FastQCFastQC Report
Thu 2 Feb 2017
SRR4062522_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062522_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384635
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60771.5799394230894226No Hit
GTACATGGAAGCAGTGGTATCAACG55411.4405865300869656No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49721.2926540746421933No Hit
TCCATGTACTCTGCGTTGATACCAC46581.2110182380698584No Hit
TATCAACGCAGAGTACTTTTTTTTT39201.0191480234508041No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37410.9726103968697596No Hit
GAGTACATGGAAGCAGTGGTATCAA25900.673365658351424No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25400.6603663213176127No Hit
CATGTACTCTGCGTTGATACCACTG22150.5758706305978395No Hit
GGTATCAACGCAGAGTACTTTTTTT20890.5431123012726351No Hit
GTACATGGGGTGGTATCAACGCAAA18270.474995775215464No Hit
GTCCTACAGTGGACATTTCTAAATT17450.45367686248001354No Hit
GTCCTAAAGTGTGTATTTCTCATTT17180.4466572204817555No Hit
CTGTAGGACGTGGAATATGGCAAGA15320.39829968671597743No Hit
CTTTAGGACGTGAAATATGGCGAGG14370.3736009463517361No Hit
GCTTCCATGTACTCTGCGTTGATAC14130.3673612645755066No Hit
CATGGAAGCAGTGGTATCAACGCAG12730.33096312088083507No Hit
GCGTTGATACCACTGCTTCCATGTA12550.326283359548663No Hit
GTCCTACAGTGTGCATTTCTCATTT12180.3166638501436427No Hit
GCAGAGTACTTTTTTTTTTTTTTTT12060.31354400925552794No Hit
CCCATGTACTCTGCGTTGATACCAC11660.3031445396284789No Hit
GTACATGGGTGGTATCAACGCAAAA11370.2956049241488684No Hit
GTATCAACGCAGAGTACATGGAAGC10850.28208561363370466No Hit
GTATCAACGCAGAGTACATGGGAAG10250.26648640919313116No Hit
GTACATGGGAAGCAGTGGTATCAAC9260.24074772186618482No Hit
GTACATGGGAGTGGTATCAACGCAA9200.23918780142212748No Hit
ACGCAGAGTACATGGAAGCAGTGGT8720.22670843786966863No Hit
CTGTAGGACCTGGAATATGGCGAGA8670.22540850416628752No Hit
GTATCAACGCAGAGTACATGGGGTG8340.2168289417239721No Hit
ACTCTGCGTTGATACCACTGCTTCC8220.21370910083585737No Hit
CAGTGGTATCAACGCAGAGTACATG7810.20304964446813215No Hit
ATCAACGCAGAGTACTTTTTTTTTT7590.19732993617325514No Hit
CTGAAGGACCTGGAATATGGCGAGA6990.18173073173268162No Hit
GTACATGGGCAGTGGTATCAACGCA6510.1692513681802228No Hit
TATCAACGCAGAGTACATGGAAGCA6470.16821142121751792No Hit
ATTTAGAAATGTCCACTGTAGGACG6460.16795143447684166No Hit
GTCCTTCAGTGTGCATTTCTCATTT6030.15677200462776397No Hit
TTTCTAAATTTTCCACCTTTTTCAG5930.15417213722100173No Hit
GGTATCAACGCAGAGTACATGGGAA5720.148712415666801No Hit
TATCAACGCAGAGTACATGGGAAGC5490.1427327206312478No Hit
GTATCAACGCAGAGTACATGGGCAG5460.14195276040921911No Hit
TTGTAGAACAGTGTATATCAATGAG5370.1396128797431331No Hit
TATCAACGCAGAGTACATGGGGTGG5370.1396128797431331No Hit
GTATTAGAGGCACTGCCTGCCCAGT5330.1385729327804282No Hit
GAATATGGCAAGAAAACTGAAAATC5310.13805295929907577No Hit
GATATACACTGTTCTACAAATCCCG5240.13623305211434217No Hit
ATACCACTGCTTCCATGTACTCTGC5130.1333731979669037No Hit
GCGTTGATACCACTGCTTCCCATGT4780.1242736620432358No Hit
GGTATCAACGCAGAGTACATGGAAG4670.12141380789579732No Hit
CCATAGGGTCTTCTCGTCTTATTAT4630.12037386093309241No Hit
AAGCAGTGGTATCAACGCAGAGTAC4620.12011387419241619No Hit
CTGTAGGACATGGAATATGGCAAGA4550.1182939670076826No Hit
GTGTATATCAATGAGTTACAATGAA4490.11673404656362527No Hit
GGTATCAACGCAGAGTACATGGGGT4440.11543411286024413No Hit
GTATCAACGCAGAGTACATGGGAGT4330.11257425871280566No Hit
GGAATATGGCGAGAAAACTGAAAAT4200.1091944310840147No Hit
ACGCAGAGTACATGGGGTGGTATCA4190.1089344443433385No Hit
CTCTAATACTTGTAATGCTAGAGGT4070.10581460345522378No Hit
GTATCAACGCAGAGTACATGGGTGG4050.10529462997387133No Hit
CCACTGCTTCCATGTACTCTGCGTT4040.1050346432331951No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3870.10061486864169927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAGT402.756063E-416.62363411
CGAGTTC350.002167392816.28437613
AGCTTGA350.002167392816.28437612
AACCAGC350.002167392816.28437611
ACTTATC350.002167392816.2843768
GTTTAGA350.002169278216.2822571
CCAACGA755.7749276E-815.20666619
AGCATTA508.692857E-515.198756
AGATAGA704.4148874E-714.92734410
CGTCTTA456.750159E-414.77656415
TTGAACG456.750159E-414.77656415
AACGGAC653.3590495E-614.61418315
TAGATAG653.3590495E-614.6141839
AACGCTT400.00525422414.2562518
ACGCTTT400.00525422414.2562519
GAACGCT400.00525422414.2562517
GTTTGCC400.00527241214.2488297
TGGGTAA400.00527241214.2488296
GACTTTA400.00527241214.2488297
AGATTAT400.00527241214.2488295
Produced by FastQC (version 0.11.5)