##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062527_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1973238
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16514277547868	32.0	32.0	32.0	32.0	32.0
2	30.7779467048577	32.0	32.0	32.0	32.0	32.0
3	30.75360144088042	32.0	32.0	32.0	32.0	32.0
4	30.836685691234408	32.0	32.0	32.0	32.0	32.0
5	30.61539915610788	32.0	32.0	32.0	32.0	32.0
6	34.27376677319209	36.0	36.0	36.0	32.0	36.0
7	34.14672279775678	36.0	36.0	36.0	32.0	36.0
8	34.16082043828469	36.0	36.0	36.0	32.0	36.0
9	34.32375618146417	36.0	36.0	36.0	32.0	36.0
10	33.96489222283374	36.0	36.0	36.0	32.0	36.0
11	34.3142018347508	36.0	36.0	36.0	32.0	36.0
12	34.0689931979822	36.0	36.0	36.0	32.0	36.0
13	34.193653781246866	36.0	36.0	36.0	32.0	36.0
14	34.09629755761849	36.0	36.0	36.0	32.0	36.0
15	34.022932357880805	36.0	36.0	36.0	32.0	36.0
16	34.03712172581311	36.0	36.0	36.0	32.0	36.0
17	33.95852502333727	36.0	36.0	36.0	32.0	36.0
18	33.97146770941975	36.0	36.0	36.0	32.0	36.0
19	33.96472295789965	36.0	36.0	36.0	32.0	36.0
20	33.969933682606964	36.0	36.0	36.0	32.0	36.0
21	33.94733326643821	36.0	36.0	36.0	32.0	36.0
22	33.89443087959992	36.0	36.0	36.0	32.0	36.0
23	33.855920573189856	36.0	36.0	36.0	32.0	36.0
24	33.82647506281553	36.0	36.0	36.0	32.0	36.0
25	33.382565610433204	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	85.0
5	208.0
6	337.0
7	78.0
8	268.0
9	371.0
10	253.0
11	85.0
12	127.0
13	102.0
14	281.0
15	373.0
16	541.0
17	751.0
18	1056.0
19	1564.0
20	2402.0
21	3708.0
22	5780.0
23	8859.0
24	12820.0
25	17633.0
26	25082.0
27	32602.0
28	43546.0
29	57697.0
30	73688.0
31	100304.0
32	142402.0
33	203429.0
34	443847.0
35	792951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05018314071501	17.646939570912988	10.919362841618126	26.38351444675387
2	16.86500183714065	20.206266550103262	36.86981641263446	26.05891520012163
3	19.140320522371795	23.83853465061521	28.50654333113381	28.514601495879187
4	12.814280993210051	15.445665198112843	36.0894229658029	35.6506308428742
5	14.6113378652619	36.41265526434926	33.837831041767316	15.138175828621517
6	33.78380844988156	35.53284307362819	17.074484051010806	13.608864425479444
7	29.689942136731766	30.639488003894737	21.156149683805893	18.514420175567604
8	27.81739328920338	32.98435161936646	19.95003677496259	19.248218316467575
9	27.427040901735396	14.168649273833136	18.914934738828496	39.48937508560297
10	15.858007800509109	26.86198213300847	31.974679549464025	25.305330517018394
11	37.07048156397936	21.316575928397103	22.577237463658182	19.035705043965358
12	24.348350311121305	23.90605161639976	29.135284738665206	22.610313333813725
13	29.41890413572367	19.703596660895826	25.364263142192012	25.513236061188493
14	23.067094921325964	20.01507009879287	25.34521689271815	31.57261808716302
15	24.944367607081574	27.436333508204573	22.39141662954458	25.22788225516927
16	25.363324344823912	25.944186683645903	24.200584971854887	24.491903999675298
17	23.79604621225998	25.98691204012139	25.66131136344839	24.555730384170236
18	24.498964056332195	25.14392335183976	26.643770865397347	23.713341726430695
19	25.30188223497389	25.23966881757971	25.495880517761776	23.96256842968463
20	25.224425262277684	24.83971073851793	25.410541441801655	24.525322557402735
21	26.164635198359942	24.594399845736962	24.607948606050766	24.633016349852333
22	25.39622277602107	25.03550346913812	25.046009856717237	24.52226389812357
23	24.27190230826266	24.722511638245816	25.677626807778758	25.327959245712766
24	24.431622402711913	25.44995978310789	25.434076013732103	24.684341800448095
25	24.577502811235195	25.01512180666009	25.547074488598764	24.860300893505947
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	201.0
