##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062520_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1066665
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14034115678306	32.0	32.0	32.0	32.0	32.0
2	30.72016612525957	32.0	32.0	32.0	32.0	32.0
3	30.701247345698977	32.0	32.0	32.0	32.0	32.0
4	30.79231998799998	32.0	32.0	32.0	32.0	32.0
5	30.526007696887028	32.0	32.0	32.0	32.0	32.0
6	34.21037532871145	36.0	36.0	36.0	32.0	36.0
7	34.07066136040837	36.0	36.0	36.0	32.0	36.0
8	34.057363839631	36.0	36.0	36.0	32.0	36.0
9	34.25779415280336	36.0	36.0	36.0	32.0	36.0
10	33.853300708282354	36.0	36.0	36.0	32.0	36.0
11	34.26360759938687	36.0	36.0	36.0	32.0	36.0
12	33.98001715627681	36.0	36.0	36.0	32.0	36.0
13	34.12515175804962	36.0	36.0	36.0	32.0	36.0
14	34.019672530738326	36.0	36.0	36.0	32.0	36.0
15	33.94168647138511	36.0	36.0	36.0	32.0	36.0
16	33.945613665021355	36.0	36.0	36.0	32.0	36.0
17	33.85378070903236	36.0	36.0	36.0	32.0	36.0
18	33.8743569911828	36.0	36.0	36.0	32.0	36.0
19	33.8623241598815	36.0	36.0	36.0	32.0	36.0
20	33.86504666413541	36.0	36.0	36.0	32.0	36.0
21	33.83402286566073	36.0	36.0	36.0	32.0	36.0
22	33.78437560058688	36.0	36.0	36.0	32.0	36.0
23	33.73891146704917	36.0	36.0	36.0	32.0	36.0
24	33.69821265345727	36.0	36.0	36.0	27.0	36.0
25	33.215212836270055	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	46.0
5	102.0
6	175.0
7	49.0
8	151.0
9	193.0
10	126.0
11	36.0
12	81.0
13	66.0
14	215.0
15	242.0
16	404.0
17	500.0
18	693.0
19	1037.0
20	1596.0
21	2364.0
22	3600.0
23	5260.0
24	7371.0
25	10183.0
26	14356.0
27	18064.0
28	24354.0
29	32484.0
30	41273.0
31	56203.0
32	80086.0
33	112959.0
34	240235.0
35	412155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.37663222894475	16.919394353638744	10.90709128293406	25.796882134482445
2	16.999928747417094	19.446986203249867	37.015529312937595	26.537555736395447
3	19.57326602994713	23.259138975429703	27.982032846027565	29.1855621485956
4	12.677168307548053	14.6070323488045	35.42044069385841	37.29535864978903
5	14.629788886210878	36.49902419307191	33.511113695340036	15.36007322537717
6	35.22459537940919	34.61653898389021	16.4931193865224	13.665746250178207
7	30.783071721534473	30.05152182641685	20.394368998345147	18.771037453703528
8	28.610530128372947	32.86473126694273	19.129642505604178	19.395096099080156
9	27.232496009503997	14.14066627442038	18.418496758337813	40.20834095773781
10	15.926114348361518	26.460423362268344	31.58481137175983	26.028650917610307
11	37.94941204739664	20.86030306215928	21.678593419135865	19.51169147130821
12	24.5424453731971	23.542941041730035	28.71328954927959	23.201324035793274
13	29.74558254135868	19.04841018420744	25.227267394110054	25.978739880323833
14	23.417826079610414	19.4550461919128	24.830147802856935	32.29697992561985
15	25.365877923996504	27.10823867076008	22.188631856368804	25.337251548874605
16	26.003724136118677	25.53830097552094	23.918376847286016	24.539598041074367
17	24.178475147746813	25.916893825046746	25.31314064295208	24.591490384254357
18	25.178456096608485	24.509316140466822	26.408310553035534	23.90391720988915
19	25.55555764168691	25.311761900649245	24.96817606614872	24.16450439151512
20	25.512712568392182	24.161811514009777	25.305271536556084	25.020204381041957
21	26.38880414099588	24.340836103416002	24.309857488986164	24.960502266601956
22	25.531413462630876	24.62036530186275	24.856225652254334	24.991995583252038
23	24.004127036879524	24.41692460647164	25.431221841375706	26.147726515273135
24	24.49036684199706	25.300616676069982	25.143185443102738	25.065831038830222
25	24.848979821732826	24.65447240332127	25.033724319529128	25.46282345541678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	133.0
1	133.0
2	318.0
3	503.0
4	503.0
5	503.0
6	1308.0
7	2113.0
8	2113.0
9	2113.0
10	2237.5
11	2362.0
12	2362.0
13	2362.0
14	2630.0
15	2898.0
16	2898.0
17	2898.0
18	4565.5
19	6233.0
20	6233.0
21	6233.0
22	10115.5
23	13998.0
24	13998.0
25	13998.0
26	21216.5
27	28435.0
28	28435.0
29	28435.0
30	36122.5
31	43810.0
32	43810.0
33	43810.0
34	54251.5
35	64693.0
36	64693.0
37	64693.0
38	74719.5
39	84746.0
40	84746.0
41	84746.0
42	97558.5
43	110371.0
44	110371.0
45	110371.0
46	127900.5
47	145430.0
