##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062525_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1083464
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281862618416486	32.0	32.0	32.0	32.0	32.0
2	31.431355356523152	32.0	32.0	32.0	32.0	32.0
3	31.518966943064097	32.0	32.0	32.0	32.0	32.0
4	31.621750238125124	32.0	32.0	32.0	32.0	32.0
5	31.564416538066794	32.0	32.0	32.0	32.0	32.0
6	35.16906606956945	36.0	36.0	36.0	36.0	36.0
7	35.17645902401926	36.0	36.0	36.0	36.0	36.0
8	35.11172498578633	36.0	36.0	36.0	36.0	36.0
9	35.22737442130057	36.0	36.0	36.0	36.0	36.0
10	35.12359801525478	36.0	36.0	36.0	36.0	36.0
11	35.24772765869471	36.0	36.0	36.0	36.0	36.0
12	35.147898776516804	36.0	36.0	36.0	36.0	36.0
13	35.19340374945545	36.0	36.0	36.0	36.0	36.0
14	35.155957189163644	36.0	36.0	36.0	36.0	36.0
15	35.12923456616925	36.0	36.0	36.0	36.0	36.0
16	35.136686590417405	36.0	36.0	36.0	36.0	36.0
17	35.111537623769685	36.0	36.0	36.0	36.0	36.0
18	35.12195144462576	36.0	36.0	36.0	36.0	36.0
19	35.09890591657868	36.0	36.0	36.0	36.0	36.0
20	35.083902187797655	36.0	36.0	36.0	36.0	36.0
21	35.06843513028583	36.0	36.0	36.0	36.0	36.0
22	35.053546772204704	36.0	36.0	36.0	36.0	36.0
23	35.021341733550905	36.0	36.0	36.0	36.0	36.0
24	34.99429422666558	36.0	36.0	36.0	36.0	36.0
25	34.964168629506844	36.0	36.0	36.0	36.0	36.0
26	34.91553572615241	36.0	36.0	36.0	32.0	36.0
27	34.90259297955446	36.0	36.0	36.0	32.0	36.0
28	34.87645182488758	36.0	36.0	36.0	32.0	36.0
29	34.84812231878493	36.0	36.0	36.0	32.0	36.0
30	34.84036110106104	36.0	36.0	36.0	32.0	36.0
31	34.82316809787866	36.0	36.0	36.0	32.0	36.0
32	34.78544926273508	36.0	36.0	36.0	32.0	36.0
33	34.75873679236228	36.0	36.0	36.0	32.0	36.0
34	34.73606321945169	36.0	36.0	36.0	32.0	36.0
35	34.68042685313033	36.0	36.0	36.0	32.0	36.0
36	34.651413429518655	36.0	36.0	36.0	32.0	36.0
37	34.62634752977487	36.0	36.0	36.0	32.0	36.0
38	34.573339769480114	36.0	36.0	36.0	32.0	36.0
39	34.5318524657949	36.0	36.0	36.0	32.0	36.0
40	34.49279348460124	36.0	36.0	36.0	32.0	36.0
41	34.46419262661242	36.0	36.0	36.0	32.0	36.0
42	34.40592673129887	36.0	36.0	36.0	32.0	36.0
43	34.39224930408394	36.0	36.0	36.0	32.0	36.0
44	34.35934742640272	36.0	36.0	36.0	32.0	36.0
45	34.27473916992166	36.0	36.0	36.0	32.0	36.0
46	34.29660330200173	36.0	36.0	36.0	32.0	36.0
47	34.25569100588483	36.0	36.0	36.0	32.0	36.0
48	34.21042508103638	36.0	36.0	36.0	32.0	36.0
49	34.19734481256415	36.0	36.0	36.0	32.0	36.0
50	33.664738283874684	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	19.0
22	100.0
23	264.0
24	760.0
25	1747.0
26	3600.0
27	6895.0
28	11763.0
29	18223.0
30	25699.0
31	36720.0
32	54080.0
33	84971.0
34	183058.0
35	655556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.73823891324355	17.701424447773785	11.526940442002516	26.033396196980146
2	15.943652923867576	20.530667473980735	36.73617443891057	26.789505163241117
3	18.694587145971507	24.414328935685557	28.5289323628604	28.36215155548254
4	12.039358981340387	15.682953640318129	36.129614024659816	36.14807335368167
5	13.981175193638181	37.1425354234197	33.64357283675323	15.23271654618889
6	34.52304477523776	35.24444001417686	16.760071474983757	13.47244373560163
