FastQCFastQC Report
Thu 2 Feb 2017
SRR4062520_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062520_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066665
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG24940.2338128653326021No Hit
TCCATGTACTCTGCGTTGATACCAC21100.19781280908251422No Hit
GTATCAACGCAGAGTACTTTTTTTT19730.18496903901412345No Hit
TATCAACGCAGAGTACTTTTTTTTT13560.12712519863312285No Hit
GTCTTGCGCCGGTCCAAGAATTTCA12750.11953143676786994No Hit
GAATAGGACCGCGGTTCTATTTTGT12070.11315642680691688No Hit
GGGTAGGCACACGCTGAGCCAGTCA11930.11184392475613242No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11910.11165642446316322No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11900.11156267431667861No Hit
GAGTACATGGAAGCAGTGGTATCAA11740.11006267197292496No Hit
GCGCAAGACGGACCAGAGCGAAAGC11630.10903142036159431No Hit
GAATAACGCCGCCGCATCGCCAGTC10820.10143765849634141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA753.268724E-916.47036716
TATGCCG350.002169740616.2855535
CGTCAAT903.6259735E-813.72466419
CCGTCCA707.254641E-613.5719329
GCATCGC2050.012.97791814
CGGTTCT2050.012.976712
CAAGACG2050.012.9754814
ATCGCCA2100.012.66951516
ATCCCCG1352.7284841E-1112.66773112
CGCATCG2100.012.6677313
GTTCTAA905.440361E-712.6588181
CGGTCCA3100.012.56498110
CGCCAGT2050.012.514420518
CCGCATC2250.012.24547412
GAATTTC3350.012.19619818
ACCGTCG1251.4078978E-912.160458
AGTACCT550.003067584712.090795
TCGCCAG2150.011.93291417
ATACCGT1459.640644E-1111.793546
CATCGCC2350.011.7254915
Produced by FastQC (version 0.11.5)