FastQCFastQC Report
Thu 2 Feb 2017
SRR4062527_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062527_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973238
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33740.17098799029817993No Hit
TATCAACGCAGAGTACTTTTTTTTT21490.10890728842643412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA2150.011.93133215
ACAATAC1950.011.6874543
CCTTATA907.506338E-611.6051132
CCGACCA2600.011.3279679
AAGACGG4450.011.3133545
GAGCGAA3950.011.065433516
GTATCAA26950.010.9916281
GCGCAAG4250.010.946451
CAAGACG4600.010.9436064
CCGTCGT2350.010.9158999
CGAGCCG3050.010.90264415
GTCTAAC803.792357E-410.6811671
AATAGCG2850.010.6654845
ACCGTCG2500.010.641258
CGTCGTA2500.010.6407110
TAGGGTC2950.010.6251324
GATATAC2600.010.5898751
AGGCCCG3500.010.58642110
TGTAGGA6400.010.5336182
GCGAAAG4150.010.53296318
Produced by FastQC (version 0.11.5)