##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062520_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1066665
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285142945535853	32.0	32.0	32.0	32.0	32.0
2	31.410584391538112	32.0	32.0	32.0	32.0	32.0
3	31.504771413705335	32.0	32.0	32.0	32.0	32.0
4	31.60781407470949	32.0	32.0	32.0	32.0	32.0
5	31.545187101854847	32.0	32.0	32.0	32.0	32.0
6	35.1475617930653	36.0	36.0	36.0	36.0	36.0
7	35.167366511510174	36.0	36.0	36.0	36.0	36.0
8	35.097187964356195	36.0	36.0	36.0	36.0	36.0
9	35.23667411980331	36.0	36.0	36.0	36.0	36.0
10	35.1064364163069	36.0	36.0	36.0	36.0	36.0
11	35.245268195731555	36.0	36.0	36.0	36.0	36.0
12	35.145536789901236	36.0	36.0	36.0	36.0	36.0
13	35.19497780465282	36.0	36.0	36.0	36.0	36.0
14	35.15133148645545	36.0	36.0	36.0	36.0	36.0
15	35.119379561530565	36.0	36.0	36.0	36.0	36.0
16	35.130728954263994	36.0	36.0	36.0	36.0	36.0
17	35.102630160359624	36.0	36.0	36.0	36.0	36.0
18	35.116535182086224	36.0	36.0	36.0	36.0	36.0
19	35.100701719846434	36.0	36.0	36.0	36.0	36.0
20	35.094375459961654	36.0	36.0	36.0	36.0	36.0
21	35.08619482217941	36.0	36.0	36.0	36.0	36.0
22	35.06123572068081	36.0	36.0	36.0	36.0	36.0
23	35.028300356719306	36.0	36.0	36.0	36.0	36.0
24	34.99679749499609	36.0	36.0	36.0	36.0	36.0
25	34.97198558122747	36.0	36.0	36.0	36.0	36.0
26	34.92677551058674	36.0	36.0	36.0	32.0	36.0
27	34.90729704265163	36.0	36.0	36.0	32.0	36.0
28	34.89139514280491	36.0	36.0	36.0	32.0	36.0
29	34.86371259955094	36.0	36.0	36.0	32.0	36.0
30	34.849426014728145	36.0	36.0	36.0	32.0	36.0
31	34.84949257733215	36.0	36.0	36.0	32.0	36.0
32	34.809974078084494	36.0	36.0	36.0	32.0	36.0
33	34.793184364350566	36.0	36.0	36.0	32.0	36.0
34	34.7662030721923	36.0	36.0	36.0	32.0	36.0
35	34.732572082143875	36.0	36.0	36.0	32.0	36.0
36	34.71133954896805	36.0	36.0	36.0	32.0	36.0
37	34.695928899888905	36.0	36.0	36.0	32.0	36.0
38	34.65268758232435	36.0	36.0	36.0	32.0	36.0
39	34.62268472294488	36.0	36.0	36.0	32.0	36.0
40	34.5991759362124	36.0	36.0	36.0	32.0	36.0
41	34.57949590546235	36.0	36.0	36.0	32.0	36.0
42	34.52407175636212	36.0	36.0	36.0	32.0	36.0
43	34.52494644522882	36.0	36.0	36.0	32.0	36.0
44	34.49241326939573	36.0	36.0	36.0	32.0	36.0
45	34.43310973923397	36.0	36.0	36.0	32.0	36.0
46	34.44895913899865	36.0	36.0	36.0	32.0	36.0
47	34.410230953485865	36.0	36.0	36.0	32.0	36.0
48	34.37210276891058	36.0	36.0	36.0	32.0	36.0
49	34.368593700927654	36.0	36.0	36.0	32.0	36.0
50	33.854573835271616	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	17.0
22	74.0
23	246.0
24	614.0
25	1562.0
26	3288.0
27	6138.0
28	10602.0
29	16514.0
30	23618.0
31	34451.0
32	51448.0
33	83617.0
34	183822.0
35	650645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84218106397802	17.429046410384554	11.465560001012651	26.26321252462478
2	16.367389912312362	19.937147662678704	36.695020846087715	27.000441578921215
3	18.90868999862187	23.557018477251017	28.342972428967723	29.19131909515939
4	12.452222402431513	15.075736339849305	35.53837629762408	36.9336649600951
5	14.396553744615225	36.73355739618343	33.381614658772904	15.488274200428437
6	35.46337589918446	34.84199759811224	16.163429618219848	13.531196884483448
7	30.864545911364775	30.10985652464131	20.320082050205126	18.705515513788782
8	28.63223223786287	32.97352027112542	19.011217205026885	19.383030285984823
9	27.220073781365283	13.88655294773898	18.36049743827725	40.53287583261849
10	15.840493500771094	26.548728982389036	31.548799294998897	26.06197822184097
11	38.19746593354052	20.816938776466838	21.594127490824206	19.391467799168435
12	24.5096632963489	23.5713180801845	28.669638546310228	23.24938007715637
13	29.819671593236862	19.04618600966564	25.235664430725674	25.898477966371825
14	23.38644279131686	19.300061406345947	24.862726348009918	32.45076945432727
15	25.43272723863631	27.11029235983181	22.125222070659486	25.33175833087239
