##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062526_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197227
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.181847719772442	32.0	32.0	32.0	32.0	32.0
2	31.411094136700893	32.0	32.0	32.0	32.0	32.0
3	31.50950237507173	32.0	32.0	32.0	32.0	32.0
4	31.61399216689901	32.0	32.0	32.0	32.0	32.0
5	31.548710478463985	32.0	32.0	32.0	32.0	32.0
6	35.14337631877664	36.0	36.0	36.0	36.0	36.0
7	35.15380708921533	36.0	36.0	36.0	36.0	36.0
8	35.09840907363432	36.0	36.0	36.0	36.0	36.0
9	35.20983405820283	36.0	36.0	36.0	36.0	36.0
10	35.10424004804435	36.0	36.0	36.0	36.0	36.0
11	35.221061670009114	36.0	36.0	36.0	36.0	36.0
12	35.13392364188245	36.0	36.0	36.0	36.0	36.0
13	35.17282353304762	36.0	36.0	36.0	36.0	36.0
14	35.13050908474333	36.0	36.0	36.0	36.0	36.0
15	35.098075803502596	36.0	36.0	36.0	36.0	36.0
16	35.109325967423054	36.0	36.0	36.0	36.0	36.0
17	35.08509831468886	36.0	36.0	36.0	36.0	36.0
18	35.10167745966304	36.0	36.0	36.0	36.0	36.0
19	35.0726253250219	36.0	36.0	36.0	36.0	36.0
20	35.05393296342297	36.0	36.0	36.0	36.0	36.0
21	35.0339793539571	36.0	36.0	36.0	36.0	36.0
22	35.020404651749416	36.0	36.0	36.0	36.0	36.0
23	34.98023933639986	36.0	36.0	36.0	36.0	36.0
24	34.964482925961406	36.0	36.0	36.0	36.0	36.0
25	34.937919041251156	36.0	36.0	36.0	32.0	36.0
26	34.89181667302859	36.0	36.0	36.0	32.0	36.0
27	34.87282862815489	36.0	36.0	36.0	32.0	36.0
28	34.85313060931636	36.0	36.0	36.0	32.0	36.0
29	34.81863172146969	36.0	36.0	36.0	32.0	36.0
30	34.804560037486624	36.0	36.0	36.0	32.0	36.0
31	34.79050672930029	36.0	36.0	36.0	32.0	36.0
32	34.75013677439617	36.0	36.0	36.0	32.0	36.0
33	34.71490034888956	36.0	36.0	36.0	32.0	36.0
34	34.67765260890374	36.0	36.0	36.0	32.0	36.0
35	34.63270541008514	36.0	36.0	36.0	32.0	36.0
36	34.595613029108094	36.0	36.0	36.0	32.0	36.0
37	34.5724820773337	36.0	36.0	36.0	32.0	36.0
38	34.524590574719745	36.0	36.0	36.0	32.0	36.0
39	34.47102512723151	36.0	36.0	36.0	32.0	36.0
40	34.4323866735381	36.0	36.0	36.0	32.0	36.0
41	34.40704979089178	36.0	36.0	36.0	32.0	36.0
42	34.33405527940817	36.0	36.0	36.0	32.0	36.0
43	34.33990964119587	36.0	36.0	36.0	32.0	36.0
44	34.29887314602828	36.0	36.0	36.0	32.0	36.0
45	34.202723460129114	36.0	36.0	36.0	32.0	36.0
46	34.20409663330346	36.0	36.0	36.0	32.0	36.0
47	34.135908227930045	36.0	36.0	36.0	32.0	36.0
48	34.09449502892935	36.0	36.0	36.0	32.0	36.0
49	34.081980276088	36.0	36.0	36.0	32.0	36.0
50	33.54297973567252	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	0.0
20	15.0
21	33.0
22	110.0
23	315.0
24	916.0
25	2196.0
26	4775.0
27	8837.0
28	14562.0
29	21536.0
30	29970.0
31	41294.0
32	60402.0
33	93753.0
34	201760.0
35	716751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.51448788047133	18.027626594042783	11.561232545414295	25.896652980071593
2	15.942561638652197	20.925609286497675	36.713975103138296	26.41785397171183
3	18.827580964854324	24.98728300717414	28.500931744203434	27.684204283768103
4	12.261876024767314	15.817270466738446	35.880366481432446	36.04048702706179
