##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062521_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1433065
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178956990785483	32.0	32.0	32.0	32.0	32.0
2	30.739106739750117	32.0	32.0	32.0	32.0	32.0
3	30.71955703335159	32.0	32.0	32.0	32.0	32.0
4	30.821590786182064	32.0	32.0	32.0	32.0	32.0
5	30.532057513092568	32.0	32.0	32.0	32.0	32.0
6	34.232461891121474	36.0	36.0	36.0	32.0	36.0
7	34.11004246143755	36.0	36.0	36.0	32.0	36.0
8	34.08787807950093	36.0	36.0	36.0	32.0	36.0
9	34.317206128123985	36.0	36.0	36.0	32.0	36.0
10	33.881793917233345	36.0	36.0	36.0	32.0	36.0
11	34.295096174981595	36.0	36.0	36.0	32.0	36.0
12	34.01213622550268	36.0	36.0	36.0	32.0	36.0
13	34.16700428801206	36.0	36.0	36.0	32.0	36.0
14	34.04190807814021	36.0	36.0	36.0	32.0	36.0
15	33.962437153932306	36.0	36.0	36.0	32.0	36.0
16	33.961085505542314	36.0	36.0	36.0	32.0	36.0
17	33.873167651153295	36.0	36.0	36.0	32.0	36.0
18	33.890534623342276	36.0	36.0	36.0	32.0	36.0
19	33.88511826051156	36.0	36.0	36.0	32.0	36.0
20	33.88550414670654	36.0	36.0	36.0	32.0	36.0
21	33.865939786401874	36.0	36.0	36.0	32.0	36.0
22	33.808564859235275	36.0	36.0	36.0	32.0	36.0
23	33.74758437335362	36.0	36.0	36.0	32.0	36.0
24	33.72312909742405	36.0	36.0	36.0	27.0	36.0
25	33.22191526553227	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	53.0
5	130.0
6	249.0
7	54.0
8	220.0
9	268.0
10	168.0
11	55.0
12	111.0
13	85.0
14	206.0
15	248.0
16	439.0
17	596.0
18	829.0
19	1104.0
20	1781.0
21	2807.0
22	4288.0
23	6506.0
24	9283.0
25	13134.0
26	18526.0
27	24190.0
28	32612.0
29	43210.0
30	56701.0
31	76533.0
32	109685.0
33	154924.0
34	325967.0
35	548095.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.00818461727423	17.08057325818671	10.957603012564448	25.953639111974606
2	17.227189241092862	19.45585788774382	36.18088663845561	27.1360662327077
3	19.555306503129476	22.97780789358173	27.98043527261587	29.486450330672927
4	12.978691627397994	14.720796949326665	34.994528440940655	37.305982982334676
5	14.967034424507261	36.03540527239792	33.47969187919487	15.517868423899955
6	35.5535139459698	34.16621230769445	16.381357156869385	13.898916589466365
7	30.933577449682424	29.952433622139175	20.43978649595281	18.67420243222559
8	28.812441856891823	32.33622849927225	19.288845183585554	19.562484460250367
9	27.01619493714386	14.527667456879389	18.325857278362097	40.130280327614656
10	15.905267452681235	26.42291645125322	31.497405257671744	26.174410838393804
11	37.89636947325399	21.00738122102792	21.316044112664052	19.780205193054037
12	24.51646803658073	23.46967723542696	28.31422031169644	23.699634416295865
13	29.60075903502502	19.002075748397775	25.106145430943112	26.29101978563409
14	23.574021219288102	19.212224322181036	24.65392018836747	32.55983427016339
15	25.60127986139492	26.468609513030906	22.203429497591525	25.726681127982648
16	26.555611056192646	25.49903417247968	23.14625737469567	24.799097396632003
17	24.46325753367806	25.63990238060803	25.07152723484618	24.82531285086772
18	25.568675293132852	24.354709445442456	25.84648858946168	24.230126671963006
19	25.48811079356872	25.56175888982832	24.843864638432567	24.106265678170395
20	25.388861679614166	24.191063604726633	25.15501717677652	25.265057538882672
21	26.390042769471776	24.41989405804099	24.05026408629078	25.13979908619645
22	25.359645861346948	24.530070195939203	24.628752659254765	25.481531283459084
23	24.355688113913224	24.14478428829393	25.169673455468406	26.329854142324443
24	24.533703593969243	25.35288428749518	24.720417814996395	25.392994303539183
25	25.233574331873648	24.433786192579426	24.510438961127893	25.822200514419038
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	111.0
1	111.0
2	343.5
3	576.0
4	576.0
5	576.0
6	1530.5
7	2485.0
8	2485.0
9	2485.0
10	2795.0
11	3105.0
12	3105.0
13	3105.0
14	3462.5
15	3820.0
16	3820.0
17	3820.0
18	6256.0
19	8692.0
20	8692.0
21	8692.0
22	13586.5
23	18481.0
24	18481.0
25	18481.0
26	27408.5
27	36336.0
28	36336.0
29	36336.0
30	44821.0
31	53306.0
32	53306.0
33	53306.0
34	67551.5
35	81797.0
36	81797.0
37	81797.0
38	94790.5
39	107784.0
40	107784.0
41	107784.0
42	125393.5
43	143003.0