7	30.114890757791674	30.521641697370654	21.038354758441443	18.325112786396225
8	28.563293288932535	32.71829982352898	19.69110187325098	19.027305014287506
9	27.463672074014454	14.033322749994461	18.708143510075093	39.79486166591599
10	15.713027844026199	26.926321502144972	32.04490412233355	25.315746531495275
11	37.821561214770405	20.954272592351938	21.767589878390055	19.456576314487606
12	24.386135579954665	24.103154327231916	29.008624190559168	22.50208590225425
13	29.859690769605635	19.271152525603068	25.39161430375167	25.477542401039628
14	23.33072441723952	19.940487178161895	25.318423131733038	31.410365272865548
15	25.368909350010703	27.19213559472211	22.977413185855735	24.461541869411445
16	25.77547569646984	25.62410933819675	24.42933036999845	24.171084595334964
17	23.763687579836525	26.016277421307954	25.901368204204296	24.318666794651232
18	25.123585093736384	24.185298265563045	27.324304268531304	23.366812372169264
19	25.091465890883313	25.773445172151543	25.555994476973854	23.579094459991286
20	25.228710875488247	24.06383599270488	26.26815473333678	24.43929839847009
21	26.380202757082838	24.6502883344532	24.498645086500336	24.47086382196363
22	24.995108282324104	24.996400434163018	25.33411354691988	24.674377736593
23	23.48144469959316	24.527349316636272	26.017015793787333	25.974190189983236
24	24.08081855973064	25.88826209269528	25.613495233805644	24.417424113768433
25	24.74304637717543	24.65139589317227	25.502831658458426	25.102726071193874
26	23.79811419668766	25.56633169168519	26.208715748746613	24.426838362880538
27	25.364109928894727	25.11980093477956	25.248554635871614	24.2675345004541
28	24.06808163446132	24.63090605686945	26.28366055540378	25.017351753265455
29	23.85100012552332	25.303194199345803	26.41453707737405	24.431268597756826
30	24.305652979702142	25.20332932150953	26.64592455310006	23.845093145688274
31	24.458126896694306	25.15321228947155	25.275782121048785	25.112878692785362
32	24.02368698913854	25.306239985823247	25.44948424682315	25.22058877821506
33	23.717262410195445	24.754583447165757	26.09712920780017	25.431024934838632
34	24.459142158853457	25.284734887361278	26.302581350187914	23.95354160359735
35	25.580268472233502	24.86257042227522	25.903952507882128	23.653208597609147
36	24.183729224044363	25.567254657284415	25.656413134169664	24.59260298450156
37	25.22234241285359	25.49240214718717	25.42382580316466	23.861429636794576
38	24.231077359284665	25.244308994115173	25.627062828114273	24.897550818485893
39	24.716926450717327	25.155796593149383	25.357926059379913	24.769350896753377
40	25.338636078356085	25.16751825625955	25.543903627623994	23.94994203776037
41	23.78999209941447	25.52978225395583	26.33626959455967	24.343956052070027
42	25.745663907614833	25.715390635960215	25.47025097280574	23.068694483619208
43	24.60764732376895	24.360661729416023	25.695916061816543	25.335774884998486
44	24.160193601264094	25.243755214755637	25.80648734060384	24.78956384337643
45	24.46947937356479	25.511692128211	25.507169596774787	24.511658901449426
46	23.904277303424298	24.985624797524235	26.04417502028219	25.065922878769282
47	24.631459836228984	24.650103741333353	26.368850280212357	24.349586142225306