5	13.96543846739173	37.187851593724496	33.43827026954788	15.408439669335891
6	33.895831836808334	35.37317347383684	17.01035233349315	13.720642355861672
7	29.63673555641495	30.767598792877205	21.166579103211003	18.42908654749684
8	28.48532483814682	32.39185217172683	19.831243364875668	19.291579625250684
9	27.669606515723416	14.075025037023053	18.812556014857666	39.44281243239586
10	16.100204890133618	26.553443916650725	31.695409475396062	25.6509417178196
11	37.22502081894244	21.181029161554157	22.260857798896954	19.333092220606453
12	24.51899263882288	24.0407207655691	28.893351052056126	22.54693554355189
13	29.72869806644855	19.513926765767895	25.682932309411665	25.07444285837189
14	23.586003322678156	19.85028737240306	25.32310079876247	31.24060850615631
15	25.324270167645736	26.969906291789275	23.326904588687025	24.378918951877964
16	25.64434313626405	25.58637585019382	24.467790987005806	24.30149002653632
17	23.465725380399874	25.693707208407428	26.050364717802054	24.790202693390643
18	24.67025885650758	24.206771146992175	27.68305425788092	23.439915738619327
19	25.259954879066377	25.341476595499437	25.87253712119757	23.526031404236626
20	25.076280438045583	24.125332956907922	26.572905555922144	24.22548104912435
21	26.414205493193855	24.200256091785434	24.85986366829348	24.52567474672723
22	25.04637800517362	24.769571685235967	25.60650570025567	24.577544609334737
23	24.022428495180947	24.33348061812839	26.151849231599357	25.49224165509131
24	24.231160840843046	25.655201561608614	25.758440128730808	24.355197468817526
25	24.800392907944776	24.542630595534515	25.908035819439423	24.748940677081286
26	23.648230452537405	25.35459023226172	26.773619372099027	24.22355994310185
27	24.985904928639265	25.216938809432126	25.79427293236788	24.002883329560724
28	23.902067026553862	24.652217165165837	26.48495231063115	24.960763497649154
29	23.730169800714485	25.099584289362	26.608738359559215	24.5615075503643
30	24.35511394246872	24.999352670796764	26.712979242867057	23.932554143867453
31	24.091838891037373	25.079955597392978	25.733883382182327	25.094322129387326
32	23.878345543493424	25.2814211507091	25.819999047799623	25.02023425799786
33	23.687905468219476	24.791706167669123	26.27012254150633	25.250265822605073
34	24.19749972227489	25.201904066647344	26.768691317519565	23.831904893558196
35	25.166655947451904	24.906053739182294	26.147171756066314	23.78011855729949
36	23.8893710215356	25.53191667077338	26.001167698356287	24.577544609334737
37	25.11453550579798	25.251936349581154	25.52523456286903	24.108293581751834
38	23.95719441676474	25.3733836607427	25.822504838263754	24.846917084228807
39	24.331058354013066	25.1110273991482	25.641419714055896	24.916494532782842
40	25.0881411795758	25.17467447693712	25.89475512997953	23.842429213507547
41	23.70887058176937	25.361773498258895	26.657768326307373	24.27158759366436
42	25.204743962506694	25.906365292463335	25.676417254204924	23.212473490825047
43	24.075384200322915	24.768068210957487	25.881808545914854	25.27473904280475
44	23.757984074866336	25.27265088408464	26.165464026454465	24.803901014594558
