722E-4	6.205984608271603E-4
17	0.0	0.0	0.0	1.7731384595061722E-4	6.205984608271603E-4
18	0.0	0.0	0.0	2.216423074382715E-4	7.535838452901231E-4
19	0.0	0.0	0.0	2.6597076892592584E-4	7.535838452901231E-4
20	0.0	0.0	0.0	4.43284614876543E-4	8.86569229753086E-4
21	0.0	0.0	0.0	6.205984608271603E-4	9.308976912407403E-4
22	0.0	0.0	0.0	7.535838452901231E-4	9.308976912407403E-4
23	0.0	0.0	0.0	0.0010638830757037033	0.0010638830757037033
24	0.0	0.0	0.0	0.0018617953824814806	0.0010638830757037033
25	0.0	0.0	0.0	0.002349408458845678	0.0010638830757037033
26	0.0	0.0	0.0	0.003413291534549381	0.0011525399986790118
27	0.0	0.0	0.0	0.00474314537917901	0.001196868460166666
28	0.0	0.0	0.0	0.008466736144141972	0.001196868460166666
29	0.0	0.0	0.0	0.015381976136216042	0.001196868460166666
30	0.0	0.0	0.0	0.028990813812925915	0.001196868460166666
31	0.0	0.0	0.0	0.06303507223544441	0.001196868460166666
32	0.0	0.0	0.0	0.09885246911746909	0.0012411969216543205
33	0.0	0.0	0.0	0.13546777830627155	0.0012411969216543205
34	0.0	0.0	0.0	0.17908698441012338	0.001329853844629629
35	0.0	0.0	0.0	0.22882351819927152	0.001329853844629629
36	0.0	0.0	0.0	0.30249742119175294	0.001329853844629629
37	0.0	0.0	0.0	0.41540201260080845	0.001329853844629629
38	0.0	0.0	0.0	0.5629714608932096	0.0015071676905802462
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACG	50	0.0025812532	21.999811	9
ATACGAA	955	0.0	21.88463	40
CGCAATA	985	0.0	21.44144	36
CGAATGC	995	0.0	20.783743	43
TACGAAT	1030	0.0	20.077497	41
CTAGCGG	1030	0.0	20.077497	29
TCTAGCG	1035	0.0	19.980507	28
CAATACG	1055	0.0	19.601728	38
AATACGA	1080	0.0	19.147984	39
TAGATCG	105	1.7942384E-6	18.856981	5
GCAATAC	1155	0.0	18.666508	37
CCGGTCC	1295	0.0	18.517216	9
ACCGTCG	560	0.0	18.464128	8
TTAACGG	265	0.0	18.263994	35
CGGTCCA	1165	0.0	18.128601	10
TAGCGGC	1180	0.0	18.08459	30
GCCGGTC	1130	0.0	17.716661	8
GTATACG	75	0.0012893057	17.604141	1
CGCCGGT	1125	0.0	17.20874	7
GCGCAAT	1255	0.0	16.82854	35
>>END_MODULE