FastQCFastQC Report
Thu 2 Feb 2017
SRR4062517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888620
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC26370.13962575849032627No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25820.13671357922716057No Hit
GTATCAACGCAGAGTACTTTTTTTT24040.12728870815727886No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG23770.12585909288263386No Hit
GAATAGGACCGCGGTTCTATTTTGT23650.125223708316125No Hit
GAACTACGACGGTATCTGATCGTCT22130.11717550380701253No Hit
GGGTAGGCACACGCTGAGCCAGTCA21810.11548114496298884No Hit
GTATCTGATCGTCTTCGAACCTCCG21330.11293960669695334No Hit
GAATAACGCCGCCGCATCGCCAGTC20750.10986858129216043No Hit
GTCCTATTCCATTATTCCTAGCTGC20350.10775063273713083No Hit
GATTAAGAGGGACGGCCGGGGGCAT19350.1024557613495568No Hit
CTATTGGAGCTGGAATTACCGCGGC19170.1015026844997935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA807.796189E-915.4258341
GTGTAAT653.3996166E-614.6043391
AACCGCG2650.013.982427
CGAACGT1500.013.9298154
GAACCGT852.6978887E-713.4126446
GAACCGC3000.013.3008726
CGGTCCA4650.013.07577910
AAGACGG4500.012.8755495
GGTCCAA5300.012.72657611
CAAGACG4700.012.528754
ACGAACG1850.012.32709415
AGAACCG3350.012.1919215
TAGTACT550.003074672312.0878564
CGCATCG3550.012.0443213
AATTCCG1900.011.9979255
TAGACTG802.8722015E-511.8729465
CGCAAGA4650.011.8420742
CGCGGTC2900.011.79356510
GTCGGCG2750.011.74715912
ATCGCCA3750.011.6565716