1	201.0
2	607.5
3	1014.0
4	1014.0
5	1014.0
6	2553.0
7	4092.0
8	4092.0
9	4092.0
10	4495.5
11	4899.0
12	4899.0
13	4899.0
14	5932.5
15	6966.0
16	6966.0
17	6966.0
18	11329.5
19	15693.0
20	15693.0
21	15693.0
22	24526.5
23	33360.0
24	33360.0
25	33360.0
26	48911.5
27	64463.0
28	64463.0
29	64463.0
30	79727.0
31	94991.0
32	94991.0
33	94991.0
34	113605.0
35	132219.0
36	132219.0
37	132219.0
38	148603.0
39	164987.0
40	164987.0
41	164987.0
42	184432.0
43	203877.0
44	203877.0
45	203877.0
46	228508.5
47	253140.0
48	253140.0
49	253140.0
50	253350.5
51	253561.0
52	253561.0
53	253561.0
54	235976.0
55	218391.0
56	218391.0
57	218391.0
58	199275.5
59	180160.0
60	180160.0
61	180160.0
62	159350.5
63	138541.0
64	138541.0
65	138541.0
66	116032.5
67	93524.0
68	93524.0
69	93524.0
70	71582.0
71	49640.0
72	49640.0
73	49640.0
74	39050.5
75	28461.0
76	28461.0
77	28461.0
78	23013.5
79	17566.0
80	17566.0
81	17566.0
82	12501.0
83	7436.0
84	7436.0
85	7436.0
86	5545.5
87	3655.0
88	3655.0
89	3655.0
90	2488.0
91	1321.0
92	1321.0
93	1321.0
94	793.5
95	266.0
96	266.0
97	266.0
98	540.0
99	814.0
100	814.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008361890456194336
2	0.0031927218105469285
3	0.004256962414062571
4	0.01631835592057319
5	0.03623485864350879
6	0.048144217778088604
7	0.06831411112090888
8	0.09061248567076045
9	0.09770742302753138
10	0.11863748823000571
11	0.12000579757738296
12	0.13297939731547842
13	0.12213427878441424
14	0.12406004749553778
15	0.11473527268378168
16	0.11164390712118863
17	0.12259038190020667
18	0.13029345674470083
19	0.13186447858798583
20	0.12294512876804521
21	0.1306482036125394
22	0.1524397969226216
23	0.14164535651553437
24	0.13546262539034826
25	0.13044549111663165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1973238.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.9350437218751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.79071230936982	45.190320253460335
2	12.115497990916973	13.553618196684617
3	3.199032551436414	5.368140768968924
4	1.2754229300283055	2.8536334942006127
5	0.6528102788454415	1.8257485744654622
6	0.38871753548156823	1.3045759405572632
7	0.26403512541915847	1.0338171399101985
8	0.19338471472695964	0.8653585978715865
9	0.1443333146379531	0.7265961238317356
>10	0.7924946138665994	8.263410358385622
>50	0.08632209003762478	3.388373500143967
>100	0.0851401424678138	9.993427289356681
>500	0.009276940466649888	3.3896775965258814
>1k	0.0028194622986877113	2.2433021656371794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3374	0.17098799029817993	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2149	0.10890728842643412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.067812397693537E-5	0.0
5	0.0	0.0	0.0	1.0135624795387074E-4	0.0
6	0.0	0.0	0.0	1.0135624795387074E-4	0.0
7	0.0	0.0	0.0	1.5203437193080611E-4	0.0
8	0.0	0.0	0.0	2.533906198846769E-4	0.0
9	0.0	0.0	0.0	4.05424991815483E-4	0.0
10	0.0	0.0	0.0	4.05424991815483E-4	0.0
11	0.0	0.0	0.0	4.05424991815483E-4	0.0
12	0.0	0.0	0.0	4.05424991815483E-4	0.0
13	0.0	0.0	0.0	4.5610311579241837E-4	1.0135624795387074E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	215	0.0	11.931332	15
ACAATAC	195	0.0	11.687454	3
CCTTATA	90	7.506338E-6	11.605113	2
CCGACCA	260	0.0	11.327967	9
AAGACGG	445	0.0	11.313354	5
GAGCGAA	395	0.0	11.0654335	16
GTATCAA	2695	0.0	10.991628	1
GCGCAAG	425	0.0	10.94645	1
CAAGACG	460	0.0	10.943606	4
CCGTCGT	235	0.0	10.915899	9
CGAGCCG	305	0.0	10.902644	15
GTCTAAC	80	3.792357E-4	10.681167	1
AATAGCG	285	0.0	10.665484	5
ACCGTCG	250	0.0	10.64125	8
CGTCGTA	250	0.0	10.64071	10
TAGGGTC	295	0.0	10.625132	4
GATATAC	260	0.0	10.589875	1
AGGCCCG	350	0.0	10.586421	10
TGTAGGA	640	0.0	10.533618	2
GCGAAAG	415	0.0	10.532963	18
>>END_MODULE