##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062523_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4267026
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27013404652327	32.0	32.0	32.0	32.0	32.0
2	31.396772365577338	32.0	32.0	32.0	32.0	32.0
3	31.486323964278633	32.0	32.0	32.0	32.0	32.0
4	31.585631069508366	32.0	32.0	32.0	32.0	32.0
5	31.532665842673563	32.0	32.0	32.0	32.0	32.0
6	35.11137171416345	36.0	36.0	36.0	36.0	36.0
7	35.12574167581824	36.0	36.0	36.0	36.0	36.0
8	35.06532231113661	36.0	36.0	36.0	36.0	36.0
9	35.205293335451906	36.0	36.0	36.0	36.0	36.0
10	35.07195737733963	36.0	36.0	36.0	36.0	36.0
11	35.203733466822094	36.0	36.0	36.0	36.0	36.0
12	35.116396056644604	36.0	36.0	36.0	36.0	36.0
13	35.15911433396469	36.0	36.0	36.0	36.0	36.0
14	35.120650776442425	36.0	36.0	36.0	36.0	36.0
15	35.08423735875994	36.0	36.0	36.0	36.0	36.0
16	35.09617986860169	36.0	36.0	36.0	36.0	36.0
17	35.074678710652336	36.0	36.0	36.0	36.0	36.0
18	35.08671074420451	36.0	36.0	36.0	36.0	36.0
19	35.06794498088364	36.0	36.0	36.0	36.0	36.0
20	35.05832586911821	36.0	36.0	36.0	36.0	36.0
21	35.05391905275478	36.0	36.0	36.0	36.0	36.0
22	35.034052054053575	36.0	36.0	36.0	36.0	36.0
23	34.99745466748972	36.0	36.0	36.0	36.0	36.0
24	34.979989107167384	36.0	36.0	36.0	36.0	36.0
25	34.95021052133266	36.0	36.0	36.0	32.0	36.0
26	34.90955996049708	36.0	36.0	36.0	32.0	36.0
27	34.89222868574037	36.0	36.0	36.0	32.0	36.0
28	34.874282697129104	36.0	36.0	36.0	32.0	36.0
29	34.85148906990489	36.0	36.0	36.0	32.0	36.0
30	34.838529926932715	36.0	36.0	36.0	32.0	36.0
31	34.83906378822159	36.0	36.0	36.0	32.0	36.0
32	34.796319731822585	36.0	36.0	36.0	32.0	36.0
33	34.78024155465657	36.0	36.0	36.0	32.0	36.0
34	34.76489222235815	36.0	36.0	36.0	32.0	36.0
35	34.746484085168454	36.0	36.0	36.0	32.0	36.0
36	34.715496694887726	36.0	36.0	36.0	32.0	36.0
37	34.70891295248728	36.0	36.0	36.0	32.0	36.0
38	34.674471399986786	36.0	36.0	36.0	32.0	36.0
39	34.65499928990355	36.0	36.0	36.0	32.0	36.0
40	34.65610849336282	36.0	36.0	36.0	32.0	36.0
41	34.62859799776238	36.0	36.0	36.0	32.0	36.0
42	34.60450088656596	36.0	36.0	36.0	32.0	36.0
43	34.595528595326115	36.0	36.0	36.0	32.0	36.0
44	34.56246880145563	36.0	36.0	36.0	32.0	36.0
45	34.524985786353305	36.0	36.0	36.0	32.0	36.0
46	34.50968613737062	36.0	36.0	36.0	32.0	36.0
47	34.48788570775055	36.0	36.0	36.0	32.0	36.0
48	34.445568177930014	36.0	36.0	36.0	32.0	36.0
49	34.431843396313965	36.0	36.0	36.0	32.0	36.0
50	33.91089859775872	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	27.0
21	107.0
22	350.0
23	1053.0
24	2747.0
25	6467.0
26	13486.0
27	25037.0
28	42533.0
29	64976.0
30	94861.0
31	137831.0
32	203862.0
33	333898.0
34	733111.0
35	2606675.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83712584843472	18.069368313134348	11.64716460254651	26.446341235884425
2	16.13565116797942	20.41838299675452	37.09677570557807	26.349190129687994
3	18.999592688262158	23.989372397763116	28.433546550950762	28.577488363023967
