##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062524_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1165950
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210835799133754	32.0	32.0	32.0	32.0	32.0
2	31.387827951455893	32.0	32.0	32.0	32.0	32.0
3	31.489416355761396	32.0	32.0	32.0	32.0	32.0
4	31.602418628586133	32.0	32.0	32.0	32.0	32.0
5	31.51560701573824	32.0	32.0	32.0	32.0	32.0
6	35.12138084823535	36.0	36.0	36.0	36.0	36.0
7	35.13479222951241	36.0	36.0	36.0	36.0	36.0
8	35.07637634546936	36.0	36.0	36.0	36.0	36.0
9	35.209242248809986	36.0	36.0	36.0	36.0	36.0
10	35.07928641880012	36.0	36.0	36.0	36.0	36.0
11	35.2155589862344	36.0	36.0	36.0	36.0	36.0
12	35.11201080663837	36.0	36.0	36.0	36.0	36.0
13	35.15280329345169	36.0	36.0	36.0	36.0	36.0
14	35.11271581114113	36.0	36.0	36.0	36.0	36.0
15	35.07850422402333	36.0	36.0	36.0	36.0	36.0
16	35.083031004760066	36.0	36.0	36.0	36.0	36.0
17	35.05155967237017	36.0	36.0	36.0	36.0	36.0
18	35.068229340880826	36.0	36.0	36.0	36.0	36.0
19	35.044320940006	36.0	36.0	36.0	36.0	36.0
20	35.03331189159055	36.0	36.0	36.0	36.0	36.0
21	35.01681118401304	36.0	36.0	36.0	36.0	36.0
22	34.995354003173375	36.0	36.0	36.0	36.0	36.0
23	34.94671898451906	36.0	36.0	36.0	32.0	36.0
24	34.917940735022945	36.0	36.0	36.0	32.0	36.0
25	34.88422230798919	36.0	36.0	36.0	32.0	36.0
26	34.84215017796647	36.0	36.0	36.0	32.0	36.0
27	34.81427762768558	36.0	36.0	36.0	32.0	36.0
28	34.78386380204983	36.0	36.0	36.0	32.0	36.0
29	34.753301599554014	36.0	36.0	36.0	32.0	36.0
30	34.7318933058879	36.0	36.0	36.0	32.0	36.0
31	34.727182126163214	36.0	36.0	36.0	32.0	36.0
32	34.68161928041511	36.0	36.0	36.0	32.0	36.0
33	34.660962305416184	36.0	36.0	36.0	32.0	36.0
34	34.62815729662507	36.0	36.0	36.0	32.0	36.0
35	34.57755735666195	36.0	36.0	36.0	32.0	36.0
36	34.5470157382392	36.0	36.0	36.0	32.0	36.0
37	34.50962219649213	36.0	36.0	36.0	32.0	36.0
38	34.460118358420175	36.0	36.0	36.0	32.0	36.0
39	34.39481967494318	36.0	36.0	36.0	32.0	36.0
40	34.360348213902824	36.0	36.0	36.0	32.0	36.0
41	34.33611818688623	36.0	36.0	36.0	32.0	36.0
42	34.2556644796089	36.0	36.0	36.0	32.0	36.0
43	34.26800034306788	36.0	36.0	36.0	32.0	36.0
44	34.218223766027705	36.0	36.0	36.0	32.0	36.0
45	34.11029375187615	36.0	36.0	36.0	32.0	36.0
46	34.163621939191216	36.0	36.0	36.0	32.0	36.0
47	34.09208713924268	36.0	36.0	36.0	32.0	36.0
48	34.06732707234444	36.0	36.0	36.0	32.0	36.0
49	34.07021570393242	36.0	36.0	36.0	32.0	36.0
50	33.53636605343282	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	3.0
21	26.0
22	106.0
23	326.0
24	878.0
25	2231.0
26	4722.0
27	9120.0
28	14720.0
29	21116.0
30	29863.0
31	41828.0
32	61580.0
33	94592.0
34	205579.0
35	679255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05865146660833	17.36023366229622	11.478445854081155	26.102669017014286
2	16.185806991692242	20.154164429478392	36.52583673700443	27.13419184182493
3	19.085838673074072	23.816601669206815	28.423430647981935	28.674129009737182
4	12.31434651086797	15.138741059857677	35.274956280248965	37.271956149025385
5	14.43982541260381	36.68573839850628	33.01722459558694	15.857211593302967
6	35.19434004662308	34.71515833494861	16.358905129397073	13.731596489031226
7	30.707148677044472	30.14554655002359	20.29306574038338	18.854239032548563
8	28.967047443756115	32.33314664706604	19.2759717620582	19.423834147119642
9	27.633603499292427	13.729662506968566	18.242892062266822	40.39384193147219
10	16.242634761353404	26.058836142201635	30.800120073759597	26.898409022685364
11	38.30575925211201	20.533470560487157	21.43196535014366	19.728804837257172
12	24.742827737038468	23.335820575496378	28.285003645096275	23.636348042368883
13	30.224537930442985	18.584587675286247	25.41412582014666	25.776748574124102
14	24.042197349800592	19.248080964020755	24.394184999356746	32.31553668682191
15	25.936275140443417	26.58072816158497	22.427805652043396	25.055191045928215
