##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062517_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1888620
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.319566667725642	32.0	32.0	32.0	32.0	32.0
2	31.407015704588535	32.0	32.0	32.0	32.0	32.0
3	31.492856689011024	32.0	32.0	32.0	32.0	32.0
4	31.596566275905158	32.0	32.0	32.0	32.0	32.0
5	31.52169891243342	32.0	32.0	32.0	32.0	32.0
6	35.116951530747315	36.0	36.0	36.0	36.0	36.0
7	35.126369518484395	36.0	36.0	36.0	36.0	36.0
8	35.06947083055353	36.0	36.0	36.0	36.0	36.0
9	35.21508350012178	36.0	36.0	36.0	36.0	36.0
10	35.07049485867988	36.0	36.0	36.0	36.0	36.0
11	35.209825163346785	36.0	36.0	36.0	36.0	36.0
12	35.11484046552509	36.0	36.0	36.0	36.0	36.0
13	35.16911236776059	36.0	36.0	36.0	36.0	36.0
14	35.133792928169775	36.0	36.0	36.0	36.0	36.0
15	35.08502133833169	36.0	36.0	36.0	36.0	36.0
16	35.097274200209675	36.0	36.0	36.0	36.0	36.0
17	35.06452542067753	36.0	36.0	36.0	36.0	36.0
18	35.08097605659159	36.0	36.0	36.0	36.0	36.0
19	35.067532907625676	36.0	36.0	36.0	36.0	36.0
20	35.06916266903877	36.0	36.0	36.0	36.0	36.0
21	35.068061335790155	36.0	36.0	36.0	36.0	36.0
22	35.05704959176541	36.0	36.0	36.0	36.0	36.0
23	35.00927237877392	36.0	36.0	36.0	36.0	36.0
24	34.98606760491788	36.0	36.0	36.0	36.0	36.0
25	34.95508943037773	36.0	36.0	36.0	32.0	36.0
26	34.91939246645699	36.0	36.0	36.0	32.0	36.0
27	34.905124376528896	36.0	36.0	36.0	32.0	36.0
28	34.89572545032881	36.0	36.0	36.0	32.0	36.0
29	34.86988383052176	36.0	36.0	36.0	32.0	36.0
30	34.86112134786246	36.0	36.0	36.0	32.0	36.0
31	34.856884391778124	36.0	36.0	36.0	32.0	36.0
32	34.82844140165835	36.0	36.0	36.0	32.0	36.0
33	34.80288994080334	36.0	36.0	36.0	32.0	36.0
34	34.799320138513835	36.0	36.0	36.0	32.0	36.0
35	34.777930976056595	36.0	36.0	36.0	32.0	36.0
36	34.75428619838824	36.0	36.0	36.0	32.0	36.0
37	34.75109868581292	36.0	36.0	36.0	32.0	36.0
38	34.71526299626182	36.0	36.0	36.0	32.0	36.0
39	34.71457678092999	36.0	36.0	36.0	32.0	36.0
40	34.70791848016012	36.0	36.0	36.0	32.0	36.0
41	34.67770223761265	36.0	36.0	36.0	32.0	36.0
42	34.652494943397826	36.0	36.0	36.0	32.0	36.0
43	34.651399963994876	36.0	36.0	36.0	32.0	36.0
44	34.62200866240959	36.0	36.0	36.0	32.0	36.0
45	34.5813191642575	36.0	36.0	36.0	32.0	36.0
46	34.596008725948046	36.0	36.0	36.0	32.0	36.0
47	34.58062447713145	36.0	36.0	36.0	32.0	36.0
48	34.546048437483456	36.0	36.0	36.0	32.0	36.0
49	34.53452044349843	36.0	36.0	36.0	32.0	36.0
50	34.017942201183935	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	9.0
21	39.0
22	143.0
23	406.0
24	1024.0
25	2620.0
26	5314.0
27	10042.0
28	17041.0
29	27142.0
30	40257.0
31	58726.0
32	88626.0
33	148602.0
34	332939.0
35	1155687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.412917602674945	17.660397661859264	11.2968123291487	26.62987240631709
2	16.977018913422075	19.801350557808014	35.687481368010324	27.534149160759586
3	18.64529998252683	23.043455239567727	28.43456298547609	29.876681792429356
4	12.59434147085329	14.95130301627963	35.162219146487004	37.29213636638008
5	14.84724296046849	36.07983607078184	33.275989876205905	15.796931092543762
6	36.073476182508635	34.574332896328094	15.79678621124932	13.55540470991395
7	31.328271436286816	29.795617964439643	20.305672925204647	18.570437674068895
8	28.883576367930026	32.51352839639525	18.795416759326915	19.80747847634781
9	27.284419311454926	13.729813302834874	18.220499624064132	40.765267761646065
10	15.945028645254206	26.31090425813557	31.195105420889323	26.548961675720896
11	38.68856625472567	20.539865086677047	21.355963613643823	19.41560504495346
12	24.59690144126399	23.375692304433926	28.070813609937417	23.95659264436467
13	29.558195931420826	18.50441062786585	25.183467293579437	26.75392614713389
14	23.625398439071915	19.135718143406297	24.350054537175293	32.8888288803465
15	25.562050597790982	26.777806017091844	21.602069235738263	26.058074149378914
16	26.600745517891372	25.267179210216984	22.941512850652856	25.190562421238788
17	24.859738856943164	25.532081625737312	24.422011839332423	25.186167677987104
