FastQCFastQC Report
Thu 2 Feb 2017
SRR4062518_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062518_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434314
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT48400.33744354444005986No Hit
GTATCAACGCAGAGTACTTTTTTTT47100.32837997816377723No Hit
GTCCTACAGTGGACATTTCTAAATT42580.2968666554185485No Hit
CTGTAGGACGTGGAATATGGCAAGA40230.280482516380653No Hit
CTTTAGGACGTGAAATATGGCGAGG38810.27058231321732895No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31230.21773474985254276No Hit
TATCAACGCAGAGTACTTTTTTTTT29790.2076951072080451No Hit
GTCCTACAGTGTGCATTTCTCATTT26240.18294459929973494No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23480.1637019508977811No Hit
CTGAAGGACCTGGAATATGGCGAGA20790.1449473406799348No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18830.1312822715249241No Hit
GTCCTTCAGTGTGCATTTCTCATTT18740.13065479385964301No Hit
CTGTAGGACCTGGAATATGGCGAGA17680.12326450135744335No Hit
ATTTAGAAATGTCCACTGTAGGACG16790.11705944444521911No Hit
GTACATGGAAGCAGTGGTATCAACG16700.116431966779938No Hit
GGTATCAACGCAGAGTACTTTTTTT15180.10583456621074605No Hit
TTTCTAAATTTTCCACCTTTTTCAG14830.10339437529020842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG400.005275813414.2512313
TTGGCGA500.001496108713.30393517
TAGACAA604.097136E-412.6646665
CAAGACG1950.012.6637824
CTAATGT604.0995487E-412.6637824
TAGGACC10500.012.4828714
ACGGTAT2150.012.3727299
GTTCTAG853.965113E-612.2878881
TACGCTA2250.012.2450749
GGACCTA1103.8135113E-812.0906886
CTACGCA550.003073482812.0881574
AAGACGG2050.012.0468785
GCGTTAT951.0459571E-611.9939191
ACGCTAT2300.011.97887710
ATACGCT2300.011.9788778
TAGAAAT6900.011.9755334
CGCAAGA2150.011.9254352
CGAACGA802.8583518E-511.878098516
AAATGTC6900.011.8403637
CGTCGTA1850.011.81141910
Produced by FastQC (version 0.11.5)