FastQCFastQC Report
Thu 2 Feb 2017
SRR4062524_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062524_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165950
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG57150.49015824006175224No Hit
TCCATGTACTCTGCGTTGATACCAC46460.39847334791371847No Hit
GTATCAACGCAGAGTACTTTTTTTT33380.28629014966336463No Hit
GAGTACATGGAAGCAGTGGTATCAA24880.21338822419486259No Hit
TATCAACGCAGAGTACTTTTTTTTT22400.19211801535228784No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22270.19100304472747545No Hit
CATGTACTCTGCGTTGATACCACTG20910.17933873665251512No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17090.1465757536772589No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16970.1455465500235859No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16160.13859942536129335No Hit
GAATAGGACCGCGGTTCTATTTTGT16120.1382563574767357No Hit
GCGCAAGACGGACCAGAGCGAAAGC16080.13791328959217805No Hit
GCTTCCATGTACTCTGCGTTGATAC15720.13482567863115916No Hit
GAATAACGCCGCCGCATCGCCAGTC15590.13371070800634674No Hit
GGGTAGGCACACGCTGAGCCAGTCA14880.12762125305544836No Hit
GATTAAGAGGGACGGCCGGGGGCAT14560.12487670997898709No Hit
GTATCTGATCGTCTTCGAACCTCCG14410.12359020541189587No Hit
GTACATGGGAAGCAGTGGTATCAAC14330.12290406964278057No Hit
CCCATGTACTCTGCGTTGATACCAC14110.12101719627771346No Hit
GAACTACGACGGTATCTGATCGTCT13760.11801535228783396No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13040.11184013036579614No Hit
GCGTTGATACCACTGCTTCCATGTA13010.1115828294523779No Hit
GTCCTATTCCATTATTCCTAGCTGC12930.11089669368326256No Hit
CATGGAAGCAGTGGTATCAACGCAG12630.10832368454908015No Hit
CTATTGGAGCTGGAATTACCGCGGC12490.10712294695312835No Hit
TCGTAGTTCCGACCATAAACGATGC12060.10343496719413355No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG11980.10274883142501823No Hit
GGTATCAACGCAGAGTACTTTTTTT11910.10214846262704233No Hit
GTGCATGGCCGTTCTTAGTTGGTGG11830.101462326857927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG250.00603307918.998975
GCGCTAT651.9123217E-716.07674211
CGTTATT1300.015.3387372
GCTAGAC456.776532E-414.773173
CGGTCGA653.3677625E-614.61647510
AGGATCG1152.5465852E-1114.0427175
TAGACAC551.9603901E-413.8174325
CGTCTAT707.3048577E-613.5631221
GCGTTAT1351.8189894E-1213.3621871
TGCGAGT500.001498311113.30099213
TGCGTAC500.001499281113.2998511
CGAACGA1153.7471182E-1013.2200816
TAACGAA1153.765308E-1013.21837813
GCTATTA655.4463846E-513.1548289
GGACAAT655.4485798E-513.15426356
GTTATTC1450.013.0993633
CCAACGA957.3609044E-813.00264519
TAGAGAG957.385643E-812.9992955
CGGTCCA2500.012.92096310
AGGCCCG3150.012.66761210
Produced by FastQC (version 0.11.5)