##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1831589
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240880459535408	32.0	32.0	32.0	32.0	32.0
2	31.33425894127995	32.0	32.0	32.0	32.0	32.0
3	31.44671484705357	32.0	32.0	32.0	32.0	32.0
4	31.56007324787384	32.0	32.0	32.0	32.0	32.0
5	31.488039620242315	32.0	32.0	32.0	32.0	32.0
6	35.05665517755348	36.0	36.0	36.0	36.0	36.0
7	35.07523467328096	36.0	36.0	36.0	36.0	36.0
8	35.00667890012443	36.0	36.0	36.0	36.0	36.0
9	35.15421472830422	36.0	36.0	36.0	36.0	36.0
10	35.01420242204993	36.0	36.0	36.0	36.0	36.0
11	35.16468323406616	36.0	36.0	36.0	36.0	36.0
12	35.06026788761016	36.0	36.0	36.0	36.0	36.0
13	35.117357660479506	36.0	36.0	36.0	36.0	36.0
14	35.07748736206649	36.0	36.0	36.0	36.0	36.0
15	35.031614625333525	36.0	36.0	36.0	36.0	36.0
16	35.052165087254835	36.0	36.0	36.0	36.0	36.0
17	35.02721953451348	36.0	36.0	36.0	36.0	36.0
18	35.04551839959729	36.0	36.0	36.0	36.0	36.0
19	35.0210953439882	36.0	36.0	36.0	36.0	36.0
20	35.00381635836424	36.0	36.0	36.0	36.0	36.0
21	34.994663104004225	36.0	36.0	36.0	36.0	36.0
22	34.98741093116414	36.0	36.0	36.0	36.0	36.0
23	34.945712165775184	36.0	36.0	36.0	32.0	36.0
24	34.92808703262577	36.0	36.0	36.0	32.0	36.0
25	34.89891782490504	36.0	36.0	36.0	32.0	36.0
26	34.855087576961864	36.0	36.0	36.0	32.0	36.0
27	34.839690017793295	36.0	36.0	36.0	32.0	36.0
28	34.821127447260274	36.0	36.0	36.0	32.0	36.0
29	34.79639100256662	36.0	36.0	36.0	32.0	36.0
30	34.777700128140104	36.0	36.0	36.0	32.0	36.0
31	34.77946034836418	36.0	36.0	36.0	32.0	36.0
32	34.74345500000273	36.0	36.0	36.0	32.0	36.0
33	34.72446875363414	36.0	36.0	36.0	32.0	36.0
34	34.716273137696284	36.0	36.0	36.0	32.0	36.0
35	34.690542474321475	36.0	36.0	36.0	32.0	36.0
36	34.655822894765144	36.0	36.0	36.0	32.0	36.0
37	34.64104174025941	36.0	36.0	36.0	32.0	36.0
38	34.606295407976354	36.0	36.0	36.0	32.0	36.0
39	34.57517488912633	36.0	36.0	36.0	32.0	36.0
40	34.57010497442385	36.0	36.0	36.0	32.0	36.0
41	34.5397635604931	36.0	36.0	36.0	32.0	36.0
42	34.51961711934282	36.0	36.0	36.0	32.0	36.0
43	34.508140199575344	36.0	36.0	36.0	32.0	36.0
44	34.48126790453535	36.0	36.0	36.0	32.0	36.0
45	34.4210808210794	36.0	36.0	36.0	32.0	36.0
46	34.426535101488376	36.0	36.0	36.0	32.0	36.0
47	34.40243362457407	36.0	36.0	36.0	32.0	36.0
48	34.360383797893526	36.0	36.0	36.0	32.0	36.0
49	34.34448339665722	36.0	36.0	36.0	32.0	36.0
50	33.79936710692191	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	6.0
21	40.0
22	144.0
23	419.0
24	1074.0
25	2596.0
26	5702.0
27	10654.0
28	17971.0
29	28680.0
30	43150.0
31	63983.0
32	95833.0
33	157169.0
34	338108.0
35	1066059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73264239650964	18.2956102941578	11.483566588217306	26.488180721115256
2	15.970263097117218	20.80169082771826	37.34442643277829	25.883619642386225
3	18.761212984185256	24.638973302860588	28.714248119931295	27.88556559302286
4	12.366611432174865	16.200230947512672	36.65775817120144	34.77539944911101
5	14.04310683237342	37.17116667549325	33.81353567858291	14.972190813550421
6	33.50669941804249	36.13383439516875	17.094776796170734	13.264689390618031
7	29.58338360844054	30.672983950001885	21.175984350200835	18.56764809135674