18	25.8391841662166	24.104266607364107	25.381018945049828	24.675530281369465
19	26.025563639059207	24.778462581143902	24.64291387362201	24.55305990617488
20	26.03657697154536	23.947538414291916	24.206457625144285	25.809426989018437
21	27.067435481992142	23.949550465419193	24.062807764399402	24.92020628818926
22	26.172125679067253	23.78165009371922	24.388707098304582	25.657517128908943
23	24.723501816140885	23.50377524329934	25.185532293420593	26.58719064713918
24	25.21926062415944	24.58377016022281	24.463735425866506	25.733233789751246
25	25.419300865181988	23.985820334424076	24.687973229130264	25.90690557126367
26	25.229797418220713	24.87414090711737	24.92454808272707	24.97151359193485
27	26.01222056316252	24.154514936832182	24.221018521460113	25.61224597854518
28	25.176266268492338	24.086264044646356	25.118922811364914	25.618546875496396
29	25.04426512480012	24.43932606876979	25.056602175133165	25.459806631296928
30	25.11796973451515	24.56560875136343	25.25664241615571	25.059779097965713
31	25.532081625737312	24.42444748017071	24.150967373002512	25.892503521089473
32	25.39483855937139	24.20878736855482	24.046605457953426	26.34976861412036
33	24.995181667037308	23.9999576410289	24.696127331067128	26.308733360866665
34	25.84506147345681	24.12422827249526	24.842954114644556	25.187756139403373
35	26.420561044572228	23.79769355402357	24.93275513337781	24.848990268026387
36	25.05702576484417	24.723660662282512	24.44372081202148	25.77559276085184
37	26.225445033940126	24.270843261217184	24.323421334095798	25.180290370746896
38	25.157522423780325	24.006629178977242	24.90951064798636	25.926337749256074
39	25.822928911056753	24.03352712562612	24.242462750579787	25.901081212737342
40	25.896686469485658	24.237114930478338	24.832046679586153	25.034151920449855
41	24.836388474123964	24.610826953013312	25.257171903294466	25.295612669568257
42	26.379419893890777	24.688979254693905	24.551630290900235	24.379970560515083
43	25.490199193061603	23.565248700109073	24.824527962215797	26.12002414461353
44	25.0464889707829	24.27153159449757	24.570744776609377	26.111234658110156
45	25.096896146392606	24.456799144348786	24.803930912518133	25.642373796740475
46	24.97843660202148	24.247372548272097	24.94169013622137	25.83250071348505
47	25.17776216378211	23.9952049619325	25.280323876864525	25.54670899742087
48	26.17493196090267	24.806313604642543	24.058624816003217	24.960129618451568
49	24.884624752464763	24.82500450064068	24.522667344410205	25.767703402484354
50	24.953272710521663	24.89788829715697	24.590150046224277	25.558688946097092
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	11.0
2	15.0
3	76.5
4	138.0
5	163.0
6	188.0
7	175.0
8	162.0
9	250.0
10	338.0
11	477.0
12	616.0
13	1151.0
14	1686.0
15	2133.0
16	2580.0
17	2962.0
18	3344.0
19	3607.0
20	3870.0
21	4653.0
22	5436.0
23	6129.0
24	6822.0
25	8215.0
26	9608.0
27	11832.0
28	14056.0
29	15982.0
30	17908.0
31	19915.5
32	21923.0
33	25805.5
34	29688.0
35	34635.5
36	39583.0
37	49334.5
38	59086.0
39	66847.5
40	74609.0
41	83624.0
42	92639.0
43	95609.5
44	98580.0
45	110384.5
46	122189.0
47	132037.5
48	141886.0
49	148016.5
50	154147.0
51	148655.0
52	143163.0
53	142188.0
54	141213.0
55	143877.0
56	146541.0
57	141966.0
58	137391.0
59	127219.0
60	117047.0
61	102542.5
62	88038.0
63	76328.0
64	64618.0
65	55044.5
66	45471.0
67	39167.0
68	32863.0
69	30162.0
70	27461.0
71	20996.0
72	14531.0
73	13339.0
74	12147.0
75	8560.5
76	4974.0
77	4552.5
78	4131.0
79	3626.0
80	3121.0
81	2477.0
82	1833.0
83	1615.0
84	1397.0
85	1163.0
86	929.0
87	696.0
88	463.0
89	288.0
90	113.0
91	75.0
92	37.0
93	26.5
94	16.0
95	13.0
96	10.0
97	7.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014931537312958668
2	0.003547563829674577
3	5.294871387573996E-4
4	1.0589742775147993E-4
5	0.0
6	4.235897110059197E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5884614162721988E-4
47	5.2948713875739963E-5
48	0.0
49	0.0
50	1.0589742775147993E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1888620.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.323187884892185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.23823633162577	40.66758890915519