4	12.622635438293308	15.79686699162436	35.85973526704956	35.72076230303278
5	14.518964730939066	36.576177412558536	33.34071552411446	15.564142332387945
6	34.36242312234313	35.290356917623996	16.652187352868182	13.695032607164695
7	30.088035575137244	30.356138058717725	20.679981954640528	18.875844411504502
8	27.81567302378753	33.419786989814455	19.435550662217665	19.328989324180355
9	27.468803799180037	14.230543708897017	18.607081372365673	39.69357111955728
10	16.10008938309727	26.710102071091203	31.511877359078667	25.677931186732867
11	37.52744886016631	21.052437927493294	22.339821693141783	19.080291519198617
12	25.035282184828496	23.39432663405379	28.776693650331637	22.793697530786076
13	29.847439410962107	19.580710312053405	25.253068530634685	25.3187817463498
14	23.36784448934691	19.901589538006096	24.926775698109175	31.803790274537818
15	25.0234706795787	27.70613537391148	22.142002415734048	25.12839153077577
16	25.514093422444578	25.812615156317303	24.257363325182457	24.415928096055662
17	23.966833105774374	26.01563712056125	25.28695630164897	24.730573472015404
18	24.741142894371865	24.902285573136886	26.725452340810673	23.631119191680575
19	25.728059777465617	24.86270296923431	25.25480275958009	24.154434493719982
20	25.70903013011873	24.623168455031678	25.196096766225466	24.471704648624122
21	26.518024497624342	24.37472844083912	24.336692581671638	24.7705544798649
22	25.806076644482594	24.944750746773046	24.773882324597977	24.475290284146382
23	24.383282407934708	24.664391545774507	25.595461569720925	25.35686447656986
24	24.839103394261013	25.099987672913173	25.415523598871907	24.645385333953907
25	24.83197899426908	24.818714486389347	25.470573650125406	24.878732869216172
26	24.2059457805038	25.532185648739897	25.95946216404587	24.302406406710436
27	25.06790443742316	25.149905343909317	25.107041766326244	24.67514845234128
28	24.226967447585274	25.178356072824492	25.555011851345643	25.03966462824459
29	24.41112381316636	25.003503611180243	25.347279346317553	25.23809322933584
30	24.291063611986427	25.24936571748098	25.685219635408828	24.77435103512376
31	24.99016879672165	24.896496998143437	24.843603015308556	25.269731189826356
32	24.552182245901477	25.27099670824598	24.9754278506857	25.201393195166844
33	24.19303280551841	24.87849851395328	25.736801228771515	25.1916674517568
34	24.767437554868426	24.970436083586087	25.918403121987073	24.343723239558418
35	25.17908257413946	25.01590100458727	25.581611173683967	24.223405247589305
36	24.129874062168827	25.31554764372188	25.514913665864704	25.03966462824459
37	25.08916045976753	25.044281426923575	24.927455328371565	24.93910278493733
38	24.425396048676525	25.019158542741476	25.478166760643127	25.077278647938865
39	25.067506033476246	24.4855081736085	25.213134393837773	25.233851399077484
40	25.484822450109277	24.60988051162566	25.633755219677596	24.271541818587465
41	24.30505462118112	24.75961008908781	26.379708021465063	24.555627268266
42	25.162537092579235	25.30661870820567	25.62332641047887	23.90751778873623