16	26.44873279300142	25.169003816630216	23.76431236330889	24.61795102705948
17	24.27085209485827	25.46172648912904	25.36223680260732	24.90518461340538
18	25.74227025172606	23.637119945109138	26.848749946395646	23.771859856769158
19	25.907972039967408	24.96968137570222	24.848664179424503	24.273682404905873
20	25.873150649684806	23.331360692997126	25.541404005317553	25.254084652000515
21	27.06128050087911	23.766199236673955	23.86371628285947	25.308803979587463
22	25.836699686950553	24.200694712466227	24.69273982589305	25.269865774690164
23	23.994768214760494	23.712423345769544	25.515073545177753	26.77773489429221
24	24.757665423045587	25.204339808739658	24.818988807410268	25.21900596080449
25	25.375016081307088	23.95325700072902	24.769758565976243	25.901968351987648
26	24.26536300870535	24.931686607487457	25.697499892791285	25.105450491015908
27	25.739783009563016	24.527295338565118	24.923109910373515	24.80981174149835
28	24.523693125777264	23.946996011835843	25.621510356361764	25.90780050602513
29	24.184313220978602	24.59891075946653	25.789613619795016	25.42716239975985
30	24.66649513272439	24.55774261331961	25.96483554183284	24.81092671212316
31	25.0399245250654	24.453192675500667	24.53003988164158	25.976842917792357
32	24.89875209056992	24.531926755006648	24.751833269008106	25.817487885415325
33	24.42934945752391	24.023843217976758	25.532226939405632	26.0145803850937
34	24.9398344697457	24.630301470903554	25.779750418113984	24.65011364123676
35	26.066469402633047	24.215532398473346	25.392512543419528	24.325485655474075
36	24.43483854367683	25.15562416913247	25.08066383635662	25.328873450834084
37	26.082593593207253	24.715125005360434	24.637334362536986	24.564947038895323
38	25.03486427376817	24.39341309661649	24.84489043269437	25.726832196920967
39	25.52399331017625	24.05454779364467	24.835284531926753	25.58617436425233
40	25.855396886658948	24.51674600111497	24.955872893348772	24.67198421887731
41	24.27068056091599	24.90183970153094	25.942364595394313	24.885115142158753
42	26.501479480252154	25.07011449890647	24.968309104163986	23.460096916677387
43	25.183584201723917	23.612676358334404	25.07654702174193	26.12719241819975
44	24.606029418071103	24.54453449976414	25.34251039924525	25.50692568291951
45	24.924825249796303	24.90046742999271	24.864445302114156	25.31026201809683
46	24.44641708478065	24.37197135383164	25.39011106822763	25.791500493160086
47	25.103392083708563	24.079934817101933	25.858913332475662	24.95775976671384
48	25.702903211973073	25.558385865603157	23.94433723573052	24.794373686693255
49	24.365367297053904	25.072601741069516	25.356576182512114	25.205454779364466
50	24.255242506110896	26.087653844504484	24.07015738239204	25.586946266992584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	18.5
2	30.0
3	86.5
4	143.0
5	183.0
6	223.0
7	209.0
8	195.0
9	270.5
10	346.0
11	529.0
12	712.0
13	1221.5
14	1731.0
15	2405.5
16	3080.0
17	3586.0
18	4092.0
19	4462.0
20	4832.0
21	5178.0
22	5524.0
23	5856.0
24	6188.0
25	6251.0
26	6314.0
27	7970.5
28	9627.0
29	11282.0
30	12937.0
31	14524.5
32	16112.0
33	19101.5
34	22091.0
35	25639.5
36	29188.0
37	34563.0
38	39938.0
39	43147.5
40	46357.0
41	50902.5
42	55448.0
43	57534.0
44	59620.0
45	68322.5
46	77025.0
47	84087.5
48	91150.0
49	93034.0
50	94918.0
51	88197.0
52	81476.0
53	79118.0
54	76760.0
55	79200.5
56	81641.0
57	80133.5
58	78626.0
59	72820.0
60	67014.0
61	59317.5
62	51621.0
63	45238.5
64	38856.0
65	33750.5
66	28645.0
67	25276.0
68	21907.0
69	20870.5
70	19834.0
71	15304.5
72	10775.0
73	9691.5
74	8608.0
75	6267.5
76	3927.0
77	3495.5
78	3064.0
79	2621.5
80	2179.0
81	1702.0
82	1225.0
83	1071.0
84	917.0
85	767.0
86	617.0
87	453.0
88	289.0
89	187.5
90	86.0
91	58.5
92	31.0
93	23.0
94	15.0
95	10.5
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014151550238003345
2	0.0044598824992495385
3	6.003687979758995E-4
4	8.576697113941421E-5
5	8.576697113941421E-5
6	3.4306788455765684E-4
7	0.0
