##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062525_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1083464
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.085010669482326	32.0	32.0	32.0	32.0	32.0
2	30.713889893895875	32.0	32.0	32.0	32.0	32.0
3	30.71833305029055	32.0	32.0	32.0	32.0	32.0
4	30.767268686361522	32.0	32.0	32.0	32.0	32.0
5	30.584534419233126	32.0	32.0	32.0	32.0	32.0
6	34.208376097406095	36.0	36.0	36.0	32.0	36.0
7	34.07074531318069	36.0	36.0	36.0	32.0	36.0
8	34.045401600791536	36.0	36.0	36.0	32.0	36.0
9	34.207715254037055	36.0	36.0	36.0	32.0	36.0
10	33.87220802906234	36.0	36.0	36.0	32.0	36.0
11	34.23999874476679	36.0	36.0	36.0	32.0	36.0
12	33.98610659883485	36.0	36.0	36.0	32.0	36.0
13	34.123290667710236	36.0	36.0	36.0	32.0	36.0
14	34.02663309533127	36.0	36.0	36.0	32.0	36.0
15	33.973476737575034	36.0	36.0	36.0	32.0	36.0
16	33.96854717830957	36.0	36.0	36.0	32.0	36.0
17	33.8816822709384	36.0	36.0	36.0	32.0	36.0
18	33.89957857390739	36.0	36.0	36.0	32.0	36.0
19	33.891563540643716	36.0	36.0	36.0	32.0	36.0
20	33.86684744486203	36.0	36.0	36.0	32.0	36.0
21	33.83805922485657	36.0	36.0	36.0	32.0	36.0
22	33.79241580707804	36.0	36.0	36.0	32.0	36.0
23	33.76292705618276	36.0	36.0	36.0	32.0	36.0
24	33.737692253734316	36.0	36.0	36.0	27.0	36.0
25	33.2646686922685	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	37.0
5	107.0
6	191.0
7	50.0
8	153.0
9	190.0
10	137.0
11	45.0
12	86.0
13	58.0
14	199.0
15	215.0
16	373.0
17	523.0
18	723.0
19	1104.0
20	1787.0
21	2670.0
22	3998.0
23	5790.0
24	7690.0
25	10701.0
26	14485.0
27	18356.0
28	24356.0
29	32284.0
30	41104.0
31	55426.0
32	79186.0
33	111730.0
34	242167.0
35	427537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.07459261679672	17.218736212106947	11.020714838218979	25.685956332877353
2	16.4738990741433	20.07035071056802	37.05475854901429	26.400991666274393
3	19.389746155223563	24.134452261269427	28.089250870855015	28.386550712652
4	12.331947434302108	15.216857504717101	35.838259675138374	36.61293538584241
5	14.338394493506973	36.92074029277481	33.58037457817253	15.160490635545687
6	34.29230730874462	35.0763599495089	17.122560973920624	13.508771767825865
7	30.169993387196282	30.469139633598953	21.042569462146314	18.31829751705845
8	28.545668024946025	32.583000230023735	19.82813809183767	19.04319365319257
9	27.29957770824268	14.337687437582398	18.740570957671974	39.62216389650295
10	15.62736792302887	26.98914775100446	31.91415043080028	25.469333895166386
11	37.617217965351166	21.178703570227285	21.760311669494314	19.44376679492724
12	24.36119480231419	24.019611467440527	28.958429603889023	22.66076412635626
13	29.675723827442052	19.42616830720667	25.30134064707383	25.59676721827745
14	23.349120026614052	20.06154500131684	25.197595494092695	31.39173947797641
15	25.323839523067242	27.242806415159542	22.96671098795194	24.466643073821274
16	25.671375349270413	25.71655874395707	24.49475909581467	24.11730681095785
17	23.71881298278136	26.000031419195835	25.925364624388138	24.355790973634676
18	24.9227627061471	24.491731011824722	27.216453877113455	23.369052404914722
19	25.04944309515624	25.95762495702609	25.350999767109162	23.641932180708505
20	25.02975574415235	24.313677292486027	26.15151033503859	24.505056628323032
21	26.385554193952636	24.707178358276543	24.320600189638633	24.586667258132188
22	24.98844565390204	25.27046413346102	25.14539989129671	24.59569032134023
23	23.566479261420614	24.786846942934595	25.790664982083587	25.856008813561203
24	24.024169452333442	26.039830799046605	25.40565069078683	24.530349057833124
25	24.618158529248404	24.997897526844653	25.33115107401722	25.052792869889718
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	175.0
1	175.0
2	446.5
3	718.0
4	718.0
5	718.0
6	2007.5
7	3297.0
8	3297.0
9	3297.0
10	3341.5
11	3386.0
12	3386.0
13	3386.0
14	3491.0
15	3596.0
16	3596.0
17	3596.0
18	5588.0
19	7580.0
20	7580.0
21	7580.0
22	12187.0
23	16794.0
24	16794.0
25	16794.0
26	24806.0
27	32818.0
28	32818.0
29	32818.0
30	40699.0
31	48580.0
32	48580.0
33	48580.0
34	58624.5
35	68669.0
36	68669.0
37	68669.0
38	77243.5
39	85818.0
40	85818.0
41	85818.0
42	99009.5
43	112201.0
44	112201.0
45	112201.0
46	134705.5
47	157210.0
48	157210.0
49	157210.0
50	151101.0
51	144992.0
52	144992.0
53	144992.0
54	130953.5
55	116915.0
56	116915.0
57	116915.0
58	107809.0
59	98703.0
60	98703.0
61	98703.0
62	87555.0
63	76407.0
64	76407.0
65	76407.0
66	63069.5
67	49732.0
68	49732.0
69	49732.0
70	37367.5
71	25003.0
72	25003.0
73	25003.0
74	19581.0
75	14159.0
76	14159.0
77	14159.0
78	11982.0
79	9805.0
80	9805.0
81	9805.0
82	6764.5
83	3724.0
84	3724.0
85	3724.0
86	2784.5
87	1845.0
88	1845.0
89	1845.0
90	1248.0
91	651.0
92	651.0
93	651.0
94	407.5
95	164.0
96	164.0
97	164.0
98	343.0
99	522.0
100	522.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007937504153345196
