##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062522_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384635
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.386275299959703	32.0	32.0	32.0	32.0	32.0
2	30.44861492063905	32.0	32.0	32.0	32.0	32.0
3	30.506383974417304	32.0	32.0	32.0	32.0	32.0
4	30.340325243412586	32.0	32.0	32.0	21.0	32.0
5	30.433418695646523	32.0	32.0	32.0	32.0	32.0
6	33.82497172644195	36.0	36.0	36.0	32.0	36.0
7	33.46698818360264	36.0	36.0	36.0	21.0	36.0
8	33.41170200319784	36.0	36.0	36.0	21.0	36.0
9	33.41981878924175	36.0	36.0	36.0	21.0	36.0
10	33.370483705331026	36.0	36.0	36.0	21.0	36.0
11	33.77270659196381	36.0	36.0	36.0	32.0	36.0
12	33.59927463699351	36.0	36.0	36.0	27.0	36.0
13	33.68701496223692	36.0	36.0	36.0	27.0	36.0
14	33.63317170824288	36.0	36.0	36.0	27.0	36.0
15	33.68116786043912	36.0	36.0	36.0	27.0	36.0
16	33.633371898033204	36.0	36.0	36.0	27.0	36.0
17	33.571175270061225	36.0	36.0	36.0	27.0	36.0
18	33.68638579432449	36.0	36.0	36.0	27.0	36.0
19	33.56977654139639	36.0	36.0	36.0	27.0	36.0
20	33.43610955841252	36.0	36.0	36.0	21.0	36.0
21	33.310717953384376	36.0	36.0	36.0	21.0	36.0
22	33.327076319107725	36.0	36.0	36.0	21.0	36.0
23	33.39025309709205	36.0	36.0	36.0	21.0	36.0
24	33.417861089084454	36.0	36.0	36.0	21.0	36.0
25	32.98139534883721	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	12.0
5	27.0
6	66.0
7	26.0
8	61.0
9	69.0
10	40.0
11	17.0
12	22.0
13	20.0
14	224.0
15	244.0
16	311.0
17	461.0
18	666.0
19	1024.0
20	1454.0
21	2163.0
22	3021.0
23	4001.0
24	4697.0
25	5461.0
26	6573.0
27	7748.0
28	9699.0
29	11840.0
30	14883.0
31	19287.0
32	26649.0
33	36615.0
34	78280.0
35	148972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.45599274064175	19.165277803056107	12.6796964173819	21.69903303892025
2	11.784954735249308	22.659414600157035	44.222439460670884	21.333191203922773
3	19.80105349092365	30.73843161928731	28.677988487517748	20.782526402271298
4	10.486053870775663	17.734608814130485	37.95266090637489	33.82667640871897
5	9.918157064577118	42.12089452481945	34.61173368147572	13.349214729127713
6	27.63071993174967	37.62975756046683	20.74638395492981	13.99313855285369
7	24.84064999193494	34.432089579420044	22.928189731875726	17.79907069676929
8	26.32578714545928	35.68019776216498	21.667707520166537	16.326307572209213
9	28.353839427911186	15.182316876411745	21.55351882501119	34.910324870665875
10	16.23270644888397	28.700201730981973	33.82104509663565	21.246046723498406
11	31.949982820910595	23.246691723843536	26.084104657094965	18.719220798150904
12	25.264876009392363	26.09738065611154	32.24901208407307	16.38873125042302
13	30.825353278481572	23.243628753705877	27.655282453596815	18.275735514215732
14	22.112230979873598	23.769821850629405	28.66398384057141	25.453963328925582
15	21.97068285217536	32.25588997740831	29.14771011837215	16.625717052044184
16	19.432399652277116	28.221251698272315	33.13101552780482	19.21533312164575
17	17.569168057099276	29.417996579665935	32.54315113243253	20.46968423080227
18	18.811028069983628	25.53163242196347	38.58080370274921	17.0765358053037
19	21.297648519684177	28.235725944379798	31.11721997912225	19.349405556813775
20	21.865799660034828	26.16091858276694	35.28765582821607	16.685625928982162
21	21.639456065638633	26.378838204516818	28.800460220114328	23.181245509730218
22	20.9846360533325	30.760698915072602	29.93144675211739	18.32321827947751
23	18.41541421404639	30.292908597209678	31.90024810407354	19.3914290846704
24	20.30894905776785	29.74819141929656	32.31495726829471	17.627902254640873
25	20.793537991373228	29.162078650954175	31.960442844387522	18.08394051328507
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	338.0
