FastQCFastQC Report
Thu 2 Feb 2017
SRR4062524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165950
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20920.17942450362365453No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC19750.16938976800034308No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18190.15601012050259444No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16410.14074359963977873No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15930.13662678502508685No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15760.1351687465157168No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14490.12427634118101119No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG14280.1224752347870835No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14220.121960632960247No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA13550.11621424589390626No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC12970.11123976156782024No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12700.10892405334705604No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12420.10652257815515247No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12180.10446417084780651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG551.5936655E-423.99979214
ATACGAA5750.020.66068840
CGCAATA6000.020.53315436
ATTGTCG1401.8553692E-1020.42839243
TTAGGAC3350.020.358033
TATGGCG5200.020.30751416
CGAATGC5850.020.30751243
TAGGACG3600.020.166494
GTCCTAC4500.020.0502871
TAGTACG1101.2645069E-720.0015412
TTAACGG1001.1234606E-619.79982635
TAGCGGC6300.019.2061830
TCCTACA5100.018.9818532
GCAATAC6500.018.9536837
TAGCGAC1051.7927268E-618.8569779
TGGCGAG5600.018.85697718
GTAGCGA1051.7927268E-618.8569778
TACGAAT6300.018.85697741
CAATACG6450.018.75952538
ATTTCGT1658.185452E-1118.66650242
Produced by FastQC (version 0.11.5)