FastQCFastQC Report
Thu 2 Feb 2017
SRR4062525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083464
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA28330.2614761542607784No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC22540.20803644606558225No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14490.13373771532787432No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13930.12856910797220766No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13680.1262616939741422No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13600.12552332149476125No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12780.11795500358110651No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12120.1118634306262137No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11320.10447970583240421No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC11210.10346444367325541No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC11210.10346444367325541No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG11020.10171080903472567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG450.001398205624.4441917
TAGGACG2750.020.8007414
GTAAACG2900.018.96531727
ACTAGAC1051.7917264E-618.8578153
CGTACAC708.1206334E-418.8578153
AGGACGT2850.018.5269765
TCCTACA4750.018.5269762
ATTATCC1203.1600212E-718.3339883
CGCAATA3500.018.2283836
TAAACGC3150.018.15853928
AACGCTT3150.018.15853930
GTCCTAC4250.018.121641
TAATACT2550.018.1182924
AAACGCT3050.018.03259829
ATACCGT2950.017.8989436
CTAGCGG3700.017.83765229
GTCCTAA2100.017.8134481
ACCGTCG2900.017.4480928
CGTCGTA3050.017.31129510
CCGTCGT2950.017.1523639
Produced by FastQC (version 0.11.5)