44	143003.0
45	143003.0
46	171105.5
47	199208.0
48	199208.0
49	199208.0
50	197165.0
51	195122.0
52	195122.0
53	195122.0
54	182879.0
55	170636.0
56	170636.0
57	170636.0
58	157665.0
59	144694.0
60	144694.0
61	144694.0
62	127513.5
63	110333.0
64	110333.0
65	110333.0
66	91425.5
67	72518.0
68	72518.0
69	72518.0
70	55094.0
71	37670.0
72	37670.0
73	37670.0
74	29217.0
75	20764.0
76	20764.0
77	20764.0
78	16895.0
79	13026.0
80	13026.0
81	13026.0
82	9165.5
83	5305.0
84	5305.0
85	5305.0
86	3916.5
87	2528.0
88	2528.0
89	2528.0
90	1737.0
91	946.0
92	946.0
93	946.0
94	550.5
95	155.0
96	155.0
97	155.0
98	409.5
99	664.0
100	664.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009280807220886702
2	0.0039774888089514435
3	0.0054428794227756585
4	0.014025881589460353
5	0.035588057764302385
6	0.04493864549060929
7	0.06705906570881294
8	0.08743497329151155
9	0.09650643899613766
10	0.1192548837631231
11	0.12072027437694732
12	0.13460659495556726
13	0.12379061661543615
14	0.12700051986476538
15	0.11583563899753326
16	0.11513783394333126
17	0.12504666571299974
18	0.13174559423333904
19	0.13502527798808847
20	0.12365105560459574
21	0.13286208232006225
22	0.15442425849490427
23	0.14521323177943776
24	0.1396307913458217
25	0.13509505849350867
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1433065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.53052579271749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07929957097943	45.0237613608174
2	11.639990180910676	12.927495499360772
3	3.181223605143847	5.299650583735263
4	1.3391595843412787	2.974569433553128
5	0.7109372884007866	1.9739360715272256
6	0.4278246883647309	1.425439793519941
7	0.2968177338349288	1.1537711377119526
8	0.20559596047625323	0.9133481428884084
9	0.15374884539950182	0.7683978802551813
>10	0.7891466283913166	8.014908710342988
>50	0.08035246558679206	3.1322009613041906
>100	0.08320854593219026	9.86324491463936
>500	0.009049831298621379	3.3650763357569295
>1k	0.0036450709397224997	3.1641991745873357
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4761	0.33222498630557584	No Hit
TCCATGTACTCTGCGTTGATACCAC	4083	0.2849138036306797	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2213	0.15442425849490427	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2138	0.14919072058838923	No Hit
CATGTACTCTGCGTTGATACCACTG	1895	0.13223405777128044	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1703	0.1188362007306019	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1537	0.10725263683084856	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1521	0.10613614874412536	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1521	0.10613614874412536	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1511	0.10543834368992334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.978050542020075E-5
2	0.0	0.0	0.0	0.0	6.978050542020075E-5
3	0.0	0.0	0.0	0.0	6.978050542020075E-5
4	0.0	0.0	0.0	6.978050542020075E-5	6.978050542020075E-5
5	0.0	0.0	0.0	6.978050542020075E-5	6.978050542020075E-5
6	0.0	0.0	0.0	6.978050542020075E-5	6.978050542020075E-5
7	0.0	0.0	0.0	6.978050542020075E-5	6.978050542020075E-5
8	0.0	0.0	0.0	6.978050542020075E-5	6.978050542020075E-5
9	0.0	0.0	0.0	6.978050542020075E-5	6.978050542020075E-5
10	0.0	0.0	0.0	6.978050542020075E-5	6.978050542020075E-5
11	0.0	0.0	0.0	1.395610108404015E-4	6.978050542020075E-5
12	0.0	0.0	0.0	1.395610108404015E-4	2.79122021680803E-4
13	0.0	0.0	0.0	2.093415162606023E-4	2.79122021680803E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	75	5.8778824E-8	15.192876	2
CGTCTTA	100	6.5847416E-10	14.25228	15
GCGTTAT	85	2.7174065E-7	13.404076	1
TCGCGTA	130	1.4551915E-11	13.155031	9
TACCGTG	65	5.447294E-5	13.155031	7
CGCATCG	255	0.0	13.039935	13
GCATCGC	280	0.0	12.21496	14
CAAGACG	335	0.0	12.191721	4
GCGCAAG	355	0.0	12.035349	1
GTCCTAG	160	3.6379788E-12	11.868192	1
CGCGTAA	145	9.640644E-11	11.794167	10
TAGGACA	105	2.7257738E-7	11.759678	4
GTACCGT	65	8.0166804E-4	11.69336	6
TAAGGCC	65	8.035499E-4	11.690094	4
TCGAACG	65	8.0496375E-4	11.687644	3
CGCAAGA	350	0.0	11.665959	2
ATACGCT	180	0.0	11.612157	8
CGCCAGT	295	0.0	11.595885	18
AAGACGG	365	0.0	11.451486	5
CGAGCCG	335	0.0	11.345098	15
>>END_MODULE