##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062521_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1433065
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266536409723216	32.0	32.0	32.0	32.0	32.0
2	31.388489007825882	32.0	32.0	32.0	32.0	32.0
3	31.490850031226778	32.0	32.0	32.0	32.0	32.0
4	31.599780191407927	32.0	32.0	32.0	32.0	32.0
5	31.534510297858088	32.0	32.0	32.0	32.0	32.0
6	35.13334566122263	36.0	36.0	36.0	36.0	36.0
7	35.14444843744003	36.0	36.0	36.0	36.0	36.0
8	35.0830074002226	36.0	36.0	36.0	36.0	36.0
9	35.22691015411024	36.0	36.0	36.0	36.0	36.0
10	35.08324186272081	36.0	36.0	36.0	36.0	36.0
11	35.22508190486824	36.0	36.0	36.0	36.0	36.0
12	35.132948610146784	36.0	36.0	36.0	36.0	36.0
13	35.180825014915584	36.0	36.0	36.0	36.0	36.0
14	35.143160289309975	36.0	36.0	36.0	36.0	36.0
15	35.09227983378283	36.0	36.0	36.0	36.0	36.0
16	35.1081248931486	36.0	36.0	36.0	36.0	36.0
17	35.08411621245373	36.0	36.0	36.0	36.0	36.0
18	35.08962677896676	36.0	36.0	36.0	36.0	36.0
19	35.07754568006336	36.0	36.0	36.0	36.0	36.0
20	35.07659666518965	36.0	36.0	36.0	36.0	36.0
21	35.06508288179531	36.0	36.0	36.0	36.0	36.0
22	35.04499586550505	36.0	36.0	36.0	36.0	36.0
23	34.99917030979056	36.0	36.0	36.0	36.0	36.0
24	34.97538143768775	36.0	36.0	36.0	36.0	36.0
25	34.9472571027832	36.0	36.0	36.0	32.0	36.0
26	34.897620833667695	36.0	36.0	36.0	32.0	36.0
27	34.87415783652521	36.0	36.0	36.0	32.0	36.0
28	34.859666518964595	36.0	36.0	36.0	32.0	36.0
29	34.828942860233134	36.0	36.0	36.0	32.0	36.0
30	34.81922732046348	36.0	36.0	36.0	32.0	36.0
31	34.81688758011674	36.0	36.0	36.0	32.0	36.0
32	34.77322103323994	36.0	36.0	36.0	32.0	36.0
33	34.75000645469675	36.0	36.0	36.0	32.0	36.0
34	34.735051096775095	36.0	36.0	36.0	32.0	36.0
35	34.695853293465404	36.0	36.0	36.0	32.0	36.0
36	34.67868798693709	36.0	36.0	36.0	32.0	36.0
37	34.658725877751536	36.0	36.0	36.0	32.0	36.0
38	34.61565874541629	36.0	36.0	36.0	32.0	36.0
39	34.587333442656124	36.0	36.0	36.0	32.0	36.0
40	34.55891254060353	36.0	36.0	36.0	32.0	36.0
41	34.54179817384417	36.0	36.0	36.0	32.0	36.0
42	34.482218880511354	36.0	36.0	36.0	32.0	36.0
43	34.49035040280797	36.0	36.0	36.0	32.0	36.0
44	34.46544922944877	36.0	36.0	36.0	32.0	36.0
45	34.39599599459899	36.0	36.0	36.0	32.0	36.0
46	34.43647496798819	36.0	36.0	36.0	32.0	36.0
47	34.40137607156689	36.0	36.0	36.0	32.0	36.0
48	34.37286794388252	36.0	36.0	36.0	32.0	36.0
49	34.38176286490843	36.0	36.0	36.0	32.0	36.0
50	33.846570811512386	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	40.0
22	116.0
23	334.0
24	980.0
25	2266.0
26	4809.0
27	9146.0
28	15347.0
29	22867.0
30	32641.0
31	46933.0
32	69814.0
33	110458.0
34	247576.0
35	869727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40655561968585	17.652547690018544	11.487060376997684	26.45383631329793
2	16.57965824216697	19.983768352194655	35.9300015422159	27.50657186342248
3	18.919122603551287	23.217064487271273	28.339676412259657	29.52413649691778
4	12.700575900118697	15.271436456647693	35.17582294124256	36.85216470199105
5	14.679515583731373	36.26765010658972	33.36980527749962	15.68302903217928
6	35.697294113788594	34.42477326505989	16.100361394246303	13.777571226905206
7	30.899575385624516	30.025574555236506	20.37869880291543	18.696151256223548
8	28.744683597743297	32.63348138430567	19.126208511128244	19.495626506822788
9	27.079860299428148	14.340521888400037	18.303147449696976	40.27647036247484
10	15.833894484897753	26.54862131166416	31.47770687303088	26.139777330407206
11	38.19254534860596	20.91810210981358	21.192827959652913	19.696524581927548
12	24.41515213894694	23.505493470289203	28.293761971717963	23.78559241904589
13	29.779040029586934	18.8797437659841	25.128797367879336	26.212418836549634
14	23.54436121180826	19.192639552288277	24.53594219382931	32.72705704207415
