FastQCFastQC Report
Thu 2 Feb 2017
SRR4062520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066665
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13530.12684394819366906No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12630.1184064350100547No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11750.11015642211940956No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11110.10415641274439491No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11070.10378141215845652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGG852.3521898E-723.2940442
GTATAGG1650.022.6697831
CGCAATA3800.020.8420436
ATTTCGT1601.8189894E-1220.62493542
TCTAGCG3950.020.05056828
TAGCGGC4300.019.95342430
GTCCTAC3200.019.940241
CTAGCGG4100.019.85359629
CGGTCCA4200.019.38089210
CGAGCCG4350.019.2183315
GTAGGAC3400.018.7646473
TAGGACG2250.018.5777174
CGCCGGT4150.018.5541577
TCCTACA3800.018.5262572
CAATACG4300.018.41854738
ACGCGCT851.4306673E-418.1175915
GCCGGTC4750.018.0630998
TCTTGCG4400.017.9999432
CGAATGC4300.017.9069243
CTTACAC1601.1004886E-917.8749453
Produced by FastQC (version 0.11.5)