FastQCFastQC Report
Thu 2 Feb 2017
SRR4062521_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062521_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433065
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG47610.33222498630557584No Hit
TCCATGTACTCTGCGTTGATACCAC40830.2849138036306797No Hit
GAGTACATGGAAGCAGTGGTATCAA22130.15442425849490427No Hit
GTATCAACGCAGAGTACTTTTTTTT21380.14919072058838923No Hit
CATGTACTCTGCGTTGATACCACTG18950.13223405777128044No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17030.1188362007306019No Hit
GCGCAAGACGGACCAGAGCGAAAGC15370.10725263683084856No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15210.10613614874412536No Hit
GAATAACGCCGCCGCATCGCCAGTC15210.10613614874412536No Hit
TATCAACGCAGAGTACTTTTTTTTT15110.10543834368992334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT755.8778824E-815.1928762
CGTCTTA1006.5847416E-1014.2522815
GCGTTAT852.7174065E-713.4040761
TCGCGTA1301.4551915E-1113.1550319
TACCGTG655.447294E-513.1550317
CGCATCG2550.013.03993513
GCATCGC2800.012.2149614
CAAGACG3350.012.1917214
GCGCAAG3550.012.0353491
GTCCTAG1603.6379788E-1211.8681921
CGCGTAA1459.640644E-1111.79416710
TAGGACA1052.7257738E-711.7596784
GTACCGT658.0166804E-411.693366
TAAGGCC658.035499E-411.6900944
TCGAACG658.0496375E-411.6876443
CGCAAGA3500.011.6659592
ATACGCT1800.011.6121578
CGCCAGT2950.011.59588518
AAGACGG3650.011.4514865
CGAGCCG3350.011.34509815
Produced by FastQC (version 0.11.5)