1	338.0
2	792.5
3	1247.0
4	1247.0
5	1247.0
6	3713.5
7	6180.0
8	6180.0
9	6180.0
10	5818.5
11	5457.0
12	5457.0
13	5457.0
14	3935.5
15	2414.0
16	2414.0
17	2414.0
18	4052.0
19	5690.0
20	5690.0
21	5690.0
22	9255.0
23	12820.0
24	12820.0
25	12820.0
26	19736.5
27	26653.0
28	26653.0
29	26653.0
30	33826.5
31	41000.0
32	41000.0
33	41000.0
34	40681.5
35	40363.0
36	40363.0
37	40363.0
38	38575.0
39	36787.0
40	36787.0
41	36787.0
42	39147.5
43	41508.0
44	41508.0
45	41508.0
46	55064.0
47	68620.0
48	68620.0
49	68620.0
50	55557.0
51	42494.0
52	42494.0
53	42494.0
54	30497.0
55	18500.0
56	18500.0
57	18500.0
58	14997.5
59	11495.0
60	11495.0
61	11495.0
62	9929.5
63	8364.0
64	8364.0
65	8364.0
66	7372.5
67	6381.0
68	6381.0
69	6381.0
70	5191.0
71	4001.0
72	4001.0
73	4001.0
74	3195.0
75	2389.0
76	2389.0
77	2389.0
78	1740.0
79	1091.0
80	1091.0
81	1091.0
82	752.5
83	414.0
84	414.0
85	414.0
86	291.5
87	169.0
88	169.0
89	169.0
90	100.0
91	31.0
92	31.0
93	31.0
94	23.0
95	15.0
96	15.0
97	15.0
98	114.5
99	214.0
100	214.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007279628738934315
2	0.0023398806660860296
3	0.0023398806660860296
4	0.012479363552458825
5	0.030678435399794608
6	0.043677772433605884
7	0.06785653931649487
8	0.08709555812653555
9	0.09333523990276496
10	0.11959390071106374
11	0.1162140730822728
12	0.12817346315337916
13	0.11647405982294903
14	0.12037386093309241
15	0.10997439130604339
16	0.10945441782469094
17	0.12011387419241619
18	0.12687352944999805
19	0.12843344989405542
20	0.1242736620432358
21	0.12245375485850221
22	0.14403265433462895
23	0.13571307863298973
24	0.12843344989405542
25	0.12531360900594068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	384635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.90463926098984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.08523672401518	25.85918462416786
2	13.88492023371537	9.692962638509075
3	4.832997873578931	5.060821419792106
4	2.26807683957642	3.1666561560648345
5	1.166081323487128	2.03508239726479
6	0.7062010392134983	1.4789815511689979
7	0.4485850063671656	1.096038847759432
8	0.29426527812129455	0.8216978703886905
9	0.2271995666357774	0.713728702230753
>10	1.6141767018360682	11.426261541659137
>50	0.23836437272086167	5.887975882272333
>100	0.19888527348896895	13.82163728259283
>500	0.01713243928931193	4.020130843932474
>1k	0.01638755062455924	11.898152094168795
>5k	0.0014897773295053853	3.0206881480279066
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6077	1.5799394230894226	No Hit
GTACATGGAAGCAGTGGTATCAACG	5541	1.4405865300869656	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4972	1.2926540746421933	No Hit
TCCATGTACTCTGCGTTGATACCAC	4658	1.2110182380698584	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3920	1.0191480234508041	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3741	0.9726103968697596	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2590	0.673365658351424	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2540	0.6603663213176127	No Hit
CATGTACTCTGCGTTGATACCACTG	2215	0.5758706305978395	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2089	0.5431123012726351	No Hit
GTACATGGGGTGGTATCAACGCAAA	1827	0.474995775215464	No Hit
GTCCTACAGTGGACATTTCTAAATT	1745	0.45367686248001354	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1718	0.4466572204817555	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1532	0.39829968671597743	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1437	0.3736009463517361	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1413	0.3673612645755066	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1273	0.33096312088083507	No Hit
