##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062519_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1831589
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.123481851004783	32.0	32.0	32.0	32.0	32.0
2	30.817729850965474	32.0	32.0	32.0	32.0	32.0
3	30.80026687209849	32.0	32.0	32.0	32.0	32.0
4	30.85826896754676	32.0	32.0	32.0	32.0	32.0
5	30.704879205979072	32.0	32.0	32.0	32.0	32.0
6	34.31177627731986	36.0	36.0	36.0	32.0	36.0
7	34.19949016946487	36.0	36.0	36.0	32.0	36.0
8	34.198360003254	36.0	36.0	36.0	32.0	36.0
9	34.3324119111875	36.0	36.0	36.0	32.0	36.0
10	34.0421835903142	36.0	36.0	36.0	32.0	36.0
11	34.313236211835736	36.0	36.0	36.0	32.0	36.0
12	34.11431167145031	36.0	36.0	36.0	32.0	36.0
13	34.22134168746373	36.0	36.0	36.0	32.0	36.0
14	34.14259421737082	36.0	36.0	36.0	32.0	36.0
15	34.08988097220501	36.0	36.0	36.0	32.0	36.0
16	34.10014309978931	36.0	36.0	36.0	32.0	36.0
17	34.02885472668814	36.0	36.0	36.0	32.0	36.0
18	34.039605501015785	36.0	36.0	36.0	32.0	36.0
19	34.01548436903694	36.0	36.0	36.0	32.0	36.0
20	34.00116674646987	36.0	36.0	36.0	32.0	36.0
21	33.984037357725995	36.0	36.0	36.0	32.0	36.0
22	33.94335246608273	36.0	36.0	36.0	32.0	36.0
23	33.90785159771106	36.0	36.0	36.0	32.0	36.0
24	33.8860328381531	36.0	36.0	36.0	32.0	36.0
25	33.42168576028792	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	76.0
5	160.0
6	325.0
7	93.0
8	265.0
9	357.0
10	201.0
11	69.0
12	137.0
13	90.0
14	290.0
15	371.0
16	560.0
17	803.0
18	1074.0
19	1643.0
20	2539.0
21	3660.0
22	5485.0
23	8131.0
24	11220.0
25	15779.0
26	21848.0
27	28297.0
28	38815.0
29	51680.0
30	66447.0
31	90888.0
32	129395.0
33	184929.0
34	410891.0
35	755066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.96644698405071	17.77578176616087	11.010904047656748	26.246867202131668
2	16.438060731538993	20.29695294004595	37.76540564859222	25.499580679822838
3	19.320962073226198	24.273015113816356	28.368776900720338	28.037245912237108
4	12.455305755592104	15.619723785747205	36.5104743432649	35.414496115395785
5	14.05446795867496	36.92393230173659	34.18212860223229	14.839471137356167
6	33.3613000136556	35.831052847193774	17.255933360644544	13.551713778506077
7	29.335042289564278	30.682265809347577	21.367307033545917	18.615384867542232
8	27.35063752724785	33.60951408254056	20.031574443389776	19.00827394682182
9	27.270233226145635	14.35655684846312	19.00993107488638	39.36327885050486
10	15.603412180518037	26.89715903065737	32.30305786560522	25.19637092321938
11	36.91221679486787	21.284989308063338	22.834718839236128	18.968075057832664
12	24.353770281551313	23.918604276107363	29.591069693803863	22.136555748537464
13	29.518468755568723	19.978856617474232	25.480235747621915	25.02243887933513
14	22.79808522257347	20.365419211512716	25.587817756133184	31.248677809780627
15	24.667168435154622	28.140740048394967	22.68704130953923	24.50505020691118
16	24.982673088737837	25.978724520695	24.997922957009582	24.04067943355758
17	23.28517763744818	26.212729809155633	26.27570320289678	24.226389350499414
18	24.084378995890603	25.026691660885525	27.696295093599847	23.19263424962402
19	25.019052023778677	25.483352850040617	25.76506039245529	23.732534733725416
20	25.117418626923634	24.960641801031187	25.986961503308276	23.93497806873691
21	25.826364956352887	24.722558735545718	24.76837071182408	24.682705596277312
22	25.053599109825548	25.616683662728867	25.252904139475245	24.076813087970343
23	23.6423457990218	25.21920928294864	26.06510179467862	25.073343123350945
24	24.160508041475186	25.6835171900268	25.92723801651266	24.22873675198536
25	24.21162601519346	25.34769145991052	26.036131484227056	24.404551040668967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	173.0
1	173.0
2	599.0
3	1025.0
4	1025.0
5	1025.0
6	2810.0
7	4595.0
8	4595.0
9	4595.0
10	4852.5
11	5110.0
12	5110.0
13	5110.0
14	5764.0
15	6418.0
16	6418.0
17	6418.0
18	10494.5
19	14571.0
20	14571.0
21	14571.0
22	23734.5
23	32898.0
24	32898.0
25	32898.0
26	48635.5
27	64373.0
28	64373.0
29	64373.0
30	80788.0
31	97203.0
32	97203.0
33	97203.0
34	114239.0
35	131275.0
36	131275.0
37	131275.0
38	144799.0