16	26.050634454116334	25.60607125948634	23.816380963095256	24.526913323302068
17	24.310444235069117	25.81935284273882	25.217758152747116	24.652444769444955
18	25.29003951568674	24.511538299278595	26.32429113170489	23.87413105332977
19	25.54597741558971	25.081726690197954	25.15710180797158	24.21519408624076
20	25.587883731068327	24.026100040781316	25.36719588624357	25.018820341906782
21	26.52444769444952	24.32103800162188	24.31756924620195	24.836945057726652
22	25.579539968031202	24.467756980870284	24.922163940881155	25.030539110217358
23	24.158100247031637	24.230850360703688	25.563508692982335	26.04754069928234
24	24.628257231651922	25.054632897863904	25.238195684680758	25.078914185803413
25	24.981132783019973	24.321131751768363	25.15682055753212	25.54091490767954
26	24.425663165098698	25.18794560616501	25.630165047132884	24.75622618160341
27	25.453352270862922	24.71281986378104	24.96641401002189	24.86741385533415
28	24.543507099229842	24.498038278184808	25.69935265523852	25.259101967346826
29	24.441225689415138	24.91138267403543	25.61394627179105	25.033445364758382
30	24.5206320634876	24.951695237023806	25.873915427992856	24.653757271495735
31	25.090914204553442	24.777319964562444	24.682351066173542	25.44941476471057
32	24.84482007003136	24.80244500382032	24.872851363830257	25.47988356231807
33	24.352163050254767	24.456881963878068	25.45935228023794	25.731602705629225
34	25.071976674963555	24.773382458410094	25.63513380489657	24.519507061729783
35	25.67535261773847	24.552788363731818	25.42447722574567	24.347381792784052
36	24.40072562613379	25.323883318567685	25.198070621985348	25.077320433313176
37	25.718946435853805	24.903038910998298	24.902195159679938	24.47581949346796
38	24.751069923546755	24.656101025157852	25.333070832923177	25.259758218372212
39	25.45053976646838	24.406538135215836	24.9115701743284	25.231351923987383
40	25.602508753919928	24.638100997032808	25.277289495764837	24.482100753282428
41	24.37138183028411	24.974664022912535	25.84522788316857	24.808726263634785
42	25.902040471938236	25.13600802501254	25.200976876526372	23.760974626522856
43	25.03503911724862	23.967693699521405	25.35050836016931	25.64675882306066
44	24.488944513975802	24.830663797912184	25.32538332091144	25.355008367200572
45	24.670819798155936	25.061195408117825	25.110226734729274	25.157758058996965
46	24.383100598594684	24.494569522764877	25.589008732826144	25.53332114581429
47	24.865070101672035	24.37869434170991	25.92960301500471	24.826632541613346
48	25.367570886829512	25.385570914954553	24.58597591558737	24.660882282628567
49	24.377569339952093	25.07441417877215	25.34797710621423	25.20003937506152
50	24.21622533785209	25.71857143526787	24.801788752794927	25.263414474085117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	10.0
2	13.0
3	56.0
4	99.0
5	111.0
6	123.0
7	133.0
8	143.0
9	177.0
10	211.0
11	341.5
12	472.0
13	854.0
14	1236.0
15	1685.0
16	2134.0
17	2461.0
18	2788.0
19	2961.0
20	3134.0
21	3647.0
22	4160.0
23	4523.0
24	4886.0
25	5654.5
26	6423.0
27	8060.5
28	9698.0
29	11390.0
30	13082.0
31	15007.0
32	16932.0
33	19667.0
34	22402.0
35	25728.5
36	29055.0
37	34088.0
38	39121.0
39	42408.0
40	45695.0
41	49908.0
42	54121.0
43	55945.0
44	57769.0
45	64491.5
46	71214.0
47	76543.5
48	81873.0
49	83335.0
50	84797.0
51	80410.0
52	76023.0
53	74630.0
54	73237.0
55	73824.0
56	74411.0
57	72191.0
58	69971.0
59	64377.0
60	58783.0
61	51554.0
62	44325.0
63	38945.0
64	33565.0
65	28816.5
66	24068.0
67	21430.0
68	18792.0
69	17432.0
70	16072.0
71	12419.0
72	8766.0
73	7823.0
74	6880.0
75	5104.0
76	3328.0
77	2943.5
78	2559.0
79	2179.5
80	1800.0
81	1404.0
82	1008.0
83	861.5
84	715.0
85	597.0
86	479.0
87	350.0
88	221.0
89	138.0
90	55.0
91	32.5
92	10.0
93	8.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014812523144567413
2	0.0035625055664149476
3	1.8750029296920776E-4
4	3.750005859384155E-4
5	0.0
6	3.750005859384155E-4