48	25.07300657889879	26.187026057164797	24.74895335701048	23.991014006925933
49	23.873335893024596	25.527197950277998	25.908567335878256	24.69089882081915
50	23.738398322417726	26.674259596996304	24.71000420872313	24.87733787186284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	18.5
2	20.0
3	78.5
4	137.0
5	177.5
6	218.0
7	195.0
8	172.0
9	249.5
10	327.0
11	513.5
12	700.0
13	1243.0
14	1786.0
15	2386.5
16	2987.0
17	3652.0
18	4317.0
19	4591.0
20	4865.0
21	5494.0
22	6123.0
23	6587.0
24	7051.0
25	7569.0
26	8087.0
27	9797.5
28	11508.0
29	13233.0
30	14958.0
31	16684.0
32	18410.0
33	21303.5
34	24197.0
35	27753.0
36	31309.0
37	36880.5
38	42452.0
39	45040.5
40	47629.0
41	51846.0
42	56063.0
43	57603.0
44	59143.0
45	67442.5
46	75742.0
47	82352.0
48	88962.0
49	88486.0
50	88010.0
51	81105.0
52	74200.0
53	71641.0
54	69082.0
55	70913.0
56	72744.0
57	70146.0
58	67548.0
59	61893.5
60	56239.0
61	48874.0
62	41509.0
63	36207.0
64	30905.0
65	26632.5
66	22360.0
67	19344.5
68	16329.0
69	15215.0
70	14101.0
71	11007.5
72	7914.0
73	7178.0
74	6442.0
75	4496.0
76	2550.0
77	2379.5
78	2209.0
79	1925.0
80	1641.0
81	1309.5
82	978.0
83	829.5
84	681.0
85	600.5
86	520.0
87	374.5
88	229.0
89	142.5
90	56.0
91	36.0
92	16.0
93	13.5
94	11.0
95	6.5
96	2.0
97	4.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015413525507077301
2	0.003322676157214268
3	5.537793595357113E-4
4	9.229655992261856E-5
5	0.0
6	7.383724793809485E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.229655992261856E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1083464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.07642276294407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.08233307761802	45.708130985810264
2	13.041382958359849	14.887109742896017
3	3.537593399707547	6.057395293353256
4	1.2643356975371671	2.886550351476526
5	0.5691367187542046	1.6242143984764887
6	0.3192390596650129	1.0932614119131003
7	0.19917580565850432	0.7957769741540346
8	0.14262746931059422	0.6512532588784246
9	0.10378516908622888	0.533131756856031
>10	0.5582015037215197	6.270535403083192
>50	0.08208586348597183	3.3129433486268156
>100	0.08807788435178582	10.516896016735664
>500	0.009262802518828862	3.5517606162521855
>1k	0.0027625902249138715	2.1110404414879937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2833	0.2614761542607784	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2254	0.20803644606558225	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1449	0.13373771532787432	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1393	0.12856910797220766	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1368	0.1262616939741422	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1360	0.12552332149476125	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1278	0.11795500358110651	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1212	0.1118634306262137	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1132	0.10447970583240421	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1121	0.10346444367325541	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1121	0.10346444367325541	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1102	0.10171080903472567	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.229655992261856E-5	0.0
