##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062523_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4267026
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.158817640201864	32.0	32.0	32.0	32.0	32.0
2	30.796533463822342	32.0	32.0	32.0	32.0	32.0
3	30.773807565269113	32.0	32.0	32.0	32.0	32.0
4	30.851598748167927	32.0	32.0	32.0	32.0	32.0
5	30.63093077004921	32.0	32.0	32.0	32.0	32.0
6	34.292359830945486	36.0	36.0	36.0	32.0	36.0
7	34.17696329949712	36.0	36.0	36.0	32.0	36.0
8	34.17393355465845	36.0	36.0	36.0	32.0	36.0
9	34.34466722255735	36.0	36.0	36.0	32.0	36.0
10	33.98401767413651	36.0	36.0	36.0	32.0	36.0
11	34.32534697468448	36.0	36.0	36.0	32.0	36.0
12	34.0877257368481	36.0	36.0	36.0	32.0	36.0
13	34.22096068784207	36.0	36.0	36.0	32.0	36.0
14	34.11938291447017	36.0	36.0	36.0	32.0	36.0
15	34.05597387969982	36.0	36.0	36.0	32.0	36.0
16	34.06417912616422	36.0	36.0	36.0	32.0	36.0
17	33.98310978184806	36.0	36.0	36.0	32.0	36.0
18	33.99538413874207	36.0	36.0	36.0	32.0	36.0
19	33.979137225786765	36.0	36.0	36.0	32.0	36.0
20	33.96994042220507	36.0	36.0	36.0	32.0	36.0
21	33.9572123535221	36.0	36.0	36.0	32.0	36.0
22	33.906807223579136	36.0	36.0	36.0	32.0	36.0
23	33.87438229811583	36.0	36.0	36.0	32.0	36.0
24	33.840359304114855	36.0	36.0	36.0	32.0	36.0
25	33.392378907463886	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	33.0
4	177.0
5	423.0
6	697.0
7	206.0
8	599.0
9	785.0
10	498.0
11	166.0
12	333.0
13	255.0
14	527.0
15	675.0
16	1069.0
17	1612.0
18	2202.0
19	3402.0
20	5500.0
21	8385.0
22	13147.0
23	19722.0
24	27647.0
25	39212.0
26	53407.0
27	68594.0
28	92278.0
29	122260.0
30	156758.0
31	213074.0
32	303266.0
33	435038.0
34	954691.0
35	1740387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.35924621061553	17.47506712516781	11.100941814854536	26.064744849362125
2	16.762333982521145	19.888700616611242	37.40833444656153	25.940630954306084
3	19.5852859113222	23.705286989414773	28.060546616371308	28.648880482891727
4	12.911004562185799	15.278493972401868	35.67067225204717	36.13982921336516
5	14.781629766610536	36.34410346566465	33.410447608984676	15.463819158740144
6	34.13624872862936	35.101810469294605	16.936133935994665	13.825806866081356
7	30.070646891009144	30.289235993447722	20.697322922541694	18.942794193001443
8	27.80004550497623	33.235544546791544	19.583089975535213	19.381319972697014
9	27.437339692688507	14.395696269164743	18.690911786466792	39.47605225167995
10	16.13858256927122	26.614537518838077	31.5283346069983	25.718545304892398
11	37.2707535723646	21.23160438418725	22.36826637928668	19.129375664161476
12	25.01102915639625	23.500433657042986	28.8309422748034	22.65759491175736
13	29.66742508729033	19.732576367450687	25.24351074056407	25.356487804694915
14	23.292406930073923	20.02677325912221	24.99369385329615	31.687125957507718
15	24.93391869970583	27.73408593830285	22.26645369241143	25.06554166957989
16	25.416272702317798	25.864277619931354	24.324543724763796	24.39490595298705
17	23.819957595342434	26.062955617772417	25.460603933177467	24.656482853707686
18	24.564880602345575	24.965493160347805	26.800632832677145	23.66899340462948
19	25.548827019522925	24.95201166175285	25.336739684032576	24.16242163469165
20	25.573234904827856	24.70824693816785	25.258469854705957	24.460048302298336
21	26.327470488271103	24.41425911750861	24.41684035249345	24.841430041726838
22	25.709364967116727	24.9970837967769	24.85738768744967	24.436163548656705
23	24.296688336390844	24.7363644662834	25.618744119757125	25.34820307756863
24	24.686810659607822	25.251541775787782	25.45448358724527	24.60716397735913
25	24.66275332952403	24.999912002712662	25.546058366840747	24.79127630092256
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	361.0
1	361.0
2	1129.0
3	1897.0
4	1897.0
5	1897.0
6	4964.5
7	8032.0
8	8032.0
9	8032.0
10	8867.5
11	9703.0
12	9703.0
13	9703.0
14	11155.5
15	12608.0
16	12608.0
17	12608.0