39	158323.0
40	158323.0
41	158323.0
42	176427.5
43	194532.0
44	194532.0
45	194532.0
46	218887.5
47	243243.0
48	243243.0
49	243243.0
50	236925.0
51	230607.0
52	230607.0
53	230607.0
54	210607.0
55	190607.0
56	190607.0
57	190607.0
58	173894.5
59	157182.0
60	157182.0
61	157182.0
62	139221.0
63	121260.0
64	121260.0
65	121260.0
66	101706.5
67	82153.0
68	82153.0
69	82153.0
70	63017.0
71	43881.0
72	43881.0
73	43881.0
74	34474.0
75	25067.0
76	25067.0
77	25067.0
78	20250.0
79	15433.0
80	15433.0
81	15433.0
82	10996.5
83	6560.0
84	6560.0
85	6560.0
86	4817.5
87	3075.0
88	3075.0
89	3075.0
90	2070.5
91	1066.0
92	1066.0
93	1066.0
94	626.5
95	187.0
96	187.0
97	187.0
98	479.5
99	772.0
100	772.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008680986837112475
2	0.0028936622790374914
3	0.004695376528249515
4	0.01403153218325727
5	0.03499693435590626
6	0.0458072198511784
7	0.06726399863724886
8	0.08888456962779313
9	0.09701958244999287
10	0.11700223139579896
11	0.11864015344053715
12	0.13114295838203877
13	0.1187493482435197
14	0.12218898453746992
15	0.11247064707202326
16	0.11274363407947961
17	0.12257116634790882
18	0.12917745192835292
19	0.13032399735966965
20	0.12213438713597866
21	0.12955963373879184
22	0.14926929567714153
23	0.13731246475055267
24	0.13359984144914605
25	0.12999641295072203
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1831589.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.75082352426929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92856191932755	41.1079581730654
2	13.737648313507602	14.493445236486307
3	4.075470346369742	6.449532510592287
4	1.5576557466167975	3.286704936053865
5	0.7320334502669094	1.9307683674445848
6	0.40482964356123047	1.2813058250934788
7	0.2707098543434145	0.9996117426925074
8	0.195344605629932	0.8243671054402021
9	0.13378630074019404	0.6351603786279714
>10	0.7580352817395953	7.532896909552559
>50	0.09436985801881036	3.515486245488359
>100	0.09671604261356763	10.73417935560295
>500	0.01079173619251535	3.827813354887545
>1k	0.004046901072193257	3.3807698589721076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3755	0.2050132425997317	No Hit
GTACATGGAAGCAGTGGTATCAACG	3373	0.18415703523006527	No Hit
TCCATGTACTCTGCGTTGATACCAC	3022	0.1649933473066283	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2612	0.1426084126952062	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2416	0.1319073220029166	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2054	0.11214306266307561	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1845	0.10073220575139946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.275844089476405E-4
2	0.0	0.0	0.0	0.0	3.275844089476405E-4
3	0.0	0.0	0.0	0.0	3.275844089476405E-4
4	0.0	0.0	0.0	5.459740149127343E-5	3.275844089476405E-4
5	0.0	0.0	0.0	5.459740149127343E-5	3.275844089476405E-4
6	0.0	0.0	0.0	5.459740149127343E-5	4.367792119301874E-4
7	0.0	0.0	0.0	5.459740149127343E-5	4.367792119301874E-4
8	0.0	0.0	0.0	5.459740149127343E-5	4.913766134214608E-4
9	0.0	0.0	0.0	5.459740149127343E-5	4.913766134214608E-4
10	5.459740149127343E-5	0.0	0.0	5.459740149127343E-5	4.913766134214608E-4
11	5.459740149127343E-5	0.0	0.0	5.459740149127343E-5	4.913766134214608E-4
12	5.459740149127343E-5	5.459740149127343E-5	0.0	5.459740149127343E-5	0.001146545431316742
13	5.459740149127343E-5	2.183896059650937E-4	0.0	5.459740149127343E-5	0.0012011428328080154
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCA	275	0.0	12.7828665	10
AATCGTA	60	4.0888658E-4	12.668053	13
GTCCTAT	455	0.0	12.10195	1
GTCTATA	55	0.0030839154	12.082981	1
CGTCTTA	175	0.0	11.94449	15
ATCCCCG	255	0.0	11.922873	12
AACGGAC	130	2.6102498E-9	11.693907	15
GCTCGTA	130	2.6120688E-9	11.693267	9
TCCAACG	230	0.0	11.569014	18
GTCCTAA	545	0.0	11.497044	1
CGCATCG	300	0.0	11.401247	13
CCTATTC	400	0.0	11.395327	3
TGTAGGA	1075	0.0	11.395326	2
ATAACGA	160	4.5474735E-11	11.282484	12
CGCCAGT	295	0.0	11.274885	18
CTCGTAG	135	4.7220965E-9	11.260182	10
ATCCCGT	135	4.7220965E-9	11.260182	10
GTATTAA	135	4.778485E-9	11.251876	1
CGAGCCG	280	0.0	11.19796	15
AAGACGG	350	0.0	11.126746	5
>>END_MODULE