5	0.0	9.229655992261856E-5	0.0	1.8459311984523713E-4	0.0
6	0.0	9.229655992261856E-5	0.0	1.8459311984523713E-4	0.0
7	0.0	9.229655992261856E-5	0.0	2.768896797678557E-4	0.0
8	0.0	9.229655992261856E-5	0.0	2.768896797678557E-4	0.0
9	0.0	9.229655992261856E-5	0.0	2.768896797678557E-4	0.0
10	0.0	9.229655992261856E-5	0.0	3.6918623969047425E-4	0.0
11	0.0	9.229655992261856E-5	0.0	4.6148279961309285E-4	0.0
12	0.0	9.229655992261856E-5	0.0	4.6148279961309285E-4	3.6918623969047425E-4
13	0.0	9.229655992261856E-5	0.0	4.6148279961309285E-4	4.6148279961309285E-4
14	0.0	9.229655992261856E-5	0.0	4.6148279961309285E-4	4.6148279961309285E-4
15	0.0	9.229655992261856E-5	0.0	4.6148279961309285E-4	5.537793595357114E-4
16	0.0	9.229655992261856E-5	0.0	6.4607591945833E-4	5.537793595357114E-4
17	0.0	9.229655992261856E-5	0.0	7.383724793809485E-4	5.537793595357114E-4
18	0.0	9.229655992261856E-5	0.0	7.383724793809485E-4	5.537793595357114E-4
19	0.0	9.229655992261856E-5	0.0	7.383724793809485E-4	5.537793595357114E-4
20	0.0	9.229655992261856E-5	0.0	0.0010152621591488042	5.537793595357114E-4
21	0.0	9.229655992261856E-5	0.0	0.0013844483988392784	5.537793595357114E-4
22	0.0	9.229655992261856E-5	0.0	0.002122820878220227	5.537793595357114E-4
23	0.0	9.229655992261856E-5	0.0	0.0035072692770595055	5.537793595357114E-4
24	0.0	9.229655992261856E-5	0.0	0.005445497035434495	5.537793595357114E-4
25	0.0	9.229655992261856E-5	0.0	0.006829945434273774	5.537793595357114E-4
26	0.0	9.229655992261856E-5	0.0	0.008491283512880908	5.537793595357114E-4
27	0.0	9.229655992261856E-5	0.0	0.01033721471133328	6.4607591945833E-4
28	0.0	9.229655992261856E-5	0.0	0.015136635827309444	6.4607591945833E-4
29	0.0	9.229655992261856E-5	0.0	0.02270495374096417	6.4607591945833E-4
30	0.0	9.229655992261856E-5	0.0	0.039133741407190274	6.4607591945833E-4
31	0.0	9.229655992261856E-5	0.0	0.07226820641941034	7.383724793809485E-4
32	0.0	9.229655992261856E-5	0.0	0.11075587190714228	7.383724793809485E-4
33	0.0	9.229655992261856E-5	0.0	0.1525662135520885	7.383724793809485E-4
34	0.0	9.229655992261856E-5	0.0	0.20046812815192752	7.383724793809485E-4
35	0.0	9.229655992261856E-5	0.0	0.2599071127420939	7.383724793809485E-4
36	0.0	9.229655992261856E-5	0.0	0.3420510510732244	7.383724793809485E-4
37	0.0	9.229655992261856E-5	0.0	0.4593599787348726	7.383724793809485E-4
38	0.0	9.229655992261856E-5	0.0	0.6123876750865742	7.383724793809485E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGACG	45	0.0013982056	24.44419	17
TAGGACG	275	0.0	20.800741	4
GTAAACG	290	0.0	18.965317	27
ACTAGAC	105	1.7917264E-6	18.857815	3
CGTACAC	70	8.1206334E-4	18.857815	3
AGGACGT	285	0.0	18.526976	5
TCCTACA	475	0.0	18.526976	2
ATTATCC	120	3.1600212E-7	18.333988	3
CGCAATA	350	0.0	18.22838	36
TAAACGC	315	0.0	18.158539	28
AACGCTT	315	0.0	18.158539	30
GTCCTAC	425	0.0	18.12164	1
TAATACT	255	0.0	18.118292	4
AAACGCT	305	0.0	18.032598	29
ATACCGT	295	0.0	17.898943	6
CTAGCGG	370	0.0	17.837652	29
GTCCTAA	210	0.0	17.813448	1
ACCGTCG	290	0.0	17.448092	8
CGTCGTA	305	0.0	17.311295	10
CCGTCGT	295	0.0	17.152363	9
>>END_MODULE