7	9.375014648460388E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1066665.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.81039948486889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.44544947768293	47.31029020522371
2	12.215743276927887	14.36825484241461
3	3.3022248716689546	5.826154916751634
4	1.3734981945326172	3.2310391004883754
5	0.7036087778696375	2.0689756653786877
6	0.42550185350826414	1.5014360391823907
7	0.29177547851380764	1.2011602715900067
8	0.18850729683771109	0.8868951546270842
9	0.14612433368353767	0.773426739455022
>10	0.7607465910713818	8.098052348539635
>50	0.06805792742110976	2.810065250899795
>100	0.07123791682117127	8.521504062594582
>500	0.006083236840793685	2.4504511895940437
>1k	0.001440766620187978	0.9522942132604792
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1353	0.12684394819366906	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1263	0.1184064350100547	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1175	0.11015642211940956	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1111	0.10415641274439491	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1107	0.10378141215845652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.375014648460388E-5
2	0.0	0.0	0.0	0.0	9.375014648460388E-5
3	0.0	0.0	0.0	0.0	9.375014648460388E-5
4	0.0	0.0	0.0	0.0	9.375014648460388E-5
5	0.0	0.0	0.0	0.0	2.8125043945381164E-4
6	0.0	0.0	0.0	0.0	2.8125043945381164E-4
7	0.0	0.0	0.0	0.0	2.8125043945381164E-4
8	0.0	0.0	0.0	0.0	2.8125043945381164E-4
9	0.0	0.0	0.0	0.0	2.8125043945381164E-4
10	0.0	0.0	0.0	9.375014648460388E-5	2.8125043945381164E-4
11	0.0	0.0	0.0	9.375014648460388E-5	2.8125043945381164E-4
12	0.0	0.0	0.0	9.375014648460388E-5	2.8125043945381164E-4
13	0.0	0.0	0.0	9.375014648460388E-5	2.8125043945381164E-4
14	0.0	0.0	0.0	9.375014648460388E-5	2.8125043945381164E-4
15	0.0	0.0	0.0	9.375014648460388E-5	2.8125043945381164E-4
16	0.0	0.0	0.0	9.375014648460388E-5	2.8125043945381164E-4
17	0.0	0.0	0.0	1.8750029296920776E-4	2.8125043945381164E-4
18	0.0	0.0	0.0	3.750005859384155E-4	2.8125043945381164E-4
19	0.0	0.0	0.0	4.687507324230194E-4	2.8125043945381164E-4
20	0.0	0.0	0.0	9.375014648460388E-4	2.8125043945381164E-4
21	0.0	0.0	0.0	0.0010312516113306427	2.8125043945381164E-4
22	0.0	0.0	0.0	0.0012187519042998504	3.750005859384155E-4
23	0.0	0.0	0.0	0.002250003515630493	3.750005859384155E-4
24	0.0	0.0	0.0	0.003656255712899551	3.750005859384155E-4
25	0.0	0.0	0.0	0.004875007617199402	3.750005859384155E-4
26	0.0	0.0	0.0	0.006937510839860687	3.750005859384155E-4
27	0.0	0.0	0.0	0.009562514941429595	3.750005859384155E-4
28	0.0	0.0	0.0	0.014625022851598205	3.750005859384155E-4
29	0.0	0.0	0.0	0.021843784130912706	3.750005859384155E-4
30	0.0	0.0	0.0	0.039468811670018235	3.750005859384155E-4
31	0.0	0.0	0.0	0.07828137231464424	3.750005859384155E-4
32	0.0	0.0	0.0	0.1184064350100547	3.750005859384155E-4
33	0.0	0.0	0.0	0.16246900385781854	3.750005859384155E-4
34	0.0	0.0	0.0	0.21046907885793573	3.750005859384155E-4
35	0.0	0.0	0.0	0.2670941673346365	3.750005859384155E-4
36	0.0	0.0	0.0	0.3527818012215644	3.750005859384155E-4
37	0.0	0.0	0.0	0.4825320039562562	3.750005859384155E-4
38	0.0	0.0	0.0	0.6405947509292983	5.625008789076233E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGGG	85	2.3521898E-7	23.294044	2
GTATAGG	165	0.0	22.669783	1
CGCAATA	380	0.0	20.84204	36
ATTTCGT	160	1.8189894E-12	20.624935	42
TCTAGCG	395	0.0	20.050568	28
TAGCGGC	430	0.0	19.953424	30
GTCCTAC	320	0.0	19.94024	1
CTAGCGG	410	0.0	19.853596	29
CGGTCCA	420	0.0	19.380892	10
CGAGCCG	435	0.0	19.21833	15
GTAGGAC	340	0.0	18.764647	3
TAGGACG	225	0.0	18.577717	4
CGCCGGT	415	0.0	18.554157	7
TCCTACA	380	0.0	18.526257	2
CAATACG	430	0.0	18.418547	38
ACGCGCT	85	1.4306673E-4	18.11759	15
GCCGGTC	475	0.0	18.063099	8
TCTTGCG	440	0.0	17.999943	2
CGAATGC	430	0.0	17.90692	43
CTTACAC	160	1.1004886E-9	17.874945	3
>>END_MODULE