48	145430.0
49	145430.0
50	143915.0
51	142400.0
52	142400.0
53	142400.0
54	132304.5
55	122209.0
56	122209.0
57	122209.0
58	112427.0
59	102645.0
60	102645.0
61	102645.0
62	91399.5
63	80154.0
64	80154.0
65	80154.0
66	66610.5
67	53067.0
68	53067.0
69	53067.0
70	40387.0
71	27707.0
72	27707.0
73	27707.0
74	21779.0
75	15851.0
76	15851.0
77	15851.0
78	12830.5
79	9810.0
80	9810.0
81	9810.0
82	6900.0
83	3990.0
84	3990.0
85	3990.0
86	2919.0
87	1848.0
88	1848.0
89	1848.0
90	1253.0
91	658.0
92	658.0
93	658.0
94	394.0
95	130.0
96	130.0
97	130.0
98	300.5
99	471.0
100	471.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009562514941429597
2	0.0034687554199303436
3	0.004875007617199402
4	0.015468774169959641
5	0.034500053906334226
6	0.04509382045909447
7	0.06646885385758415
8	0.08831263798849685
9	0.09450014765648071
10	0.1208439388186544
11	0.11934393647490074
12	0.13471896049837576
13	0.12093768896513901
14	0.12440644438506934
15	0.1138126778323091
16	0.1109064232912864
17	0.12459394467803857
18	0.1328439575686837
19	0.13396895932649894
20	0.12206269072295427
21	0.13256270712922988
22	0.15262523847693513
23	0.1400627188479982
24	0.1348127106448604
25	0.13218770654329146
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1066665.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.572099992186885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8709543740774	44.38596662947625
2	12.293732982948251	13.663771172112924
3	3.4714545955707234	5.787480657101788
4	1.4527971808246392	3.229399608046162
5	0.7618553271649491	2.1168950210395403
6	0.4326534904394734	1.4426077819602652
7	0.3201106019397185	1.2452452865687218
8	0.21257895260386314	0.945076704826899
9	0.1674734456278931	0.8376165959822413
>10	0.8435299086769735	8.598140848287738
>50	0.07984224291497768	3.1432758461025974
>100	0.08185487022750854	9.419966403856705
>500	0.008625202669171515	3.257782515834441
>1k	0.00253682431446221	1.9267749288036775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2494	0.2338128653326021	No Hit
TCCATGTACTCTGCGTTGATACCAC	2110	0.19781280908251422	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1973	0.18496903901412345	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1356	0.12712519863312285	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1275	0.11953143676786994	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1207	0.11315642680691688	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1193	0.11184392475613242	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1191	0.11165642446316322	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1190	0.11156267431667861	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1174	0.11006267197292496	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1163	0.10903142036159431	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1082	0.10143765849634141	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8750029296920776E-4
2	0.0	0.0	0.0	0.0	1.8750029296920776E-4
3	0.0	0.0	0.0	0.0	1.8750029296920776E-4
4	0.0	0.0	0.0	0.0	1.8750029296920776E-4
5	0.0	0.0	0.0	0.0	1.8750029296920776E-4
6	0.0	0.0	0.0	0.0	2.8125043945381164E-4
7	0.0	0.0	0.0	0.0	2.8125043945381164E-4
8	0.0	0.0	0.0	0.0	2.8125043945381164E-4
9	0.0	0.0	0.0	0.0	2.8125043945381164E-4
10	0.0	0.0	0.0	9.375014648460388E-5	2.8125043945381164E-4
11	0.0	0.0	0.0	9.375014648460388E-5	2.8125043945381164E-4
12	0.0	0.0	0.0	9.375014648460388E-5	4.687507324230194E-4
13	0.0	0.0	0.0	9.375014648460388E-5	5.625008789076233E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	75	3.268724E-9	16.470367	16
TATGCCG	35	0.0021697406	16.285553	5
CGTCAAT	90	3.6259735E-8	13.724664	19
CCGTCCA	70	7.254641E-6	13.571932	9
GCATCGC	205	0.0	12.977918	14
CGGTTCT	205	0.0	12.9767	12
CAAGACG	205	0.0	12.975481	4
ATCGCCA	210	0.0	12.669515	16
ATCCCCG	135	2.7284841E-11	12.667731	12
CGCATCG	210	0.0	12.66773	13
GTTCTAA	90	5.440361E-7	12.658818	1
CGGTCCA	310	0.0	12.564981	10
CGCCAGT	205	0.0	12.5144205	18
CCGCATC	225	0.0	12.245474	12
GAATTTC	335	0.0	12.196198	18
ACCGTCG	125	1.4078978E-9	12.16045	8
AGTACCT	55	0.0030675847	12.09079	5
TCGCCAG	215	0.0	11.932914	17
ATACCGT	145	9.640644E-11	11.79354	6
CATCGCC	235	0.0	11.72549	15
>>END_MODULE