15	25.595559168635056	26.571509317441986	22.22864978211037	25.604281731812584
16	26.533409161482556	25.4285046386591	23.223998911424115	24.81408728843423
17	24.575089057370043	25.726955860341295	24.939761978696012	24.75819310359265
18	25.743772962147567	24.22576784723652	25.81466995565449	24.21578923496143
19	25.544200716645793	25.506519243718884	24.854141298545425	24.0951387410899
20	25.51600939245603	24.082089786576326	25.235421980161405	25.166478840806246
21	26.487633149926904	24.51089099238346	23.979582224114047	25.021893633575587
22	25.505123633610477	24.45534571006898	24.596023208996105	25.443507447324443
23	24.368329419809985	24.013355988737427	25.208068022036684	26.4102465694159
24	24.71018411586355	25.22725766102724	24.69183184293804	25.37072638017117
25	25.340302079807962	24.191226497053517	24.62735465592977	25.841116767208742
26	24.71667370286763	24.849535785187694	25.198508092794114	25.23528241915056
27	25.806086953487807	24.669990544741516	24.40028889129244	25.12363361047824
28	24.90061511515528	24.05675946310879	25.296340361393238	25.746285060342693
29	24.596721014050303	24.944716394580844	25.531570445164736	24.926992146204118
30	25.149382616978293	24.646265172898644	25.49995987620938	24.704392333913674
31	25.080858160655655	24.74081775774302	24.50802999166123	25.670294089940093
32	24.93955263717975	24.613956798889095	24.551642807548856	25.8948477563823
33	24.60453643065737	24.248446511498084	25.14435842058804	26.00265863725651
34	25.16201288845935	24.512775066029803	25.485933994619923	24.839278050890922
35	26.018568592492315	24.258704245794853	25.064320180871068	24.65840698084176
36	24.884216696381532	24.968581327434556	24.89056672237477	25.256635253809144
37	26.039153841591272	24.519543775055563	24.7276292422186	24.713673141134564
38	24.84981490720937	24.346348560602625	25.080020794590613	25.723815737597388
39	25.247354446588254	24.469371591658437	24.684714231385176	25.598559730368127
40	25.576648651666183	24.543408707909272	24.980095110828888	24.899847529595657
41	24.36204917432217	24.794618527421992	25.583417360691946	25.25991493756389
42	26.07830070513201	24.78610530576073	24.85679295775139	24.278801031355872
43	25.23779451734569	23.813295279697712	24.962370862452158	25.986539340504443
44	24.901940944758262	24.34481338948338	25.308482169336354	25.444763496422006
45	25.069832840799265	24.665873494921726	25.032849172926557	25.231444491352452
46	24.67288597919282	24.42307142114478	25.45170728711857	25.452335312543834
47	25.03489027705671	24.20457146366108	25.70066654385289	25.059871715429317
48	25.654105012682603	25.41091995129321	24.35507112378015	24.579903912244035
49	24.47955954544979	24.807946603957255	25.350490033599314	25.362003816993646
50	24.465411174937056	25.488184756577514	24.619905321744177	25.42649874674125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	18.5
2	23.0
3	93.5
4	164.0
5	196.0
6	228.0
7	199.0
8	170.0
9	219.0
10	268.0
11	441.5
12	615.0
13	1042.0
14	1469.0
15	1977.0
16	2485.0
17	2828.0
18	3171.0
19	3426.5
20	3682.0
21	4478.5
22	5275.0
23	5692.5
24	6110.0
25	7378.0
26	8646.0
27	10681.0
28	12716.0
29	14475.5
30	16235.0
31	17660.5
32	19086.0
33	22040.5
34	24995.0
35	28930.5
36	32866.0
37	40342.5
38	47819.0
39	53046.5
40	58274.0
41	65063.5
42	71853.0
43	75049.5
44	78246.0
45	87785.0
46	97324.0
47	105437.5
48	113551.0
49	114706.0
50	115861.0
51	110433.0
52	105005.0
53	103348.5
54	101692.0
55	102786.5
56	103881.0
57	100641.0
58	97401.0
59	89365.5
60	81330.0
61	72158.0
62	62986.0
63	54745.5
64	46505.0
65	39773.0
66	33041.0
67	28772.5
68	24504.0
69	22593.0
70	20682.0
71	16119.0
72	11556.0
73	10477.5
74	9399.0
75	6867.0
76	4335.0
77	3915.0
78	3495.0
79	3044.5
80	2594.0
81	2019.5
82	1445.0
83	1230.5
84	1016.0
85	831.0
86	646.0
87	465.5
88	285.0
89	179.0