8	27.605265155010212	33.86671354763541	19.667567341799934	18.86045395555444
9	27.555090143039735	14.112991506282249	18.81453754090028	39.51738080977774
10	15.61704072256385	27.084951918798378	32.148751712311004	25.14925564632677
11	37.317924490701785	21.209780141723936	22.630622918132833	18.84167244944144
12	24.481584023489987	23.98998902046256	29.376022677576685	22.15240427847077
13	29.786758928995532	19.89469253200363	25.30846166907532	25.010086869925512
14	23.040922390339755	20.38213813251772	25.295631279724873	31.28130819741765
15	24.870535911713816	28.20327049354413	22.374779494744725	24.551414099997324
16	25.208002450331378	25.993932044798264	24.684085785621118	24.113979719249244
17	23.57837921061985	26.296729233468863	25.83183235977067	24.293059196140618
18	24.376920804831215	25.096350764281727	27.25262053877808	23.274107892108983
19	25.401550238617943	25.510526651994525	25.41290649812813	23.6750166112594
20	25.380475641642313	25.026302298168424	25.648931064774906	23.944290995414363
21	26.236726689229954	24.71678962911439	24.384182259229554	24.6623014224261
22	25.309389824900673	25.742620205733928	24.945989520574756	24.00200044879064
23	23.950023722570947	25.224108683771306	25.77199360773623	25.053873985921516
24	24.476015088537874	25.765714906564735	25.528270807479185	24.2299991974182
25	24.546554931264602	25.379656680619945	25.631514493699186	24.44227389441627
26	23.58443952218538	26.125293392786265	26.232195104906175	24.05807198012218
27	24.76128651132978	25.866829294126575	25.219795489053492	24.15208870549015
28	23.766849440567725	25.649149454380872	25.819711736639604	24.7642893684118
29	23.996158526831074	25.68878716786353	25.598210078789513	24.71684422651588
30	24.068500083807013	25.679451012208528	26.023087057194598	24.228961846789865
31	24.534707295140993	25.539900053996828	25.125997153291486	24.79939549757069
32	24.096836135180983	25.95309318848279	25.298524942003908	24.65154573433232
33	23.772254583315362	25.4199495629205	25.91127157894047	24.896524274823665
34	24.261228911071207	25.86186093059087	26.180764352701395	23.696145805636526
35	25.009868480319547	25.61502607844882	25.693646336596252	23.681459104635376
36	23.789125180376164	26.025052563648288	25.751847166585957	24.433975089389595
37	24.897780015057965	25.861642540984903	24.906406404493584	24.33417103946355
38	24.087445382124486	25.826863996234962	25.501463483346974	24.584227138293578
39	24.642318773480294	25.366826291269497	25.199103073888303	24.79175186136191
40	25.26582109851064	25.392104888159956	25.597500312570126	23.744573700759286
41	24.053322006192438	25.551310910908505	26.35542144007198	24.039945642827078
42	25.070144011565915	26.204241235342646	25.474383172207304	23.25123158088414
43	24.14095083558593	25.344714343665526	25.838547840154096	24.675786980594445
44	23.776731570237647	25.832542125990056	25.723074336000053	24.667651967772247
45	24.05637946067595	26.087511990954305	25.519098444028653	24.337010104341093
46	23.975123199238254	25.25701768849511	25.80381156003595	24.96404755223069
47	24.545517580636268	25.002825415527173	26.011184823669502	24.44047218016706
48	24.432227972541874	26.112790587844763	25.15498837348335	24.29999306613001