GCGTTGATACCACTGCTTCCATGTA	1255	0.326283359548663	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1218	0.3166638501436427	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1206	0.31354400925552794	No Hit
CCCATGTACTCTGCGTTGATACCAC	1166	0.3031445396284789	No Hit
GTACATGGGTGGTATCAACGCAAAA	1137	0.2956049241488684	No Hit
GTATCAACGCAGAGTACATGGAAGC	1085	0.28208561363370466	No Hit
GTATCAACGCAGAGTACATGGGAAG	1025	0.26648640919313116	No Hit
GTACATGGGAAGCAGTGGTATCAAC	926	0.24074772186618482	No Hit
GTACATGGGAGTGGTATCAACGCAA	920	0.23918780142212748	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	872	0.22670843786966863	No Hit
CTGTAGGACCTGGAATATGGCGAGA	867	0.22540850416628752	No Hit
GTATCAACGCAGAGTACATGGGGTG	834	0.2168289417239721	No Hit
ACTCTGCGTTGATACCACTGCTTCC	822	0.21370910083585737	No Hit
CAGTGGTATCAACGCAGAGTACATG	781	0.20304964446813215	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	759	0.19732993617325514	No Hit
CTGAAGGACCTGGAATATGGCGAGA	699	0.18173073173268162	No Hit
GTACATGGGCAGTGGTATCAACGCA	651	0.1692513681802228	No Hit
TATCAACGCAGAGTACATGGAAGCA	647	0.16821142121751792	No Hit
ATTTAGAAATGTCCACTGTAGGACG	646	0.16795143447684166	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	603	0.15677200462776397	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	593	0.15417213722100173	No Hit
GGTATCAACGCAGAGTACATGGGAA	572	0.148712415666801	No Hit
TATCAACGCAGAGTACATGGGAAGC	549	0.1427327206312478	No Hit
GTATCAACGCAGAGTACATGGGCAG	546	0.14195276040921911	No Hit
TTGTAGAACAGTGTATATCAATGAG	537	0.1396128797431331	No Hit
TATCAACGCAGAGTACATGGGGTGG	537	0.1396128797431331	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	533	0.1385729327804282	No Hit
GAATATGGCAAGAAAACTGAAAATC	531	0.13805295929907577	No Hit
GATATACACTGTTCTACAAATCCCG	524	0.13623305211434217	No Hit
ATACCACTGCTTCCATGTACTCTGC	513	0.1333731979669037	No Hit
GCGTTGATACCACTGCTTCCCATGT	478	0.1242736620432358	No Hit
GGTATCAACGCAGAGTACATGGAAG	467	0.12141380789579732	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	463	0.12037386093309241	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	462	0.12011387419241619	No Hit
CTGTAGGACATGGAATATGGCAAGA	455	0.1182939670076826	No Hit
GTGTATATCAATGAGTTACAATGAA	449	0.11673404656362527	No Hit
GGTATCAACGCAGAGTACATGGGGT	444	0.11543411286024413	No Hit
GTATCAACGCAGAGTACATGGGAGT	433	0.11257425871280566	No Hit
GGAATATGGCGAGAAAACTGAAAAT	420	0.1091944310840147	No Hit
ACGCAGAGTACATGGGGTGGTATCA	419	0.1089344443433385	No Hit
CTCTAATACTTGTAATGCTAGAGGT	407	0.10581460345522378	No Hit
GTATCAACGCAGAGTACATGGGTGG	405	0.10529462997387133	No Hit
CCACTGCTTCCATGTACTCTGCGTT	404	0.1050346432331951	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	387	0.10061486864169927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.599867406762255E-4	0.0
10	0.0	0.0	0.0	2.599867406762255E-4	0.0
11	0.0	0.0	0.0	2.599867406762255E-4	0.0
12	0.0	0.0	0.0	5.19973481352451E-4	0.0
13	0.0	0.0	0.0	5.19973481352451E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGAGT	40	2.756063E-4	16.623634	11
CGAGTTC	35	0.0021673928	16.284376	13
AGCTTGA	35	0.0021673928	16.284376	12
AACCAGC	35	0.0021673928	16.284376	11
ACTTATC	35	0.0021673928	16.284376	8
GTTTAGA	35	0.0021692782	16.282257	1
CCAACGA	75	5.7749276E-8	15.206666	19
AGCATTA	50	8.692857E-5	15.19875	6
AGATAGA	70	4.4148874E-7	14.927344	10
CGTCTTA	45	6.750159E-4	14.776564	15
TTGAACG	45	6.750159E-4	14.776564	15
AACGGAC	65	3.3590495E-6	14.614183	15
TAGATAG	65	3.3590495E-6	14.614183	9
AACGCTT	40	0.005254224	14.25625	18
ACGCTTT	40	0.005254224	14.25625	19
GAACGCT	40	0.005254224	14.25625	17
GTTTGCC	40	0.005272412	14.248829	7
TGGGTAA	40	0.005272412	14.248829	6
GACTTTA	40	0.005272412	14.248829	7
AGATTAT	40	0.005272412	14.248829	5
>>END_MODULE