8	8.576697113941421E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1165950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.204636974363524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.81464598429699	35.02988195044329
2	13.520023432229802	12.493755491421325
3	4.3396420838624685	6.0153476125060745
4	1.8939542210037228	3.500378689101619
5	1.0551680749498547	2.4376828924998017
6	0.6717990837330916	1.8624139670158524
7	0.4532501880099128	1.4659582276892023
8	0.33407377464479454	1.234860598409445
9	0.24089843596094254	1.0017562303140577
>10	1.3996762443082873	12.087297771627352
>50	0.13265590539658637	4.262740645257864
>100	0.12740022598201553	12.040615710502916
>500	0.012889464976571037	4.070907189124515
>1k	0.003922880645043359	2.496403024086682
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2092	0.17942450362365453	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1975	0.16938976800034308	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1819	0.15601012050259444	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1641	0.14074359963977873	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1593	0.13662678502508685	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1576	0.1351687465157168	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1449	0.12427634118101119	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1428	0.1224752347870835	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1422	0.121960632960247	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1355	0.11621424589390626	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1297	0.11123976156782024	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1270	0.10892405334705604	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1242	0.10652257815515247	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1218	0.10446417084780651	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	8.576697113941421E-5	0.0
12	0.0	0.0	0.0	8.576697113941421E-5	0.0
13	0.0	0.0	0.0	8.576697113941421E-5	0.0
14	0.0	0.0	0.0	8.576697113941421E-5	0.0
15	0.0	0.0	0.0	8.576697113941421E-5	0.0
16	0.0	0.0	0.0	8.576697113941421E-5	0.0
17	0.0	0.0	0.0	4.2883485569707104E-4	0.0
18	0.0	0.0	0.0	5.146018268364853E-4	0.0
19	0.0	0.0	0.0	6.003687979758994E-4	0.0
20	0.0	0.0	0.0	6.003687979758994E-4	0.0
21	0.0	0.0	0.0	0.0012007375959517989	0.0
22	0.0	0.0	0.0	0.0023157082207641835	0.0
23	0.0	0.0	0.0	0.004031047643552468	0.0
24	0.0	0.0	0.0	0.007204425575710794	0.0
25	0.0	0.0	0.0	0.009005531969638492	0.0
26	0.0	0.0	0.0	0.011321240190402676	0.0
27	0.0	0.0	0.0	0.014494618122561002	0.0
28	0.0	0.0	0.0	0.02238517946738711	0.0
29	0.0	0.0	0.0	0.03370641965778978	0.0
30	0.0	0.0	0.0	0.056434667009734554	0.0
31	8.576697113941421E-5	0.0	0.0	0.10394956902097002	0.0
32	8.576697113941421E-5	0.0	0.0	0.15601012050259444	0.0
33	8.576697113941421E-5	0.0	0.0	0.2051545949654788	8.576697113941421E-5
34	8.576697113941421E-5	0.0	0.0	0.2695655902911789	8.576697113941421E-5
35	8.576697113941421E-5	0.0	0.0	0.33903683691410436	8.576697113941421E-5
36	8.576697113941421E-5	0.0	0.0	0.44384407564646855	8.576697113941421E-5
37	8.576697113941421E-5	0.0	0.0	0.5840730734594107	8.576697113941421E-5
38	8.576697113941421E-5	0.0	0.0	0.7616964706891376	8.576697113941421E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	55	1.5936655E-4	23.999792	14
ATACGAA	575	0.0	20.660688	40
CGCAATA	600	0.0	20.533154	36
ATTGTCG	140	1.8553692E-10	20.428392	43
TTAGGAC	335	0.0	20.35803	3
TATGGCG	520	0.0	20.307514	16
CGAATGC	585	0.0	20.307512	43
TAGGACG	360	0.0	20.16649	4
GTCCTAC	450	0.0	20.050287	1
TAGTACG	110	1.2645069E-7	20.001541	2
TTAACGG	100	1.1234606E-6	19.799826	35
TAGCGGC	630	0.0	19.20618	30
TCCTACA	510	0.0	18.981853	2
GCAATAC	650	0.0	18.95368	37
TAGCGAC	105	1.7927268E-6	18.856977	9
TGGCGAG	560	0.0	18.856977	18
GTAGCGA	105	1.7927268E-6	18.856977	8
TACGAAT	630	0.0	18.856977	41
CAATACG	645	0.0	18.759525	38
ATTTCGT	165	8.185452E-11	18.666502	42
>>END_MODULE