FastQCFastQC Report
Thu 2 Feb 2017
SRR4062516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670526
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG51260.3068494593918323No Hit
TCCATGTACTCTGCGTTGATACCAC43080.2578828464806893No Hit
GTATCAACGCAGAGTACTTTTTTTT30730.1839540360341593No Hit
GAGTACATGGAAGCAGTGGTATCAA22290.1334310271136157No Hit
TATCAACGCAGAGTACTTTTTTTTT21320.12762447277085182No Hit
CATGTACTCTGCGTTGATACCACTG19600.11732831455481686No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19430.11631067101020877No Hit
GCGCAAGACGGACCAGAGCGAAAGC18490.1106837008223757No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18390.11008508697260622No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18260.10930688896790591No Hit
GAATAACGCCGCCGCATCGCCAGTC17800.10655326525896634No Hit
GAATAGGACCGCGGTTCTATTTTGT17530.10493700786458876No Hit
GAACTACGACGGTATCTGATCGTCT17030.10194393861574139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT759.734158E-713.9256671
ACTCTAA2650.012.90629210
GATCTAT604.1113497E-412.65969851
CGACCAT2050.012.51280710
TAGGACT1900.012.4991124
CGCGTAA1155.320544E-912.39191110
CAAGACG3450.012.3904254
CGCATCG2850.012.33393713
CGAACGA1551.8189894E-1212.26050116
TTAGACC701.09171684E-412.2134184
TCGGTTG550.003064044312.09331217
ACGAACG1650.012.09186315
AAGACGG3700.012.0674345
TTTGAAC3350.011.9074623
CGGCTAA802.8661016E-511.8752259
TCGCGTA1201.0011718E-811.8752259
GTAATAC1052.7289934E-711.7589553
TCTAGAT2750.011.7418062
ATAACGA1701.8189894E-1211.73586812
CGGACAT1701.8189894E-1211.7351655
Produced by FastQC (version 0.11.5)