43	24.17046439370184	24.525840714352338	26.046126740263592	25.257568151682225
44	23.819283032257125	24.97060013227011	25.79763985501846	25.4124769804543
45	23.92612559660991	25.164107272840614	25.84125336944279	25.06851376110668
46	23.931871907825155	24.514268854078757	26.02166710686448	25.5321921312316
47	24.29111048303901	24.500108506486722	26.226392808480657	24.982388201993615
48	24.474587218357705	25.265489359568	25.36975401602896	24.890169406045334
49	23.887738204548086	25.050679325600544	25.840198770759777	25.221383699091593
50	23.554115572324978	25.725956609112906	25.422466472542343	25.297461346019766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	27.0
2	37.0
3	135.0
4	233.0
5	284.0
6	335.0
7	433.0
8	531.0
9	710.5
10	890.0
11	1212.0
12	1534.0
13	2767.0
14	4000.0
15	5649.0
16	7298.0
17	8878.5
18	10459.0
19	11878.5
20	13298.0
21	15528.5
22	17759.0
23	19762.5
24	21766.0
25	25808.5
26	29851.0
27	39317.5
28	48784.0
29	57426.5
30	66069.0
31	73752.5
32	81436.0
33	95695.0
34	109954.0
35	128219.0
36	146484.0
37	164677.0
38	182870.0
39	190199.0
40	197528.0
41	208097.5
42	218667.0
43	222487.0
44	226307.0
45	245535.5
46	264764.0
47	281360.0
48	297956.0
49	306528.5
50	315101.0
51	304167.0
52	293233.0
53	287325.0
54	281417.0
55	284200.5
56	286984.0
57	279000.0
58	271016.0
59	248184.5
60	225353.0
61	199467.0
62	173581.0
63	152703.0
64	131825.0
65	113101.5
66	94378.0
67	83703.0
68	73028.0
69	66088.0
70	59148.0
71	47509.5
72	35871.0
73	32102.0
74	28333.0
75	21578.0
76	14823.0
77	13176.0
78	11529.0
79	10143.0
80	8757.0
81	7088.0
82	5419.0
83	4677.0
84	3935.0
85	3236.5
86	2538.0
87	1914.0
88	1290.0
89	867.5
90	445.0
91	277.0
92	109.0
93	78.5
94	48.0
95	36.0
96	24.0
97	17.0
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01499873682513301
2	0.003984039469175955
3	5.624526309424878E-4
4	2.1091973660343293E-4
5	0.0
6	4.4527499949613614E-4
7	2.3435526289270324E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1717763144635163E-4
47	0.0
48	0.0
49	0.0
50	2.3435526289270324E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4267026.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.0141991805322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22639634109001	38.37614366578025
2	14.42438016444269	14.716964055291948
3	4.721210897102064	7.225463792341914
4	1.9393921106256418	3.9574614168243705
5	1.0022451687266376	2.55643673325734
6	0.6173439176585787	1.8895983346994887
7	0.39353173569293676	1.4052994443950024
8	0.27597087934093567	1.126274672538009
9	0.19415667952904647	0.8914272769552983
>10	1.0456026178850006	9.500157983017889
>50	0.07660382236365765	2.729365760878275
>100	0.06696071681004014	7.146541610281931
>500	0.010572459560668802	3.756765525794842
>1k	0.005632489172270152	4.7220997279435695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.37421051570813E-5
2	0.0	0.0	0.0	0.0	9.37421051570813E-5
3	0.0	0.0	0.0	0.0	9.37421051570813E-5
4	0.0	0.0	0.0	0.0	1.4061315773562194E-4
5	2.3435526289270324E-5	0.0	0.0	0.0	1.4061315773562194E-4
