##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062518_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1434314
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09947612586923	32.0	32.0	32.0	32.0	32.0
2	30.8243432051838	32.0	32.0	32.0	32.0	32.0
3	30.80022575252002	32.0	32.0	32.0	32.0	32.0
4	30.848446713899467	32.0	32.0	32.0	32.0	32.0
5	30.707765524146037	32.0	32.0	32.0	32.0	32.0
6	34.32921870664303	36.0	36.0	36.0	32.0	36.0
7	34.1950479462656	36.0	36.0	36.0	32.0	36.0
8	34.190023941758916	36.0	36.0	36.0	32.0	36.0
9	34.31276136187753	36.0	36.0	36.0	32.0	36.0
10	34.02745005626383	36.0	36.0	36.0	32.0	36.0
11	34.33075114654113	36.0	36.0	36.0	32.0	36.0
12	34.110113266690554	36.0	36.0	36.0	32.0	36.0
13	34.23459507471865	36.0	36.0	36.0	32.0	36.0
14	34.14196682176985	36.0	36.0	36.0	32.0	36.0
15	34.10259329547087	36.0	36.0	36.0	32.0	36.0
16	34.11650935569199	36.0	36.0	36.0	32.0	36.0
17	34.04770085211467	36.0	36.0	36.0	32.0	36.0
18	34.083270469367235	36.0	36.0	36.0	32.0	36.0
19	34.033286992945754	36.0	36.0	36.0	32.0	36.0
20	34.00879514527502	36.0	36.0	36.0	32.0	36.0
21	33.97200822135181	36.0	36.0	36.0	32.0	36.0
22	33.937837182095414	36.0	36.0	36.0	32.0	36.0
23	33.9344376475444	36.0	36.0	36.0	32.0	36.0
24	33.909365731631986	36.0	36.0	36.0	32.0	36.0
25	33.49302453995429	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	52.0
5	137.0
6	217.0
7	56.0
8	213.0
9	248.0
10	158.0
11	53.0
12	104.0
13	86.0
14	248.0
15	317.0
16	527.0
17	657.0
18	879.0
19	1292.0
20	2106.0
21	3030.0
22	4522.0
23	6662.0
24	9042.0
25	12601.0
26	17122.0
27	22022.0
28	29895.0
29	39404.0
30	50970.0
31	69439.0
32	99626.0
33	142878.0
34	320048.0
35	599695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94401806750492	18.548965713437042	11.697789045564111	25.809227173493927
2	15.152841990184479	20.730774567215544	40.4466696740268	23.66971376857318
3	19.24814902872378	25.12314824434006	28.6778358260467	26.95086690088946
4	12.274303788112276	16.32724782758462	37.06766109901696	34.33078728528615
5	13.38585422940603	37.70652554465555	34.31723997483684	14.590380251101587
6	32.00838118780459	36.44226389721048	18.057986157369076	13.491368757615854
7	28.5320522971935	31.3119016508037	21.736989011617645	18.419057040385148
8	25.982198181300554	35.93354880846717	20.2315659906553	17.852687019576976
9	27.492130042198742	14.73780880555988	19.950090057020994	37.81997109522038
10	16.172994961840374	27.310063894864538	32.59957310357054	23.91736803972454
11	35.50117266026357	21.099997207951755	24.921822649095375	18.477007482689302
12	25.760305706943814	23.85759551771295	30.1977754709829	20.18432330436033
13	29.822437956591603	21.05490197720829	25.700217642287658	23.42244242391245
14	22.03733525433359	22.273202255779232	25.983971654077553	29.705490835809623
15	23.038884499523995	31.63720828947846	22.800323293010113	22.52358391798743
16	22.639133754618715	26.69543635422032	28.158698119291603	22.506731771869358
17	21.78398964678158	27.981262014884845	27.600345665706637	22.634402672626933
18	22.370160739740676	25.345863391464725	31.113254990219907	21.170720878574688
19	24.869125115621564	25.170002966892962	26.662594460636313	23.29827745684916
20	25.017642636616134	25.818070387663344	27.21328708670684	21.95099988901368
21	24.687172993247476	25.150977206996718	25.42616026097015	24.735689538785664
22	24.818042681070573	27.66728762103723	25.91558185845033	21.599087839441864
23	21.99069080327602	27.30497567977592	27.751864597056443	22.95246891989162
24	24.139974825977415	26.08638892398855	27.808917579859106	21.964718670174925
25	22.782522376869625	26.81908487643333	27.991086948326917	22.40730579837013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	377.0
1	377.0
2	819.0
3	1261.0
4	1261.0
5	1261.0
6	3024.0
7	4787.0
8	4787.0
9	4787.0
10	4963.0
11	5139.0
12	5139.0
13	5139.0
14	5177.5
15	5216.0
16	5216.0
17	5216.0
18	8987.0
19	12758.0
20	12758.0
21	12758.0
22	22085.5
23	31413.0
24	31413.0
25	31413.0
26	49079.5
27	66746.0
28	66746.0
29	66746.0
30	86882.5
31	107019.0
32	107019.0
33	107019.0
34	118354.5
35	129690.0
36	129690.0
37	129690.0
38	134889.0
39	140088.0
40	140088.0
41	140088.0
42	149458.5
43	158829.0
44	158829.0
45	158829.0
46	172032.5
47	185236.0
48	185236.0
49	185236.0
