##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062517_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1888620
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20817263398672	32.0	32.0	32.0	32.0	32.0
2	30.820493270218467	32.0	32.0	32.0	32.0	32.0
3	30.80541612394235	32.0	32.0	32.0	32.0	32.0
4	30.883694973049106	32.0	32.0	32.0	32.0	32.0
5	30.65654552000932	32.0	32.0	32.0	32.0	32.0
6	34.32253920852263	36.0	36.0	36.0	32.0	36.0
7	34.217306287130285	36.0	36.0	36.0	32.0	36.0
8	34.203599453569275	36.0	36.0	36.0	32.0	36.0
9	34.391500142961526	36.0	36.0	36.0	32.0	36.0
10	34.02435429043429	36.0	36.0	36.0	32.0	36.0
11	34.35252830108757	36.0	36.0	36.0	32.0	36.0
12	34.11398004892461	36.0	36.0	36.0	32.0	36.0
13	34.26075917865955	36.0	36.0	36.0	32.0	36.0
14	34.15655558026496	36.0	36.0	36.0	32.0	36.0
15	34.079263165697704	36.0	36.0	36.0	32.0	36.0
16	34.07749732608995	36.0	36.0	36.0	32.0	36.0
17	34.00144867681164	36.0	36.0	36.0	32.0	36.0
18	34.01491777064735	36.0	36.0	36.0	32.0	36.0
19	34.006776905888955	36.0	36.0	36.0	32.0	36.0
20	34.001634526797346	36.0	36.0	36.0	32.0	36.0
21	33.98784244580699	36.0	36.0	36.0	32.0	36.0
22	33.93775772786479	36.0	36.0	36.0	32.0	36.0
23	33.90071110122735	36.0	36.0	36.0	32.0	36.0
24	33.87152259321621	36.0	36.0	36.0	32.0	36.0
25	33.40723491226398	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	75.0
5	175.0
6	293.0
7	78.0
8	264.0
9	346.0
10	218.0
11	68.0
12	142.0
13	94.0
14	248.0
15	344.0
16	567.0
17	707.0
18	956.0
19	1361.0
20	2126.0
21	3152.0
22	5008.0
23	7473.0
24	10955.0
25	15821.0
26	22489.0
27	29457.0
28	40533.0
29	54262.0
30	70994.0
31	95981.0
32	136499.0
33	196313.0
34	428638.0
35	762974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79755239589528	17.12094358416042	10.781489034180778	26.300014985763525
2	17.490490068581643	19.27709695046787	35.944946180989454	27.28746679996103
3	19.24925735889819	22.73614928012793	28.14628308790435	29.868310273069532
4	12.81551721946611	14.396916213708636	35.03353231006477	37.75403425676048
5	15.184700042850643	35.76672724373965	33.3973356328256	15.651237080584105
6	35.93324236247367	34.309327762074474	16.037110177859333	13.72031969759252
7	31.305992113089165	29.730676247237646	20.3591548512237	18.604176788449486
8	28.912852700459773	32.24362113224505	19.070360972797204	19.77316519449797
9	27.21459817496314	13.937290724407564	18.348467776726064	40.499643323903236
10	16.01677193298648	26.164268467546787	31.224260993328862	26.594698606137868
11	38.41203957708812	20.656313860197358	21.527582304281335	19.40406425843319
12	24.678122851170155	23.302023485301937	28.15961819551575	23.86023546801216
13	29.500666608355004	18.56516196065023	25.26745175372333	26.666719677271438
14	23.534822176902267	19.24328634112284	24.419921954726107	32.80196952724879
15	25.49498232736047	26.77977367995878	21.708457635492003	26.01678635718875
16	26.52246116888507	25.2966366951187	22.976284436227285	25.204617699768946
17	24.67195455287639	25.580017006622402	24.59132153331029	25.156706907190916
18	25.741953817019713	24.205030547609102	25.382666856109203	24.67034877926198
19	25.89740476696662	24.78410169924647	24.650392935172455	24.668100598614455
20	25.881439905173977	24.026480039779432	24.284007332477383	25.808072722569207
21	26.914977765242526	23.961158624901657	24.11447984239512	25.0093837674607
22	26.116341762942945	23.777766701380568	24.46911063836105	25.636780897315436
23	24.641608512109332	23.631251504454244	25.152301138984534	26.574838844451893
24	25.08844675672251	24.61664235805707	24.62157300296952	25.673337882250895
25	25.284806032883118	24.116687767337012	24.622626704753905	25.975879495025968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	143.0
2	389.5
3	636.0
4	636.0
5	636.0
6	1724.5
7	2813.0
8	2813.0
9	2813.0
10	3125.0
11	3437.0
12	3437.0
13	3437.0
14	4092.5
15	4748.0
16	4748.0
17	4748.0
18	7415.5
19	10083.0
20	10083.0
21	10083.0
22	16305.5
23	22528.0
24	22528.0
25	22528.0
26	33394.0
27	44260.0
28	44260.0
29	44260.0
30	54928.5
31	65597.0
32	65597.0
33	65597.0
34	85762.0
35	105927.0
36	105927.0
37	105927.0
38	123290.5
39	140654.0
40	140654.0
