##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062516_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1670526
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.132767762968072	32.0	32.0	32.0	32.0	32.0
2	30.716623386885328	32.0	32.0	32.0	32.0	32.0
3	30.707343674986202	32.0	32.0	32.0	32.0	32.0
4	30.796164202173447	32.0	32.0	32.0	32.0	32.0
5	30.53864291845802	32.0	32.0	32.0	32.0	32.0
6	34.20963576741697	36.0	36.0	36.0	32.0	36.0
7	34.08190713583626	36.0	36.0	36.0	32.0	36.0
8	34.063447680550915	36.0	36.0	36.0	32.0	36.0
9	34.27157913136342	36.0	36.0	36.0	32.0	36.0
10	33.86466119054717	36.0	36.0	36.0	32.0	36.0
11	34.26145657116381	36.0	36.0	36.0	32.0	36.0
12	33.992759166873185	36.0	36.0	36.0	32.0	36.0
13	34.14031568499981	36.0	36.0	36.0	32.0	36.0
14	34.02389846072435	36.0	36.0	36.0	32.0	36.0
15	33.95713326221801	36.0	36.0	36.0	32.0	36.0
16	33.941766246080576	36.0	36.0	36.0	32.0	36.0
17	33.85829553086872	36.0	36.0	36.0	32.0	36.0
18	33.88512899529849	36.0	36.0	36.0	32.0	36.0
19	33.87623179764936	36.0	36.0	36.0	32.0	36.0
20	33.86997269123617	36.0	36.0	36.0	32.0	36.0
21	33.8419845006902	36.0	36.0	36.0	32.0	36.0
22	33.786983860173386	36.0	36.0	36.0	32.0	36.0
23	33.74245656757213	36.0	36.0	36.0	32.0	36.0
24	33.70875760089936	36.0	36.0	36.0	27.0	36.0
25	33.234426162777474	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	60.0
5	171.0
6	304.0
7	84.0
8	237.0
9	296.0
10	166.0
11	73.0
12	127.0
13	103.0
14	278.0
15	367.0
16	535.0
17	739.0
18	1115.0
19	1593.0
20	2450.0
21	3583.0
22	5511.0
23	8159.0
24	11292.0
25	16227.0
26	22102.0
27	28677.0
28	37969.0
29	50918.0
30	65037.0
31	87661.0
32	124315.0
33	177349.0
34	375978.0
35	647039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.469161286614685	16.829764459089255	10.766250311308646	25.93482394298741
2	16.975343377648365	19.667589364392168	36.61832879966285	26.738738458296616
3	19.399758624735696	23.446865114808897	28.15308411975853	29.000292140696875
4	12.667809394033192	14.877567918537867	35.46007149729415	36.994551190134786
5	14.753479882434947	36.265692706798795	33.56047485933155	15.42035255143471
6	34.956235832417526	34.73859780984522	16.627748548431047	13.677417809306212
7	30.5981812376713	30.180240892261008	20.595407747920333	18.626170122147364
8	28.637917904675287	32.58738258765221	19.362125234644946	19.41257427302756
9	27.217481600255017	14.21904969054634	18.41316692521881	40.150301783979835
10	15.757024737913328	26.631314233110302	31.615635780434538	25.996025248541834
11	37.94686308824587	20.926210054419485	21.557847673387194	19.569079183947448
12	24.282212045650713	23.732557303155268	28.622566414117195	23.362664237076817
13	29.667785436020377	19.017980221756066	25.261552292478274	26.05268204974528
14	23.609566934679087	19.350596809623273	24.84945653313994	32.190379722557694
15	25.509563816609166	26.716120321244997	22.35212895825505	25.422186903890793
16	26.232911355992744	25.571059753621483	23.617267111416883	24.57876177896889
17	24.297709877912098	25.747900771365384	25.246663110514422	24.707726240208096
18	25.350173468335736	24.570285245720836	26.086424873944747	23.993116411998685
19	25.468577200075526	25.498068387185718	24.964889303150823	24.068465109587933
20	25.380436541566624	24.174427232483737	25.31432838790802	25.13080783804162
21	26.522477442836102	24.465254504989158	24.093083029184502	24.919185022990234
22	25.468432901367244	24.558216498646544	24.73406057117848	25.23929002880773
23	24.31271251061054	24.22920693078329	25.204897395441183	26.25318316316499
24	24.475363090282087	25.368575074823962	24.974988745684847	25.181073089209104
25	25.115834952724953	24.4737526688493	24.768000421980886	25.64241195644486
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	208.0
1	208.0
2	539.0
3	870.0
4	870.0
5	870.0
6	2103.5
7	3337.0
8	3337.0
9	3337.0
10	3632.0
11	3927.0
12	3927.0
13	3927.0
14	4355.0
15	4783.0
16	4783.0
17	4783.0
18	7711.0
19	10639.0
20	10639.0
21	10639.0
22	16656.0
23	22673.0
24	22673.0
25	22673.0
26	33652.0
27	44631.0
28	44631.0
29	44631.0
30	55423.5
31	66216.0
32	66216.0
33	66216.0
34	82402.5
35	98589.0
36	98589.0