49	23.96290870932289	25.559773508139656	25.877366592614393	24.59995118992307
50	23.566460979719217	26.571943659757174	25.142035372622416	24.719559987901196
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	10.5
2	14.0
3	48.0
4	82.0
5	116.5
6	151.0
7	138.0
8	125.0
9	219.0
10	313.0
11	456.5
12	600.0
13	1056.0
14	1512.0
15	2161.0
16	2810.0
17	3647.0
18	4484.0
19	5242.0
20	6000.0
21	7395.0
22	8790.0
23	9991.0
24	11192.0
25	13603.5
26	16015.0
27	20339.5
28	24664.0
29	28517.0
30	32370.0
31	36036.5
32	39703.0
33	46735.0
34	53767.0
35	61735.0
36	69703.0
37	78307.5
38	86912.0
39	88564.0
40	90216.0
41	94500.5
42	98785.0
43	99045.5
44	99306.0
45	108121.5
46	116937.0
47	124351.5
48	131766.0
49	132857.0
50	133948.0
51	126741.5
52	119535.0
53	116065.0
54	112595.0
55	112955.5
56	113316.0
57	111239.5
58	109163.0
59	99195.0
60	89227.0
61	78692.0
62	68157.0
63	60217.0
64	52277.0
65	44727.0
66	37177.0
67	33660.5
68	30144.0
69	27771.0
70	25398.0
71	20207.0
72	15016.0
73	13202.0
74	11388.0
75	8530.5
76	5673.0
77	5038.5
78	4404.0
79	3801.0
80	3198.0
81	2518.0
82	1838.0
83	1584.0
84	1330.0
85	1098.5
86	867.0
87	678.0
88	489.0
89	320.0
90	151.0
91	96.0
92	41.0
93	27.5
94	14.0
95	12.0
96	10.0
97	8.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014140726986239817
2	0.003275844089476406
3	3.8218181043891394E-4
4	2.1838960596509368E-4
5	0.0
6	6.55168817895281E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.6379220447382026E-4
47	0.0
48	0.0
49	0.0
50	1.6379220447382026E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1831589.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.064489514900515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3878981265649	43.94777492611888
2	13.66652620839578	15.324136306314365
3	3.9118095058097406	6.579408090682751
4	1.5168741172304898	3.401710921635711
5	0.7095527014288121	1.989035499476249
6	0.40962237000688045	1.377916144099167
7	0.2537275493497429	0.9957573871112047
8	0.1826841188275128	0.8193673491635144
9	0.12620543126903797	0.6368078770295829
>10	0.6601780566093252	7.005827530548394
>50	0.0843174564677483	3.3158083919388037
>100	0.08013567512184475	9.607689688434887
>500	0.007827052649140416	2.967518296856827
>1k	0.0026416302690848905	2.0312415905897443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2499	0.13643890632669228	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1941	0.10597355629456172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.275844089476405E-4
2	5.459740149127343E-5	5.459740149127343E-5	0.0	0.0	3.275844089476405E-4
3	5.459740149127343E-5	5.459740149127343E-5	0.0	0.0	3.275844089476405E-4
4	5.459740149127343E-5	5.459740149127343E-5	0.0	0.0	3.82181810438914E-4
5	5.459740149127343E-5	5.459740149127343E-5	0.0	0.0	3.82181810438914E-4
6	5.459740149127343E-5	5.459740149127343E-5	0.0	0.0	7.097662193865545E-4
7	5.459740149127343E-5	5.459740149127343E-5	0.0	0.0	7.097662193865545E-4
8	5.459740149127343E-5	5.459740149127343E-5	0.0	0.0	7.097662193865545E-4
9	5.459740149127343E-5	5.459740149127343E-5	0.0	0.0	7.097662193865545E-4
10	5.459740149127343E-5	5.459740149127343E-5	0.0	0.0	8.189610223691014E-4
