##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384635
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.7040752921601	32.0	32.0	32.0	32.0	32.0
2	31.384777776333408	32.0	32.0	32.0	32.0	32.0
3	31.504514669751842	32.0	32.0	32.0	32.0	32.0
4	31.632032966318718	32.0	32.0	32.0	32.0	32.0
5	31.563562858294226	32.0	32.0	32.0	32.0	32.0
6	35.12719331314103	36.0	36.0	36.0	36.0	36.0
7	35.139649277886825	36.0	36.0	36.0	36.0	36.0
8	35.10730172761189	36.0	36.0	36.0	36.0	36.0
9	35.12631715782495	36.0	36.0	36.0	36.0	36.0
10	35.08475567746045	36.0	36.0	36.0	36.0	36.0
11	35.16198993851314	36.0	36.0	36.0	36.0	36.0
12	35.08211161230777	36.0	36.0	36.0	36.0	36.0
13	35.07553914750348	36.0	36.0	36.0	36.0	36.0
14	35.03323670492805	36.0	36.0	36.0	36.0	36.0
15	35.05321668594902	36.0	36.0	36.0	36.0	36.0
16	35.04976146216543	36.0	36.0	36.0	36.0	36.0
17	35.06851430577041	36.0	36.0	36.0	36.0	36.0
18	35.04018615050632	36.0	36.0	36.0	36.0	36.0
19	34.958383922419955	36.0	36.0	36.0	36.0	36.0
20	34.862248625320106	36.0	36.0	36.0	32.0	36.0
21	34.7851989548533	36.0	36.0	36.0	32.0	36.0
22	34.8054597215542	36.0	36.0	36.0	32.0	36.0
23	34.791155251082195	36.0	36.0	36.0	32.0	36.0
24	34.804479571541854	36.0	36.0	36.0	32.0	36.0
25	34.77087628530945	36.0	36.0	36.0	32.0	36.0
26	34.72449725064022	36.0	36.0	36.0	32.0	36.0
27	34.69828278757783	36.0	36.0	36.0	32.0	36.0
28	34.62239785770926	36.0	36.0	36.0	32.0	36.0
29	34.579734033564286	36.0	36.0	36.0	32.0	36.0
30	34.516065880640085	36.0	36.0	36.0	32.0	36.0
31	34.47090098404981	36.0	36.0	36.0	32.0	36.0
32	34.386431292004104	36.0	36.0	36.0	32.0	36.0
33	34.280101914802344	36.0	36.0	36.0	32.0	36.0
34	34.163328870227616	36.0	36.0	36.0	32.0	36.0
35	34.03049644468132	36.0	36.0	36.0	32.0	36.0
36	33.94857202282684	36.0	36.0	36.0	32.0	36.0
37	33.83353308981242	36.0	36.0	36.0	32.0	36.0
38	33.7439468587102	36.0	36.0	36.0	27.0	36.0
39	33.48481807427821	36.0	36.0	36.0	21.0	36.0
40	33.40183030665436	36.0	36.0	36.0	21.0	36.0
41	33.345018004081794	36.0	36.0	36.0	14.0	36.0
42	33.13642024256763	36.0	36.0	36.0	14.0	36.0
43	33.124026674639595	36.0	36.0	36.0	14.0	36.0
44	33.00085795624423	36.0	36.0	36.0	14.0	36.0
45	32.72679033369298	36.0	36.0	36.0	14.0	36.0
46	32.640643727169916	36.0	36.0	36.0	14.0	36.0
47	32.437399092646274	36.0	36.0	36.0	14.0	36.0
48	32.325654191636225	36.0	36.0	36.0	14.0	36.0
49	32.23433644884111	36.0	36.0	36.0	14.0	36.0
50	31.685046862610008	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	21.0
22	74.0
23	187.0
24	520.0
25	1286.0
26	2807.0
27	5577.0
28	9017.0
29	12356.0
30	15778.0
31	19994.0
32	27552.0
33	35177.0
34	59436.0
35	194846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.70542043049336	20.09740445470667	13.127499466953733	22.069675647846232
2	11.645381367389811	23.002943195290886	43.573590282255545	21.77808515506375
3	19.598942368439527	30.507262767365255	29.20056261423227	20.69323224996295
4	10.478245609473918	17.990302494572777	37.9627439000611	33.56870799589221
5	9.856357325776385	41.9158422920431	34.633873672442704	13.593926709737802
6	28.09468717095428	37.64270022228866	20.07357624761137	14.189036359145685
7	24.843033005316727	34.308370273116076	22.85439442588428	17.99420229568292
8	26.48432929920574	35.6979994020305	21.463985336747825	16.353685962015938
9	28.10586660080336	14.783886021812886	21.507663109181433	35.60258426820232
10	16.351086094609173	28.52652514721749	33.754598515475706	21.367790242697623
11	31.994488281097667	23.064203725609993	26.08836949315585	18.852938500136492
12	25.224433553888755	26.310398169693343	32.16113978187112	16.30402849454678
13	31.050736412442966	22.93135050112444	27.889037659079385	18.128875427353204
14	22.170889284646485	23.67465259271777	28.777152365229377	25.377305757406372
15	22.322461554460723	32.17907886697778	28.785731927671687	16.712727650889807
16	19.51382479493546	28.006811652605716	32.93954008345574	19.539823469003082
17	17.58706305978395	29.22172969178572	32.44582526291159	20.745381985518737
18	19.08458668607901	24.879171162270726	38.77650239837768	17.259739753272584
19	21.676134517139626	27.7171864234924	31.453455873750443	19.15322318561753
20	22.26604443173398	25.75090670375811	35.279940723023124	16.703108141484783
21	21.64415614803645	26.012193378137717	29.29244608524965	23.05120438857618
