FastQCFastQC Report
Thu 2 Feb 2017
SRR4062526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197227
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG48910.40852737200213496No Hit
GTATCAACGCAGAGTACTTTTTTTT47310.395163156193437No Hit
TCCATGTACTCTGCGTTGATACCAC40170.3355253431471225No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35670.29793848618515956No Hit
TATCAACGCAGAGTACTTTTTTTTT30390.2538365740164564No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25250.21090403073101427No Hit
GAGTACATGGAAGCAGTGGTATCAA21580.18024986071981336No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18740.15652837765937455No Hit
CATGTACTCTGCGTTGATACCACTG17940.14984626975502557No Hit
GGTATCAACGCAGAGTACTTTTTTT15480.1292987879491525No Hit
GTACATGGGGTGGTATCAACGCAAA15030.12554010225295623No Hit
GAATAGGACCGCGGTTCTATTTTGT12830.10716430551599655No Hit
GCGCAAGACGGACCAGAGCGAAAGC12740.10641256837675729No Hit
GTCTTGCGCCGGTCCAAGAATTTCA12630.10549377853990931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG350.002172230716.2829935
GCGTTAT1050.016.2789121
ATTCGCA400.005275236414.25119114
ACTCTAA2200.013.81933710
CTTACAC707.2843104E-613.5668933
TACGCTA1700.013.4128869
GATCGAA500.00149872313.30055711
CGAGCCG1950.013.15494615
TAACCTA802.0020598E-613.0603165
ATACGCT1750.013.0296618
TCCAACG905.38781E-712.66931318
ACGCTAT1800.012.66772610
GTTATTC1501.8189894E-1212.6624333
AACACTC2650.012.5482197
CGTTATT1301.9826984E-1012.4178882
CAAGACG2700.012.3122424
GCTCGTA853.937488E-612.2951459
AAGACGG2800.012.2122455
GCGTAAC550.003066775612.09141411
ATATACG1900.012.06
Produced by FastQC (version 0.11.5)