41	140654.0
42	164075.5
43	187497.0
44	187497.0
45	187497.0
46	215958.5
47	244420.0
48	244420.0
49	244420.0
50	251942.0
51	259464.0
52	259464.0
53	259464.0
54	245785.5
55	232107.0
56	232107.0
57	232107.0
58	216071.5
59	200036.0
60	200036.0
61	200036.0
62	177044.0
63	154052.0
64	154052.0
65	154052.0
66	127677.5
67	101303.0
68	101303.0
69	101303.0
70	75956.0
71	50609.0
72	50609.0
73	50609.0
74	39109.0
75	27609.0
76	27609.0
77	27609.0
78	22850.5
79	18092.0
80	18092.0
81	18092.0
82	12531.0
83	6970.0
84	6970.0
85	6970.0
86	5154.5
87	3339.0
88	3339.0
89	3339.0
90	2313.5
91	1288.0
92	1288.0
93	1288.0
94	757.5
95	227.0
96	227.0
97	227.0
98	504.0
99	781.0
100	781.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008524742933994134
2	0.003600512543550317
3	0.004765384248816596
4	0.015461024451716068
5	0.03531679215511855
6	0.044265124800118606
7	0.06295602079825481
8	0.08514153191218984
9	0.0928720441380479
10	0.11304550412470482
11	0.11516345267973442
12	0.12808293886541494
13	0.1167519140960066
14	0.12067011892281136
15	0.11055691457254505
16	0.10870370958689414
17	0.12157024705869894
18	0.12622973387976408
19	0.12871832343192385
20	0.11744024737639122
21	0.12622973387976408
22	0.14444409145301862
23	0.13523101523863984
24	0.1300949899926931
25	0.12834768243479366
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1888620.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.15376648089493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.92926979857903	38.796708961869065
2	12.007893686483566	11.804664043856516
3	3.553547835947916	5.2401078152062
4	1.6330290714546618	3.210781185391805
5	0.9376965976379462	2.304565979511265
6	0.5703153614624928	1.6819888858676733
7	0.4139688509075889	1.4243689759513225
8	0.2961773191500252	1.1646584625950263
9	0.2282410878681323	1.0097018220974037
>10	1.2233055897139131	10.951398877446374
>50	0.09703628027855951	3.332540401666515
>100	0.08901831427686129	9.514101768664714
>500	0.014350144367544166	4.923544313282665
>1k	0.006150061871804643	4.6408685065934865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	2637	0.13962575849032627	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2582	0.13671357922716057	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2404	0.12728870815727886	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2377	0.12585909288263386	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2365	0.125223708316125	No Hit
GAACTACGACGGTATCTGATCGTCT	2213	0.11717550380701253	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2181	0.11548114496298884	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2133	0.11293960669695334	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2075	0.10986858129216043	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2035	0.10775063273713083	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1935	0.1024557613495568	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1917	0.1015026844997935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.294871387573996E-5
6	0.0	0.0	0.0	0.0	1.0589742775147991E-4
7	0.0	0.0	0.0	0.0	1.0589742775147991E-4
8	0.0	0.0	0.0	5.294871387573996E-5	1.0589742775147991E-4
9	0.0	0.0	0.0	5.294871387573996E-5	1.0589742775147991E-4
10	0.0	0.0	0.0	2.1179485550295983E-4	1.0589742775147991E-4
11	0.0	0.0	0.0	2.1179485550295983E-4	1.0589742775147991E-4
12	0.0	0.0	0.0	2.1179485550295983E-4	2.1179485550295983E-4
13	0.0	0.0	0.0	2.1179485550295983E-4	3.706409971301797E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	80	7.796189E-9	15.425834	1
GTGTAAT	65	3.3996166E-6	14.604339	1
AACCGCG	265	0.0	13.98242	7
CGAACGT	150	0.0	13.929815	4
GAACCGT	85	2.6978887E-7	13.412644	6
GAACCGC	300	0.0	13.300872	6
CGGTCCA	465	0.0	13.075779	10
AAGACGG	450	0.0	12.875549	5
GGTCCAA	530	0.0	12.726576	11
CAAGACG	470	0.0	12.52875	4
ACGAACG	185	0.0	12.327094	15
AGAACCG	335	0.0	12.191921	5
TAGTACT	55	0.0030746723	12.087856	4
CGCATCG	355	0.0	12.04432	13
AATTCCG	190	0.0	11.997925	5
TAGACTG	80	2.8722015E-5	11.872946	5
CGCAAGA	465	0.0	11.842074	2
CGCGGTC	290	0.0	11.793565	10
GTCGGCG	275	0.0	11.747159	12
ATCGCCA	375	0.0	11.65657	16
>>END_MODULE