6	2.3435526289270324E-5	0.0	0.0	0.0	1.6404868402489228E-4
7	2.3435526289270324E-5	0.0	0.0	0.0	1.6404868402489228E-4
8	2.3435526289270324E-5	0.0	0.0	0.0	1.6404868402489228E-4
9	2.3435526289270324E-5	0.0	0.0	0.0	1.6404868402489228E-4
10	2.3435526289270324E-5	0.0	0.0	0.0	1.6404868402489228E-4
11	2.3435526289270324E-5	0.0	0.0	4.687105257854065E-5	1.6404868402489228E-4
12	2.3435526289270324E-5	0.0	0.0	4.687105257854065E-5	1.6404868402489228E-4
13	4.687105257854065E-5	0.0	0.0	4.687105257854065E-5	1.6404868402489228E-4
14	4.687105257854065E-5	0.0	0.0	4.687105257854065E-5	1.6404868402489228E-4
15	4.687105257854065E-5	0.0	0.0	7.030657886781097E-5	2.3435526289270327E-4
16	4.687105257854065E-5	0.0	0.0	1.1717763144635163E-4	2.577907891819736E-4
17	4.687105257854065E-5	0.0	0.0	1.1717763144635163E-4	2.577907891819736E-4
18	4.687105257854065E-5	0.0	0.0	2.1091973660343293E-4	3.515328943390549E-4
19	4.687105257854065E-5	0.0	0.0	3.046618417605142E-4	3.515328943390549E-4
20	4.687105257854065E-5	0.0	0.0	4.2183947320686586E-4	3.515328943390549E-4
21	4.687105257854065E-5	0.0	0.0	5.624526309424878E-4	3.515328943390549E-4
22	4.687105257854065E-5	0.0	0.0	9.139855252815427E-4	3.515328943390549E-4
23	4.687105257854065E-5	0.0	0.0	0.0016170513139596525	3.984039469175955E-4
24	4.687105257854065E-5	0.0	0.0	0.0024841657866626547	4.2183947320686586E-4
25	4.687105257854065E-5	0.0	0.0	0.003093489470183683	4.2183947320686586E-4
26	4.687105257854065E-5	0.0	0.0	0.004124652626911578	4.6871052578540654E-4
27	4.687105257854065E-5	0.0	0.0	0.006351027624392258	4.6871052578540654E-4
28	4.687105257854065E-5	0.0	0.0	0.01633456182362142	4.6871052578540654E-4
29	4.687105257854065E-5	0.0	0.0	0.03848113416698187	5.624526309424878E-4
30	4.687105257854065E-5	0.0	0.0	0.07513429728340067	5.624526309424878E-4
31	4.687105257854065E-5	0.0	0.0	0.16374402218313178	6.093236835210285E-4
32	4.687105257854065E-5	0.0	0.0	0.2618920062825959	6.561947360995691E-4
33	4.687105257854065E-5	0.0	0.0	0.35999311932948147	6.561947360995691E-4
34	4.687105257854065E-5	0.0	0.0	0.468405863943646	6.561947360995691E-4
35	7.030657886781097E-5	0.0	0.0	0.5890050822282311	6.561947360995691E-4
36	7.030657886781097E-5	0.0	0.0	0.7553738833557612	6.561947360995691E-4
37	7.030657886781097E-5	0.0	0.0	0.9997829870265613	6.561947360995691E-4
38	7.030657886781097E-5	0.0	0.0	1.3144283629863047	6.796302623888395E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGGA	2770	0.0	20.729605	2
TAGGACG	1970	0.0	19.543032	4
CTGTAGG	2890	0.0	18.806845	1
GTAGGAC	2945	0.0	18.302313	3
GTATCAA	8540	0.0	17.93363	1
AGGACGT	2130	0.0	17.765154	5
TAGGACC	2820	0.0	17.319046	4
ATACCGT	995	0.0	17.24613	6
GTCCTAC	2550	0.0	17.17235	1
GATATAC	1205	0.0	17.165546	1
TCCTACA	2700	0.0	16.948446	2
ATACGAA	1120	0.0	16.892757	40
TTAGGAC	1605	0.0	16.859911	3
TACCGTC	1005	0.0	16.855621	7
ACCGTCG	980	0.0	16.836637	8
CGCAATA	1120	0.0	16.696331	36
CGTCGTA	975	0.0	16.246058	10
GGACGTG	2360	0.0	16.220243	6
TCTAGCG	1175	0.0	16.102034	28
CCGTCGT	1040	0.0	16.076828	9
>>END_MODULE