FastQCFastQC Report
Thu 2 Feb 2017
SRR4062525_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062525_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083464
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG48260.4454231981865572No Hit
TCCATGTACTCTGCGTTGATACCAC41430.3823846477594087No Hit
GTATCAACGCAGAGTACTTTTTTTT24780.2287108754882488No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21160.1952995207962609No Hit
GAGTACATGGAAGCAGTGGTATCAA19950.18413163704562402No Hit
CATGTACTCTGCGTTGATACCACTG18700.17259456705529672No Hit
TATCAACGCAGAGTACTTTTTTTTT17530.16179586954435035No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15170.14001388140261237No Hit
GCGCAAGACGGACCAGAGCGAAAGC14440.1332762325282612No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14080.12995355637104694No Hit
CCCATGTACTCTGCGTTGATACCAC13780.12718465957336839No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13780.12718465957336839No Hit
GAATAGGACCGCGGTTCTATTTTGT13620.12570791461460648No Hit
GCTTCCATGTACTCTGCGTTGATAC13290.12266212813716007No Hit
GAACTACGACGGTATCTGATCGTCT12680.11703203798188035No Hit
GTACATGGGAAGCAGTGGTATCAAC12470.11509381022350536No Hit
GTCCTATTCCATTATTCCTAGCTGC12310.11361706526474345No Hit
GAATAACGCCGCCGCATCGCCAGTC12070.11140194782660062No Hit
GTATCTGATCGTCTTCGAACCTCCG11690.1078946785495411No Hit
CATGGAAGCAGTGGTATCAACGCAG11640.10743319574992802No Hit
GGGTAGGCACACGCTGAGCCAGTCA11600.10706400951023753No Hit
GATTAAGAGGGACGGCCGGGGGCAT11500.10614104391101135No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11190.10327985055341017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT804.3291948E-1016.6172032
CGAACGA807.683411E-915.44095416
ATCGCCA2450.014.73799116
GCGTTAT851.7273123E-814.52125551
ACCTTAC551.9578723E-413.8193597
CGCCAGT2550.013.78739818
AAGACGG2300.013.6284475
CAAGACG2250.013.5066444
GGGTAGG2350.013.3329351
CGCATCG2650.013.2652813
AGGCGTG801.9958934E-613.0636127
GCATCGC2700.013.01962714
CATCGCC2850.013.00170815
ATAACGA957.365816E-813.00170612
ACGAACG957.365816E-813.00170615
CCGACCA2200.012.9556489
TAGGACA1102.755769E-912.9502634
CGACCAT2150.012.81504410
TCGCCAG2750.012.7858617
GCCTAAG751.48615745E-512.6595561
Produced by FastQC (version 0.11.5)