FastQCFastQC Report
Thu 2 Feb 2017
SRR4062526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197227
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20470.17097843600252918No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC19170.1601200106579621No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13000.1085842534456707No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12600.10524319949349623No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12140.10140098744849556No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12130.10131746109969121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG4850.021.3195064
TTAGGAC3200.021.312423
TACCGTC2300.019.1303627
AGGACGT5550.019.0269555
ACGGAAT4100.018.78041618
GGACGTG5200.018.6153146
GATATAC3800.018.529341
ATACCGT2500.018.479936
AATCGAG4200.018.33326322
GTCCTAA2900.018.209871
GACGTGA2700.017.9258587
ATACACT3800.017.3683574
CGTCGTA2550.017.25483710
ACCGTCG2550.017.2548378
TTAGAGA2055.456968E-1217.1706684
GTTATAT2201.8189894E-1217.0027771
GATATAT2500.016.722731
CTAGACA2251.8189894E-1216.6221584
TCTACAC4500.016.6221583
TTAACGG2650.016.6037135
Produced by FastQC (version 0.11.5)