2	0.0032303795972916495
3	0.004245641756440453
4	0.01587500830669039
5	0.034980396210672435
6	0.0462405765212319
7	0.06608433690459489
8	0.08925077344517215
9	0.09571153263975546
10	0.11924715542002318
11	0.11924715542002318
12	0.1332762325282612
13	0.11998552789940413
14	0.12266212813716007
15	0.11214032030598155
16	0.11149424438652322
17	0.12210834877762436
18	0.13004585293096954
19	0.13032274261073742
20	0.1218314590978565
21	0.1297689632512017
22	0.14952042707464208
23	0.13798335708431475
24	0.13263015660880287
25	0.12967666669127909
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1083464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.87740956698461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70699053108187	42.944061741948616
2	13.224829191756532	14.250390776353614
3	3.567237591918456	5.765805622876038
4	1.3216473452396396	2.848277412903759
5	0.6090095989430149	1.640592979623893
6	0.3189552373615989	1.0310689174119396
7	0.20096742908017146	0.7579323140323433
8	0.13638945087398666	0.5878648244283126
9	0.09836179613658241	0.47695308985754054
>10	0.6051486119453492	6.434617935728032
>50	0.09534424387324433	3.6754593346691253
>100	0.10014834362215533	11.651909767494088
>500	0.009980418778339132	3.6841175861998914
>1k	0.004990209389169566	4.250947696472832
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4826	0.4454231981865572	No Hit
TCCATGTACTCTGCGTTGATACCAC	4143	0.3823846477594087	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2478	0.2287108754882488	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2116	0.1952995207962609	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1995	0.18413163704562402	No Hit
CATGTACTCTGCGTTGATACCACTG	1870	0.17259456705529672	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1753	0.16179586954435035	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1517	0.14001388140261237	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1444	0.1332762325282612	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1408	0.12995355637104694	No Hit
CCCATGTACTCTGCGTTGATACCAC	1378	0.12718465957336839	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1378	0.12718465957336839	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1362	0.12570791461460648	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1329	0.12266212813716007	No Hit
GAACTACGACGGTATCTGATCGTCT	1268	0.11703203798188035	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1247	0.11509381022350536	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1231	0.11361706526474345	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1207	0.11140194782660062	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1169	0.1078946785495411	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1164	0.10743319574992802	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1160	0.10706400951023753	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1150	0.10614104391101135	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1119	0.10327985055341017	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.229655992261856E-5
2	0.0	1.8459311984523713E-4	0.0	9.229655992261856E-5	9.229655992261856E-5
3	0.0	1.8459311984523713E-4	0.0	9.229655992261856E-5	9.229655992261856E-5
4	0.0	1.8459311984523713E-4	0.0	1.8459311984523713E-4	9.229655992261856E-5
5	0.0	2.768896797678557E-4	0.0	2.768896797678557E-4	9.229655992261856E-5
6	0.0	2.768896797678557E-4	0.0	2.768896797678557E-4	9.229655992261856E-5
7	0.0	2.768896797678557E-4	0.0	2.768896797678557E-4	9.229655992261856E-5
8	0.0	2.768896797678557E-4	0.0	2.768896797678557E-4	9.229655992261856E-5
9	0.0	2.768896797678557E-4	0.0	2.768896797678557E-4	9.229655992261856E-5
10	9.229655992261856E-5	2.768896797678557E-4	0.0	2.768896797678557E-4	9.229655992261856E-5
11	9.229655992261856E-5	2.768896797678557E-4	0.0	3.6918623969047425E-4	9.229655992261856E-5
12	9.229655992261856E-5	2.768896797678557E-4	0.0	3.6918623969047425E-4	2.768896797678557E-4
13	9.229655992261856E-5	2.768896797678557E-4	0.0	3.6918623969047425E-4	3.6918623969047425E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	80	4.3291948E-10	16.617203	2
CGAACGA	80	7.683411E-9	15.440954	16
ATCGCCA	245	0.0	14.737991	16
GCGTTAT	85	1.7273123E-8	14.5212555	1
ACCTTAC	55	1.9578723E-4	13.819359	7
CGCCAGT	255	0.0	13.787398	18
AAGACGG	230	0.0	13.628447	5
CAAGACG	225	0.0	13.506644	4
GGGTAGG	235	0.0	13.332935	1
CGCATCG	265	0.0	13.26528	13
AGGCGTG	80	1.9958934E-6	13.063612	7
GCATCGC	270	0.0	13.019627	14
CATCGCC	285	0.0	13.001708	15
ATAACGA	95	7.365816E-8	13.001706	12
ACGAACG	95	7.365816E-8	13.001706	15
CCGACCA	220	0.0	12.955648	9
TAGGACA	110	2.755769E-9	12.950263	4
CGACCAT	215	0.0	12.815044	10
TCGCCAG	275	0.0	12.78586	17
GCCTAAG	75	1.48615745E-5	12.659556	1
>>END_MODULE