11	5.459740149127343E-5	5.459740149127343E-5	0.0	5.459740149127343E-5	8.189610223691014E-4
12	5.459740149127343E-5	5.459740149127343E-5	0.0	5.459740149127343E-5	0.001037350628334195
13	5.459740149127343E-5	1.6379220447382026E-4	0.0	5.459740149127343E-5	0.001146545431316742
14	5.459740149127343E-5	1.6379220447382026E-4	0.0	5.459740149127343E-5	0.0012011428328080154
15	5.459740149127343E-5	1.6379220447382026E-4	0.0	5.459740149127343E-5	0.001419532438773109
16	5.459740149127343E-5	2.183896059650937E-4	0.0	1.0919480298254685E-4	0.0016379220447382027
17	5.459740149127343E-5	2.729870074563671E-4	0.0	1.0919480298254685E-4	0.0016379220447382027
18	5.459740149127343E-5	2.729870074563671E-4	0.0	1.0919480298254685E-4	0.001692519446229476
19	5.459740149127343E-5	2.729870074563671E-4	0.0	1.0919480298254685E-4	0.001692519446229476
20	5.459740149127343E-5	2.729870074563671E-4	0.0	1.6379220447382026E-4	0.00207470125666839
21	5.459740149127343E-5	2.729870074563671E-4	0.0	2.729870074563671E-4	0.00207470125666839
22	5.459740149127343E-5	2.729870074563671E-4	0.0	3.82181810438914E-4	0.00207470125666839
23	5.459740149127343E-5	2.729870074563671E-4	0.0	7.097662193865545E-4	0.0021292986581596634
24	5.459740149127343E-5	2.729870074563671E-4	0.0	0.0010919480298254684	0.002183896059650937
25	5.459740149127343E-5	2.729870074563671E-4	0.0	0.001419532438773109	0.002183896059650937
26	5.459740149127343E-5	2.729870074563671E-4	0.0	0.0017471168477207497	0.0024022856656160307
27	5.459740149127343E-5	2.729870074563671E-4	0.0	0.003002857082020038	0.002456883067107304
28	5.459740149127343E-5	2.729870074563671E-4	0.0	0.0057327271565837095	0.002456883067107304
29	5.459740149127343E-5	2.729870074563671E-4	0.0	0.010591895889307045	0.0025114804685985774
30	5.459740149127343E-5	2.729870074563671E-4	0.0	0.020801609968175174	0.0025114804685985774
31	5.459740149127343E-5	2.729870074563671E-4	0.0	0.047390544494425334	0.0025114804685985774
32	5.459740149127343E-5	2.729870074563671E-4	0.0	0.07883864775339883	0.002566077870089851
33	5.459740149127343E-5	2.729870074563671E-4	0.0	0.11159708864816288	0.002566077870089851
34	5.459740149127343E-5	2.729870074563671E-4	0.0	0.1522721527591616	0.0027298700745636713
35	5.459740149127343E-5	3.275844089476405E-4	0.0	0.19665984017156687	0.0027298700745636713
36	5.459740149127343E-5	3.275844089476405E-4	0.0	0.26441521542223717	0.0027298700745636713
37	5.459740149127343E-5	3.275844089476405E-4	0.0	0.3654750055825843	0.0027298700745636713
38	5.459740149127343E-5	3.275844089476405E-4	0.0	0.504589184582349	0.002839064877546218
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACACG	65	5.934198E-7	27.07682	4
CGCAATA	550	0.0	19.999926	36
ATACGAA	575	0.0	19.512972	40
TAGCGGC	590	0.0	18.644	30
TAGGACG	1160	0.0	18.20683	4
CAATACG	615	0.0	17.886112	38
AATACGA	620	0.0	17.741869	39
TTAGGAC	785	0.0	17.656467	3
CTAGCGG	630	0.0	17.460253	29
TGTAGGA	1350	0.0	17.27448	2
AGGACGT	1215	0.0	17.020512	5
ATTTCGT	510	0.0	16.823467	42
GTCCTAC	1310	0.0	16.627708	1
CGGTCCA	675	0.0	16.62216	10
ATAACGC	570	0.0	16.596882	3
GACGTGA	680	0.0	16.499939	7
ACCGTAT	80	0.0019889264	16.499939	8
GCAATAC	695	0.0	16.460371	37
TCCTACA	1350	0.0	16.459646	2
TCTAGCG	670	0.0	16.417849	28
>>END_MODULE