FastQCFastQC Report
Thu 2 Feb 2017
SRR4062523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4267026
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA27700.020.7296052
TAGGACG19700.019.5430324
CTGTAGG28900.018.8068451
GTAGGAC29450.018.3023133
GTATCAA85400.017.933631
AGGACGT21300.017.7651545
TAGGACC28200.017.3190464
ATACCGT9950.017.246136
GTCCTAC25500.017.172351
GATATAC12050.017.1655461
TCCTACA27000.016.9484462
ATACGAA11200.016.89275740
TTAGGAC16050.016.8599113
TACCGTC10050.016.8556217
ACCGTCG9800.016.8366378
CGCAATA11200.016.69633136
CGTCGTA9750.016.24605810
GGACGTG23600.016.2202436
TCTAGCG11750.016.10203428
CCGTCGT10400.016.0768289
Produced by FastQC (version 0.11.5)