18	20899.0
19	29190.0
20	29190.0
21	29190.0
22	47846.5
23	66503.0
24	66503.0
25	66503.0
26	100313.0
27	134123.0
28	134123.0
29	134123.0
30	170772.5
31	207422.0
32	207422.0
33	207422.0
34	245397.5
35	283373.0
36	283373.0
37	283373.0
38	317378.0
39	351383.0
40	351383.0
41	351383.0
42	396343.5
43	441304.0
44	441304.0
45	441304.0
46	493168.5
47	545033.0
48	545033.0
49	545033.0
50	547627.0
51	550221.0
52	550221.0
53	550221.0
54	510971.0
55	471721.0
56	471721.0
57	471721.0
58	433581.5
59	395442.0
60	395442.0
61	395442.0
62	351544.5
63	307647.0
64	307647.0
65	307647.0
66	256391.0
67	205135.0
68	205135.0
69	205135.0
70	157912.5
71	110690.0
72	110690.0
73	110690.0
74	87913.0
75	65136.0
76	65136.0
77	65136.0
78	52197.5
79	39259.0
80	39259.0
81	39259.0
82	28254.5
83	17250.0
84	17250.0
85	17250.0
86	12726.0
87	8202.0
88	8202.0
89	8202.0
90	5549.0
91	2896.0
92	2896.0
93	2896.0
94	1775.0
95	654.0
96	654.0
97	654.0
98	1247.5
99	1841.0
100	1841.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00867114472703002
2	0.003655942101126171
3	0.005413606572821445
4	0.015233092088025712
5	0.035036111802459136
6	0.04555866310634151
7	0.0670490407136024
8	0.08802383674249933
9	0.09655436831179375
10	0.11827910118194732
11	0.11888842486546836
12	0.1310983340621782
13	0.11949774854898938
14	0.12446608012231469
15	0.11370917355553961
16	0.11239678408334049
17	0.1225678024928838
18	0.13011404195802884
19	0.1306296235363928
20	0.11991958802219625
21	0.12889539459098678
22	0.1487921564105773
23	0.13908984852681938
24	0.13372311300657647
25	0.12962189590595416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4267026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.83796915089732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.17857002425679	34.743735744538164
2	14.570519547340316	13.649070901417442
3	4.98790638234839	7.008702157919933
4	2.1406783486822887	4.010601058302995
5	1.1120921086864766	2.6044067939806776
6	0.670038992792332	1.8829959404583336
7	0.44029875321062517	1.4435889594040474
8	0.3053449876159155	1.14413912882683
9	0.21430488884979357	0.9033845195549801
>10	1.1821759644448235	10.019108482442372
>50	0.09307413232878238	3.0735417547090615
>100	0.082745834315561	8.064240786667812
>500	0.013812098654378688	4.423006028129645
>1k	0.008337484848855702	6.698790325999128
>5k	1.0045162468500834E-4	0.33068741764874954
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8854	0.20749814976519948	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5203	0.12193504328307352	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4325	0.10135865120109416	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3435526289270324E-5
2	0.0	0.0	0.0	0.0	2.3435526289270324E-5
3	0.0	0.0	0.0	0.0	2.3435526289270324E-5
4	0.0	0.0	0.0	0.0	2.3435526289270324E-5
5	0.0	0.0	0.0	0.0	7.030657886781097E-5
6	0.0	0.0	0.0	0.0	1.1717763144635163E-4
7	0.0	0.0	0.0	0.0	1.1717763144635163E-4
8	0.0	0.0	0.0	4.687105257854065E-5	1.1717763144635163E-4
9	0.0	0.0	0.0	7.030657886781097E-5	1.4061315773562194E-4
10	0.0	0.0	0.0	7.030657886781097E-5	1.4061315773562194E-4
11	0.0	0.0	0.0	1.1717763144635163E-4	1.4061315773562194E-4
12	0.0	0.0	0.0	1.1717763144635163E-4	1.6404868402489228E-4
13	0.0	0.0	0.0	1.1717763144635163E-4	1.6404868402489228E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	265	0.0	13.61267	1
CGAACGA	250	0.0	12.922362	16
CGTTATT	280	0.0	12.885535	2
TATTCCG	85	3.948604E-6	12.293912	5
AAGACGG	735	0.0	11.761708	5
CGCAAGA	820	0.0	11.578787	2
TCGCGTA	295	0.0	11.272454	9
AACCGCG	255	0.0	11.1774645	7
GCGCAAG	845	0.0	11.009687	1
ACGAACG	310	0.0	10.726887	15
CAAGACG	825	0.0	10.708034	4
CCGTCGT	630	0.0	10.707553	9
CGGTTCT	685	0.0	10.541207	12
GTCCTAA	1020	0.0	10.516805	1
ACGGTAT	645	0.0	10.45854	9
CGTCGTA	610	0.0	10.279844	10
GCGTAAC	325	0.0	10.230959	11
TGTAGGA	2145	0.0	10.224959	2
GTATCAA	7015	0.0	10.176437	1
CGACCAT	580	0.0	10.156314	10
>>END_MODULE