22	21.11378319705695	30.089565432162964	30.22397857709257	18.572672793687524
23	18.622590247897357	29.546453130890328	32.07300427678189	19.757952344430436
24	20.505674210615258	29.283346549325984	32.43932559439469	17.77165364566407
25	20.94505180235808	28.617260519713493	32.00384780376201	18.43383987416642
26	15.864650902803955	29.445058302026595	34.83666333017016	19.853627464999285
27	19.167522456354728	30.811028637539483	33.17638800421179	16.845060901894005
28	17.724856032342352	28.88634679631339	32.28073368258219	21.108063488762074
29	18.184512589857917	28.88686676979474	32.84880471095974	20.0798159293876
30	19.251498173593145	27.929855577365554	33.48993201346731	19.328714235573983
31	18.80406099288936	27.947014702250183	32.520181470744994	20.72874283411546
32	17.060329923173917	31.13653203686612	32.78328805230933	19.01984998765063
33	16.952955399274636	30.769690745771967	34.039283996516176	18.23806985843722
34	17.107907496717665	31.21244816514358	35.046472629895874	16.63317170824288
35	18.860218128875427	31.287324346458327	32.18973832334551	17.66271920132073
36	17.359574661692253	30.895784314999936	33.55700859256178	18.18763243074603
37	18.69486656180535	30.912423466403215	30.63657753454574	19.7561324372457
38	18.653788656778506	32.83788526785134	29.526954125339604	18.98137195003055
39	18.97565224173567	30.234638033460293	31.473994826263862	19.315714898540172
40	21.17514006785654	30.159501865404863	31.006538666528005	17.65881940021059
41	19.188061408868148	29.1060355921848	32.974378306706356	18.731524692240693
42	19.480286505388225	32.60155732057665	31.421217517906584	16.49693865612854
43	17.137285998414082	32.10706253981047	30.85314648952903	19.902504972246415
44	16.948535624683142	31.649225889479638	32.41228697336436	18.989951512472864
45	17.82755079490946	31.954450323033523	30.64203725609994	19.575961625957074
46	18.356103838704225	29.45571775839432	31.154211135232103	21.033967267669347
47	21.324112470264016	29.365242372638996	30.168861388069207	19.14178376902778
48	17.33357598762463	33.7678578392502	27.65582955269281	21.24273662043236
49	18.623370208119386	30.040947911656506	31.374159917844192	19.96152196237992
50	16.478999571021877	35.371456055741156	26.816072380308604	21.33347199292836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	83.0
2	78.0
3	235.5
4	393.0
5	441.0
6	489.0
7	418.0
8	347.0
9	489.0
10	631.0
11	932.5
12	1234.0
13	2072.0
14	2910.0
15	4217.5
16	5525.0
17	6502.0
18	7479.0
19	8130.0
20	8781.0
21	9206.5
22	9632.0
23	9587.5
24	9543.0
25	8943.5
26	8344.0
27	9626.5
28	10909.0
29	12520.0
30	14131.0
31	16321.0
32	18511.0
33	22453.0
34	26395.0
35	29549.5
36	32704.0
37	32867.5
38	33031.0
39	29250.0
40	25469.0
41	23658.0
42	21847.0
43	20645.0
44	19443.0
45	21176.5
46	22910.0
47	24172.5
48	25435.0
49	21519.0
50	17603.0
51	14286.5
52	10970.0
53	9392.0
54	7814.0
55	7335.5
56	6857.0
57	6832.0
58	6807.0
59	6293.5
60	5780.0
61	5421.5
62	5063.0
63	4718.0
64	4373.0
65	3965.0
66	3557.0
67	3280.5
68	3004.0
69	2657.5
70	2311.0
71	1980.5
72	1650.0
73	1385.0
74	1120.0
75	897.5
76	675.0
77	543.0
78	411.0
79	305.0
80	199.0
81	152.5
82	106.0
83	67.5
84	29.0
85	24.5
86	20.0
87	18.0
88	16.0
89	11.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	1.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013779297255839951
2	0.004939748072848285
3	5.19973481352451E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	384635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.90119677967859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46222041745747	37.879952836249245
2	12.508415726445241	12.233529976818499
3	4.262576125096896	6.2533522164516935
4	1.818982125789779	3.558016114878562
5	1.0087651878431396	2.4664912477603407
6	0.5831686598789597	1.7110587235489478
7	0.3840140855541427	1.3145123854696021
8	0.2685552839164558	1.0506139828016847
9	0.210245130882297	0.9253114665520037
>10	1.2158239176115095	11.602888323973115
>50	0.1495671195891018	5.13312975163199
>100	0.11591451768155364	10.666962828234881
>500	0.009615029116442238	3.312463405447314
>1k	0.002136673136987164	1.8917167401821189
