FastQCFastQC Report
Thu 2 Feb 2017
SRR4062523_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062523_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4267026
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88540.20749814976519948No Hit
TATCAACGCAGAGTACTTTTTTTTT52030.12193504328307352No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43250.10135865120109416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2650.013.612671
CGAACGA2500.012.92236216
CGTTATT2800.012.8855352
TATTCCG853.948604E-612.2939125
AAGACGG7350.011.7617085
CGCAAGA8200.011.5787872
TCGCGTA2950.011.2724549
AACCGCG2550.011.17746457
GCGCAAG8450.011.0096871
ACGAACG3100.010.72688715
CAAGACG8250.010.7080344
CCGTCGT6300.010.7075539
CGGTTCT6850.010.54120712
GTCCTAA10200.010.5168051
ACGGTAT6450.010.458549
CGTCGTA6100.010.27984410
GCGTAAC3250.010.23095911
TGTAGGA21450.010.2249592
GTATCAA70150.010.1764371
CGACCAT5800.010.15631410
Produced by FastQC (version 0.11.5)