FastQCFastQC Report
Thu 2 Feb 2017
SRR4062519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831589
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24990.13643890632669228No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC19410.10597355629456172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG655.934198E-727.076824
CGCAATA5500.019.99992636
ATACGAA5750.019.51297240
TAGCGGC5900.018.64430
TAGGACG11600.018.206834
CAATACG6150.017.88611238
AATACGA6200.017.74186939
TTAGGAC7850.017.6564673
CTAGCGG6300.017.46025329
TGTAGGA13500.017.274482
AGGACGT12150.017.0205125
ATTTCGT5100.016.82346742
GTCCTAC13100.016.6277081
CGGTCCA6750.016.6221610
ATAACGC5700.016.5968823
GACGTGA6800.016.4999397
ACCGTAT800.001988926416.4999398
GCAATAC6950.016.46037137
TCCTACA13500.016.4596462
TCTAGCG6700.016.41784928
Produced by FastQC (version 0.11.5)