FastQCFastQC Report
Thu 2 Feb 2017
SRR4062527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973238
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA5300.017.84898636
TAGCGGC5450.017.76139630
GTATCAA33500.017.6017131
CTATACA2150.017.395724
TAGGACG5700.017.3687924
TCTAGCG5500.017.1999328
CTAGCGG5500.017.1999329
CAATACG5700.016.98238838
ATACGAA5700.016.59642440
GAGCGAA7300.016.57527516
TTAGGAC5850.016.5473613
ACTATAC2101.5097612E-1015.7146213
AATACGA6300.015.36501739
TATGGCG7750.015.3289716
CGAATGC6050.015.27266543
GCGAAAG8100.014.93821218
TATACTG3550.014.873185
AGCGAAA8000.014.8499417
GCAATAC6400.014.7811937
ATTAGAC1057.1605074E-414.6669793
Produced by FastQC (version 0.11.5)