45	23.951681677743654	25.513290294990004	25.92348819396823	24.611539833298114
46	23.82415684256774	25.02869132477911	26.104177490298408	25.042974342354746
47	24.441730766178846	24.82210975863391	26.28540786333753	24.450751611849718
48	24.548978598043647	26.31723140223199	24.917580375317296	24.216209624407067
49	23.558021995828692	25.635823448686008	26.062308985681078	24.743845569804222
50	23.241373607511356	26.663030486282054	25.053644797519603	25.041951108686987
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	55.0
2	75.0
3	197.5
4	320.0
5	374.5
6	429.0
7	390.5
8	352.0
9	476.0
10	600.0
11	832.5
12	1065.0
13	1917.0
14	2769.0
15	3835.5
16	4902.0
17	5786.0
18	6670.0
19	7303.5
20	7937.0
21	8891.0
22	9845.0
23	9810.0
24	9775.0
25	10568.5
26	11362.0
27	13853.0
28	16344.0
29	18185.5
30	20027.0
31	20923.0
32	21819.0
33	24854.0
34	27889.0
35	32058.0
36	36227.0
37	42000.5
38	47774.0
39	49589.5
40	51405.0
41	55534.5
42	59664.0
43	59820.5
44	59977.0
45	67299.5
46	74622.0
47	80641.5
48	86661.0
49	86548.5
50	86436.0
51	81683.0
52	76930.0
53	75595.0
54	74260.0
55	75928.0
56	77596.0
57	76474.5
58	75353.0
59	69640.0
60	63927.0
61	57235.0
62	50543.0
63	44188.5
64	37834.0
65	32815.5
66	27797.0
67	24370.0
68	20943.0
69	19029.0
70	17115.0
71	13357.0
72	9599.0
73	8783.5
74	7968.0
75	5862.0
76	3756.0
77	3342.5
78	2929.0
79	2595.0
80	2261.0
81	1803.5
82	1346.0
83	1168.0
84	990.0
85	821.0
86	652.0
87	477.0
88	302.0
89	199.0
90	96.0
91	60.0
92	24.0
93	15.5
94	7.0
95	6.5
96	6.0
97	6.0
98	6.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01603705897043752
2	0.00400926474260938
3	8.352634880436209E-4
4	3.341053952174483E-4
5	0.0
6	7.517371392392587E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.352634880436208E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1197227.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.21537693316235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76418593754163	38.80291340825393
2	14.380833429725826	14.7303960943286
3	4.553729409222735	6.996629044349074
4	1.9087222496945389	3.910237179152778
5	0.9799496745196636	2.5094245978027168
6	0.5503341661188107	1.6911343054183487
7	0.3575703965764968	1.281917184856394
8	0.22664155140139589	0.9286025986991344
9	0.18267953984949065	0.8420401342231484
>10	0.8702326807168868	8.250485264927738
>50	0.1037234874597365	3.7932225149819345
>100	0.11089823837012994	11.961049852357716
>500	0.008530631527279573	3.0017516852527355
>1k	0.0019686072755260554	1.3001961353958096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2047	0.17097843600252918	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1917	0.1601200106579621	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1300	0.1085842534456707	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1260	0.10524319949349623	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1214	0.10140098744849556	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1213	0.10131746109969121	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.352634880436208E-5