90	73.0
91	47.0
92	21.0
93	18.5
94	16.0
95	11.0
96	6.0
97	4.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015212150181603766
2	0.004326391336052447
3	4.884635379414052E-4
4	2.79122021680803E-4
5	0.0
6	2.79122021680803E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.395610108404015E-4
47	6.978050542020075E-5
48	0.0
49	0.0
50	6.978050542020075E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1433065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.34652807105307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.51828339060813	47.563088101541766
2	11.549886511846415	13.477915551618484
3	3.0374564092945358	5.316751069485112
4	1.2850961054534589	2.999235839633649
5	0.6818200611513483	1.989091666868713
6	0.41726323551435934	1.46075166503742
7	0.27218068599176115	1.1116558624931743
8	0.18914006346528467	0.8828532817870393
9	0.15028076395700946	0.7891524731481256
>10	0.7418490540149919	7.8502676850955355
>50	0.07305204971313513	2.9807065873042915
>100	0.0739517458302097	9.009192350374839
>500	0.00793623368541837	3.1232835917492006
>1k	0.0018036894739587202	1.4460542738626316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1771	0.12358127509917555	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1732	0.1208598353877877	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1649	0.11506805343791104	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1577	0.1100438570476566	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1486	0.10369383105441833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	6.978050542020075E-5	0.0
12	0.0	0.0	0.0	6.978050542020075E-5	2.79122021680803E-4
13	0.0	0.0	0.0	1.395610108404015E-4	3.489025271010038E-4
14	0.0	0.0	0.0	1.395610108404015E-4	3.489025271010038E-4
15	0.0	0.0	0.0	2.093415162606023E-4	3.489025271010038E-4
16	0.0	0.0	0.0	2.79122021680803E-4	3.489025271010038E-4
17	0.0	0.0	0.0	3.489025271010038E-4	3.489025271010038E-4
18	0.0	0.0	0.0	4.186830325212046E-4	3.489025271010038E-4
19	0.0	0.0	0.0	5.58244043361606E-4	3.489025271010038E-4
20	0.0	0.0	0.0	6.280245487818068E-4	4.186830325212046E-4
21	0.0	0.0	0.0	8.373660650424092E-4	4.186830325212046E-4
22	0.0	0.0	0.0	0.0013258296029838145	4.186830325212046E-4
23	0.0	0.0	0.0	0.002232976173446424	4.186830325212046E-4
24	0.0	0.0	0.0	0.0030703422384888335	4.186830325212046E-4
25	0.0	0.0	0.0	0.003768147292690841	4.186830325212046E-4
26	0.0	0.0	0.0	0.0048148548739938525	4.186830325212046E-4
27	0.0	0.0	0.0	0.007047831047440277	4.186830325212046E-4
28	0.0	0.0	0.0	0.012700051986476538	4.186830325212046E-4
29	0.0	0.0	0.0	0.02295778628324605	4.186830325212046E-4
30	0.0	0.0	0.0	0.038937522024472024	4.186830325212046E-4
31	0.0	0.0	0.0	0.07522338484297642	4.186830325212046E-4
32	0.0	0.0	0.0	0.11778949314929887	4.186830325212046E-4
33	0.0	0.0	0.0	0.15833196679843553	4.186830325212046E-4
34	0.0	0.0	0.0	0.20675963756005486	4.186830325212046E-4
35	0.0	0.0	0.0	0.2664219696943265	4.186830325212046E-4
36	0.0	0.0	0.0	0.35043769822024823	4.186830325212046E-4
37	0.0	0.0	0.0	0.47283270472728034	4.186830325212046E-4
38	0.0	0.0	0.0	0.6293503783847907	4.884635379414054E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGT	30	0.005745565	29.333124	6
TAGTACG	80	1.3098179E-7	24.750687	2
ATACCGA	65	4.9330137E-4	20.307549	6
CGCAATA	640	0.0	20.281105	36
GCCGGTC	650	0.0	19.969088	8
TAGCGGC	670	0.0	19.701351	30
CTAGCGG	670	0.0	19.701351	29
TCTAGCG	660	0.0	19.666527	28
CGCCGGT	665	0.0	19.518658	7
ATACGAA	645	0.0	19.441723	40
CGAATGC	660	0.0	19.333195	43
TACGAAT	670	0.0	19.04464	41
CGGTCCA	740	0.0	18.729595	10
CAATACG	695	0.0	18.676125	38
AATACGA	700	0.0	18.542725	39
GTATTAC	95	1.5937083E-5	18.531357	1
CTTGCGC	765	0.0	17.829939	3
GTATTAG	350	0.0	17.604788	1
CCGGTCC	800	0.0	17.599874	9
GCAATAC	750	0.0	17.599874	37
>>END_MODULE