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2289	0.5951096494078801	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1967	0.5113939189101355	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1847	0.4801955100289885	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1139	0.2961248976302208	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	993	0.25816683349149194	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	912	0.23710790749671765	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	903	0.23476802683063164	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	877	0.2280083715730498	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	866	0.22514851742561132	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	757	0.1968099626919027	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	756	0.1965499759512265	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	659	0.17133126210563263	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	658	0.1710712753649564	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	643	0.16717147425481302	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	637	0.16561155381075565	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	633	0.16457160684805075	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	630	0.16379164662602205	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	614	0.15963185877520245	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	555	0.14429264107530515	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	535	0.13909290626178064	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	534	0.1388329195211044	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	519	0.13493311841096103	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	462	0.12011387419241619	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	447	0.1162140730822728	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	434	0.11283424545348186	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	431	0.1120542852314532	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	423	0.10997439130604339	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	410	0.10659456367725247	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	406	0.10555461671454755	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	404	0.1050346432331951	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	403	0.1047746564925189	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.599867406762255E-4	0.0
10	0.0	0.0	0.0	2.599867406762255E-4	0.0
11	0.0	0.0	0.0	2.599867406762255E-4	0.0
12	0.0	0.0	0.0	5.19973481352451E-4	0.0
13	0.0	0.0	0.0	5.19973481352451E-4	0.0
14	0.0	0.0	0.0	5.19973481352451E-4	0.0
15	0.0	0.0	0.0	0.0012999337033811275	0.0
16	0.0	0.0	0.0	0.0023398806660860296	0.0
17	0.0	0.0	0.0	0.002599867406762255	0.0
18	0.0	0.0	0.0	0.0028598541474384805	0.0
19	0.0	0.0	0.0	0.003379827628790932	0.0
20	0.0	0.0	0.0	0.004159787850819608	0.0
21	0.0	0.0	0.0	0.008839549182991667	0.0
22	0.0	0.0	0.0	0.012999337033811276	0.0
23	0.0	0.0	0.0	0.027558594511679906	0.0
24	0.0	0.0	0.0	0.04029794480481495	0.0
25	0.0	0.0	0.0	0.05303729509795001	0.0
26	0.0	0.0	0.0	0.06863649953852353	0.0
27	0.0	0.0	0.0	0.08709555812653555	0.0
28	0.0	0.0	0.0	0.1182939670076826	0.0
29	0.0	0.0	0.0	0.17055130188360393	0.0
30	0.0	0.0	0.0	0.2589467937135206	0.0
31	0.0	0.0	0.0	0.4105190635277601	0.0
32	0.0	0.0	0.0	0.6161685754026545	0.0
33	0.0	0.0	0.0	0.7685208054389226	0.0
34	0.0	0.0	0.0	0.9583111261325672	0.0
35	0.0	0.0	0.0	1.1733201606718058	0.0
36	0.0	0.0	0.0	1.4509859997140147	0.0
37	0.0	0.0	0.0	1.8570852886502789	0.0
38	0.0	0.0	0.0	2.2683843124000678	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACAGC	25	0.002350839	35.199898	6
GTTGCTA	25	0.002350839	35.199898	44
GAAGCGG	40	1.8169007E-5	32.9999	9
TCGTATA	40	1.8169007E-5	32.9999	44
ATACATG	75	6.184564E-11	32.270767	1
TAGGACC	375	0.0	32.266575	4
GAGTTAT	35	3.2138705E-4	31.428478	13
AGGTGCG	30	0.00574029	29.333246	7
CCATTAG	30	0.00574029	29.333246	18
GGAAGCG	55	4.9496284E-6	27.999916	8
TATGCGT	60	9.7306565E-6	25.66659	39
GTCCTAT	70	1.1276643E-6	25.146051	1
AGGACCT	710	0.0	24.788658	5
GATAATA	45	0.0013954495	24.44755	1
GAGTGTG	65	1.8092656E-5	23.692238	7
GCGTTTA	65	1.8092656E-5	23.692238	42
ATGCGTT	65	1.8092656E-5	23.692238	40
TATATGG	75	2.0582338E-6	23.466599	14
GGACCTG	770	0.0	23.428501	6
TGTAGGA	1170	0.0	23.128136	2
>>END_MODULE