2	0.0	0.0	0.0	0.0	8.352634880436208E-5
3	0.0	0.0	0.0	0.0	8.352634880436208E-5
4	0.0	0.0	0.0	0.0	8.352634880436208E-5
5	0.0	0.0	0.0	0.0	8.352634880436208E-5
6	0.0	0.0	0.0	0.0	1.6705269760872415E-4
7	0.0	0.0	0.0	0.0	1.6705269760872415E-4
8	0.0	0.0	0.0	0.0	1.6705269760872415E-4
9	0.0	0.0	0.0	0.0	2.505790464130862E-4
10	8.352634880436208E-5	0.0	0.0	0.0	2.505790464130862E-4
11	8.352634880436208E-5	0.0	0.0	0.0	3.341053952174483E-4
12	8.352634880436208E-5	0.0	0.0	0.0	4.176317440218104E-4
13	8.352634880436208E-5	0.0	0.0	0.0	4.176317440218104E-4
14	8.352634880436208E-5	0.0	0.0	8.352634880436208E-5	5.846844416305346E-4
15	8.352634880436208E-5	0.0	0.0	8.352634880436208E-5	6.682107904348966E-4
16	8.352634880436208E-5	0.0	0.0	2.505790464130862E-4	7.517371392392587E-4
17	8.352634880436208E-5	0.0	0.0	2.505790464130862E-4	7.517371392392587E-4
18	8.352634880436208E-5	0.0	0.0	3.341053952174483E-4	7.517371392392587E-4
19	8.352634880436208E-5	0.0	0.0	3.341053952174483E-4	7.517371392392587E-4
20	8.352634880436208E-5	0.0	0.0	6.682107904348966E-4	8.352634880436208E-4
21	8.352634880436208E-5	0.0	0.0	0.0012528952320654313	9.187898368479829E-4
22	8.352634880436208E-5	0.0	0.0	0.0025057904641308626	9.187898368479829E-4
23	8.352634880436208E-5	0.0	0.0	0.00442689648663119	0.001002316185652345
24	8.352634880436208E-5	0.0	0.0	0.00843616122924057	0.001002316185652345
25	8.352634880436208E-5	0.0	0.0	0.010607846298153985	0.001002316185652345
26	8.352634880436208E-5	0.0	0.0	0.01445005834315464	0.0012528952320654313
27	8.352634880436208E-5	0.0	0.0	0.020380429108264347	0.0012528952320654313
28	8.352634880436208E-5	0.0	0.0	0.030821222708809607	0.0012528952320654313
29	8.352634880436208E-5	0.0	0.0	0.05120165181707396	0.0012528952320654313
30	8.352634880436208E-5	0.0	0.0	0.0819393481770792	0.0013364215808697932
31	8.352634880436208E-5	0.0	0.0	0.1490945326157863	0.0013364215808697932
32	8.352634880436208E-5	0.0	0.0	0.2288621957239521	0.0014199479296741553
33	8.352634880436208E-5	0.0	0.0	0.3026994880670082	0.0014199479296741553
34	8.352634880436208E-5	0.0	0.0	0.3835529937096307	0.0014199479296741553
35	8.352634880436208E-5	0.0	0.0	0.47810482055616854	0.0014199479296741553
36	8.352634880436208E-5	0.0	0.0	0.6039790282043422	0.0014199479296741553
37	8.352634880436208E-5	0.0	0.0	0.7980107364768753	0.0014199479296741553
38	8.352634880436208E-5	0.0	0.0	1.037397252150177	0.0015034742784785175
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	485	0.0	21.319506	4
TTAGGAC	320	0.0	21.31242	3
TACCGTC	230	0.0	19.130362	7
AGGACGT	555	0.0	19.026955	5
ACGGAAT	410	0.0	18.780416	18
GGACGTG	520	0.0	18.615314	6
GATATAC	380	0.0	18.52934	1
ATACCGT	250	0.0	18.47993	6
AATCGAG	420	0.0	18.333263	22
GTCCTAA	290	0.0	18.20987	1
GACGTGA	270	0.0	17.925858	7
ATACACT	380	0.0	17.368357	4
CGTCGTA	255	0.0	17.254837	10
ACCGTCG	255	0.0	17.254837	8
TTAGAGA	205	5.456968E-12	17.170668	4
GTTATAT	220	1.8189894E-12	17.002777	1
GATATAT	250	0.0	16.72273	1
CTAGACA	225	1.8189894E-12	16.622158	4
TCTACAC	450	0.0	16.622158	3
TTAACGG	265	0.0	16.60371	35
>>END_MODULE