50	176478.0
51	167720.0
52	167720.0
53	167720.0
54	145613.5
55	123507.0
56	123507.0
57	123507.0
58	112002.5
59	100498.0
60	100498.0
61	100498.0
62	89078.0
63	77658.0
64	77658.0
65	77658.0
66	64682.0
67	51706.0
68	51706.0
69	51706.0
70	39840.5
71	27975.0
72	27975.0
73	27975.0
74	22413.5
75	16852.0
76	16852.0
77	16852.0
78	13941.5
79	11031.0
80	11031.0
81	11031.0
82	7942.5
83	4854.0
84	4854.0
85	4854.0
86	3602.5
87	2351.0
88	2351.0
89	2351.0
90	1595.0
91	839.0
92	839.0
93	839.0
94	515.5
95	192.0
96	192.0
97	192.0
98	382.0
99	572.0
100	572.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009551604460390124
2	0.003834585732273407
3	0.004671222619314878
4	0.013665069155010688
5	0.03290771755696451
6	0.043714277347916844
7	0.0621202888628292
8	0.08380312818532065
9	0.09293641420219004
10	0.11489813248702864
11	0.11671084574228516
12	0.12765684501441107
13	0.1176869221105002
14	0.120405991993385
15	0.10862335583421762
16	0.10848391635304402
17	0.11963907484693032
18	0.12633216994326207
19	0.1282146029391054
20	0.11887215770047563
21	0.12640188968384886
22	0.14710865263812528
23	0.13713872973421443
24	0.1314217110060977
25	0.12605329098091492
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1434314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.22842972362783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.2162170415912	43.762401309408695
2	11.670386646854169	12.423927109592835
3	2.850274330642011	4.551468807049159
4	1.0587346658380263	2.2541913502611193
5	0.5111584343980825	1.3604080401498984
6	0.30166436336662067	0.9634272219349858
7	0.20565940655316886	0.7662849088102848
8	0.1386043803365673	0.5902154814505565
9	0.10636554707606798	0.5095503942798341
>10	0.7014749585083767	7.5067283313890005
>50	0.10976915002370387	4.094771999912812
>100	0.11434847463112033	12.605512121552076
>500	0.010752933460139863	3.9007850153152166
>1k	0.004589666720791405	4.710327908893563
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	4840	0.33744354444005986	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4710	0.32837997816377723	No Hit
GTCCTACAGTGGACATTTCTAAATT	4258	0.2968666554185485	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4023	0.280482516380653	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3881	0.27058231321732895	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3123	0.21773474985254276	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2979	0.2076951072080451	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2624	0.18294459929973494	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2348	0.1637019508977811	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2079	0.1449473406799348	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1883	0.1312822715249241	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1874	0.13065479385964301	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1768	0.12326450135744335	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1679	0.11705944444521911	No Hit
GTACATGGAAGCAGTGGTATCAACG	1670	0.116431966779938	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1518	0.10583456621074605	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1483	0.10339437529020842	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.971974058678923E-5	0.0
8	0.0	0.0	0.0	1.3943948117357846E-4	0.0
9	0.0	0.0	0.0	2.0915922176036769E-4	0.0
10	0.0	0.0	0.0	2.0915922176036769E-4	0.0
11	0.0	0.0	0.0	2.0915922176036769E-4	0.0
12	0.0	0.0	0.0	2.0915922176036769E-4	0.0
13	0.0	0.0	0.0	2.788789623471569E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	40	0.0052758134	14.25123	13
TTGGCGA	50	0.0014961087	13.303935	17
TAGACAA	60	4.097136E-4	12.664666	5
CAAGACG	195	0.0	12.663782	4
CTAATGT	60	4.0995487E-4	12.663782	4
TAGGACC	1050	0.0	12.482871	4
ACGGTAT	215	0.0	12.372729	9
GTTCTAG	85	3.965113E-6	12.287888	1
TACGCTA	225	0.0	12.245074	9
GGACCTA	110	3.8135113E-8	12.090688	6
CTACGCA	55	0.0030734828	12.088157	4
AAGACGG	205	0.0	12.046878	5
GCGTTAT	95	1.0459571E-6	11.993919	1
ACGCTAT	230	0.0	11.978877	10
ATACGCT	230	0.0	11.978877	8
TAGAAAT	690	0.0	11.975533	4
CGCAAGA	215	0.0	11.925435	2
CGAACGA	80	2.8583518E-5	11.8780985	16
AAATGTC	690	0.0	11.840363	7
CGTCGTA	185	0.0	11.811419	10
>>END_MODULE