FastQCFastQC Report
Thu 2 Feb 2017
SRR4062518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434314
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22630.157775772947904No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA18430.12849348190145254No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC17590.12263702369216224No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA17240.12019683277162461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA30100.020.685632
CTGTAGG29900.020.6089951
TAGATCG654.9330964E-420.3074995
TAGGACC14300.020.1536564
GTAGGAC31050.019.4845183
GTCCTAC28250.019.3977281
GCATTCG5400.019.14796822
TCCTACA30050.018.8896562
GACGGCC5400.018.74056411
CGTATTG5650.018.69008627
GTCCTAT4950.018.6729981
CCTACAG29050.018.554043
TAGGACG30700.018.201784
TTAGGAC17750.018.0956043
GACGTGA16250.018.0059817
CCGCTAG5750.017.98243736
AGGACGT30850.017.9706555
CGGGGGC5950.017.74773217
AGGACCT21400.017.6820745
GTCCTAA16700.017.6586821
Produced by FastQC (version 0.11.5)