2	12.120034578569909	12.44077623694667
3	3.47411857592139	5.349085212192227
4	1.5633112706439263	3.20936472263311
5	0.8410716939853	2.1583240287536047
6	0.5529304829501827	1.7026893038241837
7	0.3779987423901155	1.35800703331586
8	0.27769958479465456	1.140194237277808
9	0.21687003207612404	1.0017415262560958
>10	1.153303939775453	10.71462857424864
>50	0.08738379154014424	3.147212197989587
>100	0.08005706110245302	8.914576768137024
>500	0.011805891873168948	4.2080866669160555
>1k	0.005178022751389889	3.98772458235397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2671	0.14142601476210143	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2619	0.13867268164056296	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2307	0.12215268291133209	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2274	0.12040537535343265	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2151	0.11389268354671665	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2117	0.1120924272749415	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2104	0.11140409399455688	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1984	0.10505024832946808	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1935	0.1024557613495568	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1896	0.10039076150840295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0589742775147991E-4
2	0.0	0.0	0.0	0.0	1.0589742775147991E-4
3	0.0	0.0	0.0	0.0	1.0589742775147991E-4
4	0.0	0.0	0.0	0.0	1.0589742775147991E-4
5	0.0	0.0	0.0	0.0	2.1179485550295983E-4
6	0.0	0.0	0.0	0.0	2.647435693786998E-4
7	0.0	0.0	0.0	0.0	2.647435693786998E-4
8	0.0	0.0	0.0	5.294871387573996E-5	2.647435693786998E-4
9	0.0	0.0	0.0	5.294871387573996E-5	2.647435693786998E-4
10	0.0	0.0	0.0	1.0589742775147991E-4	2.647435693786998E-4
11	0.0	0.0	0.0	1.5884614162721988E-4	2.647435693786998E-4
12	0.0	0.0	0.0	1.5884614162721988E-4	4.765384248816596E-4
13	0.0	0.0	0.0	1.5884614162721988E-4	5.824358526331395E-4
14	0.0	0.0	0.0	1.5884614162721988E-4	6.353845665088795E-4
15	0.0	0.0	0.0	1.5884614162721988E-4	0.0010060255636390593
16	0.0	0.0	0.0	2.1179485550295983E-4	0.0010589742775147992
17	0.0	0.0	0.0	2.1179485550295983E-4	0.0010589742775147992
18	0.0	0.0	0.0	3.1769228325443977E-4	0.0010589742775147992
19	0.0	0.0	0.0	3.706409971301797E-4	0.0010589742775147992
20	0.0	0.0	0.0	4.2358971100591965E-4	0.001270769133017759
21	0.0	0.0	0.0	5.294871387573996E-4	0.001376666560769239
22	0.0	0.0	0.0	7.942307081360994E-4	0.001376666560769239
23	0.0	0.0	0.0	0.001270769133017759	0.001376666560769239
24	0.0	0.0	0.0	0.0018532049856508986	0.001376666560769239
25	0.0	0.0	0.0	0.002223845982781078	0.001376666560769239
26	0.0	0.0	0.0	0.0030710254047929177	0.001376666560769239
27	0.0	0.0	0.0	0.003865256112929017	0.0014296152746449788
28	0.0	0.0	0.0	0.007253973800976374	0.0014296152746449788
29	0.0	0.0	0.0	0.014560896315828489	0.0014296152746449788
30	0.0	0.0	0.0	0.029439484914911416	0.0014296152746449788
31	0.0	0.0	0.0	0.06295602079825481	0.0014825639885207187
32	0.0	0.0	0.0	0.09684319767872838	0.0015355127023964589
33	0.0	0.0	0.0	0.13380139996399487	0.0015355127023964589
34	0.0	0.0	0.0	0.17721934534210165	0.0015355127023964589
35	0.0	0.0	0.0	0.22587921339390665	0.0015355127023964589
36	0.0	0.0	0.0	0.29751882326778284	0.0015355127023964589
37	0.0	0.0	0.0	0.40886996854846397	0.0015355127023964589
38	0.0	0.0	0.0	0.5500841884550625	0.0016414101301479387
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGTG	255	0.0	19.843533	44
CGCAATA	775	0.0	19.303099	36
GTATCAA	2530	0.0	19.046883	1
TAGCGGC	780	0.0	18.89731	30
CTAGCGG	785	0.0	18.776947	29
TCTAGCG	780	0.0	18.615261	28
TATACCG	95	1.5979766E-5	18.526194	5
ATAGCAC	230	0.0	18.173792	3
CGTCGTA	535	0.0	18.093338	10
ATACGAA	815	0.0	18.085772	40
ATTACAC	235	0.0	17.787115	3
TTAGCTA	75	0.0012912925	17.599882	4
ATACCGA	75	0.0012912925	17.599882	6
CAATACG	885	0.0	17.15243	38
CGAATGC	860	0.0	17.139875	43
GCAATAC	865	0.0	17.04035	37
AATACGA	865	0.0	17.04035	39
TACGAAT	880	0.0	16.750332	41
CGGTCCA	895	0.0	16.714973	10
ACCGTCG	605	0.0	16.36353	8
>>END_MODULE