FastQCFastQC Report
Thu 2 Feb 2017
SRR4062517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888620
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26710.14142601476210143No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26190.13867268164056296No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23070.12215268291133209No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22740.12040537535343265No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21510.11389268354671665No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21170.1120924272749415No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA21040.11140409399455688No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC19840.10505024832946808No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG19350.1024557613495568No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT18960.10039076150840295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTG2550.019.84353344
CGCAATA7750.019.30309936
GTATCAA25300.019.0468831
TAGCGGC7800.018.8973130
CTAGCGG7850.018.77694729
TCTAGCG7800.018.61526128
TATACCG951.5979766E-518.5261945
ATAGCAC2300.018.1737923
CGTCGTA5350.018.09333810
ATACGAA8150.018.08577240
ATTACAC2350.017.7871153
TTAGCTA750.001291292517.5998824
ATACCGA750.001291292517.5998826
CAATACG8850.017.1524338
CGAATGC8600.017.13987543
GCAATAC8650.017.0403537
AATACGA8650.017.0403539
TACGAAT8800.016.75033241
CGGTCCA8950.016.71497310
ACCGTCG6050.016.363538
Produced by FastQC (version 0.11.5)