FastQCFastQC Report
Thu 2 Feb 2017
SRR4062521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433065
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17710.12358127509917555No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC17320.1208598353877877No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16490.11506805343791104No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15770.1100438570476566No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14860.10369383105441833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT300.00574556529.3331246
TAGTACG801.3098179E-724.7506872
ATACCGA654.9330137E-420.3075496
CGCAATA6400.020.28110536
GCCGGTC6500.019.9690888
TAGCGGC6700.019.70135130
CTAGCGG6700.019.70135129
TCTAGCG6600.019.66652728
CGCCGGT6650.019.5186587
ATACGAA6450.019.44172340
CGAATGC6600.019.33319543
TACGAAT6700.019.0446441
CGGTCCA7400.018.72959510
CAATACG6950.018.67612538
AATACGA7000.018.54272539
GTATTAC951.5937083E-518.5313571
CTTGCGC7650.017.8299393
GTATTAG3500.017.6047881
CCGGTCC8000.017.5998749
GCAATAC7500.017.59987437
Produced by FastQC (version 0.11.5)