FastQCFastQC Report
Thu 2 Feb 2017
SRR4062519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831589
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37550.2050132425997317No Hit
GTACATGGAAGCAGTGGTATCAACG33730.18415703523006527No Hit
TCCATGTACTCTGCGTTGATACCAC30220.1649933473066283No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26120.1426084126952062No Hit
TATCAACGCAGAGTACTTTTTTTTT24160.1319073220029166No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20540.11214306266307561No Hit
GCGCAAGACGGACCAGAGCGAAAGC18450.10073220575139946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA2750.012.782866510
AATCGTA604.0888658E-412.66805313
GTCCTAT4550.012.101951
GTCTATA550.003083915412.0829811
CGTCTTA1750.011.9444915
ATCCCCG2550.011.92287312
AACGGAC1302.6102498E-911.69390715
GCTCGTA1302.6120688E-911.6932679
TCCAACG2300.011.56901418
GTCCTAA5450.011.4970441
CGCATCG3000.011.40124713
CCTATTC4000.011.3953273
TGTAGGA10750.011.3953262
ATAACGA1604.5474735E-1111.28248412
CGCCAGT2950.011.27488518
CTCGTAG1354.7220965E-911.26018210
ATCCCGT1354.7220965E-911.26018210
GTATTAA1354.778485E-911.2518761
CGAGCCG2800.011.1979615
AAGACGG3500.011.1267465
Produced by FastQC (version 0.11.5)