37	98589.0
38	112937.5
39	127286.0
40	127286.0
41	127286.0
42	149076.0
43	170866.0
44	170866.0
45	170866.0
46	201066.5
47	231267.0
48	231267.0
49	231267.0
50	228597.0
51	225927.0
52	225927.0
53	225927.0
54	209409.0
55	192891.0
56	192891.0
57	192891.0
58	178658.5
59	164426.0
60	164426.0
61	164426.0
62	145587.0
63	126748.0
64	126748.0
65	126748.0
66	104493.0
67	82238.0
68	82238.0
69	82238.0
70	62625.5
71	43013.0
72	43013.0
73	43013.0
74	33566.5
75	24120.0
76	24120.0
77	24120.0
78	19581.0
79	15042.0
80	15042.0
81	15042.0
82	10538.5
83	6035.0
84	6035.0
85	6035.0
86	4405.5
87	2776.0
88	2776.0
89	2776.0
90	1917.0
91	1058.0
92	1058.0
93	1058.0
94	634.5
95	211.0
96	211.0
97	211.0
98	480.0
99	749.0
100	749.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009458098826357685
2	0.003711405868570737
3	0.005866415727740844
4	0.015144930399167686
5	0.03843100915520022
6	0.0467517416669959
7	0.06878073133851254
8	0.09068999824007529
9	0.09679585950772392
10	0.11936360164403308
11	0.12008193826375646
12	0.13205421525914593
13	0.1212791659632954
14	0.12397292828725803
15	0.1133176017613614
16	0.11218023544679939
17	0.1230750075126038
18	0.13229366079905372
19	0.1335507498835696
20	0.12259611643278823
21	0.13223379941407676
22	0.15390362077573172
23	0.14211092793527308
24	0.1363043735925092
25	0.13193449248919203
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1670526.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.223050387697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69922593075087	41.099136412543544
2	12.591465829653764	13.151295089539476
3	3.8561376341378746	6.041378099084309
4	1.5507733993239312	3.239444694911753
5	0.8294706587084082	2.165874400242271
6	0.5339478244819778	1.6730630485394111
7	0.3477709725150903	1.2713162714723767
8	0.24889081830878138	1.0398270196459705
9	0.1871869450310836	0.8797925936049614
>10	0.9753230270223838	9.184602386111953
>50	0.0820981288506336	3.014031617904254
>100	0.08115607072257627	9.035543487824063
>500	0.011724872015578	4.115073852954184
>1k	0.004712938751359784	3.7819059480104102
>5k	1.1494972564292156E-4	0.30771507761113276
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5126	0.3068494593918323	No Hit
TCCATGTACTCTGCGTTGATACCAC	4308	0.2578828464806893	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3073	0.1839540360341593	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2229	0.1334310271136157	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2132	0.12762447277085182	No Hit
CATGTACTCTGCGTTGATACCACTG	1960	0.11732831455481686	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1943	0.11631067101020877	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1849	0.1106837008223757	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1839	0.11008508697260622	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1826	0.10930688896790591	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1780	0.10655326525896634	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1753	0.10493700786458876	No Hit
GAACTACGACGGTATCTGATCGTCT	1703	0.10194393861574139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.986138497694738E-5
7	0.0	0.0	0.0	0.0	5.986138497694738E-5
8	0.0	0.0	0.0	0.0	1.1972276995389477E-4
9	0.0	0.0	0.0	1.1972276995389477E-4	1.7958415493084213E-4
10	0.0	0.0	0.0	1.7958415493084213E-4	1.7958415493084213E-4
11	0.0	0.0	0.0	2.3944553990778953E-4	1.7958415493084213E-4
12	0.0	0.0	0.0	2.993069248847369E-4	1.7958415493084213E-4
13	0.0	0.0	0.0	2.993069248847369E-4	1.7958415493084213E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	75	9.734158E-7	13.925667	1
ACTCTAA	265	0.0	12.906292	10
GATCTAT	60	4.1113497E-4	12.6596985	1
CGACCAT	205	0.0	12.512807	10
TAGGACT	190	0.0	12.499112	4
CGCGTAA	115	5.320544E-9	12.391911	10
CAAGACG	345	0.0	12.390425	4
CGCATCG	285	0.0	12.333937	13
CGAACGA	155	1.8189894E-12	12.260501	16
TTAGACC	70	1.09171684E-4	12.213418	4
TCGGTTG	55	0.0030640443	12.093312	17
ACGAACG	165	0.0	12.091863	15
AAGACGG	370	0.0	12.067434	5
TTTGAAC	335	0.0	11.907462	3
CGGCTAA	80	2.8661016E-5	11.875225	9
TCGCGTA	120	1.0011718E-8	11.875225	9
GTAATAC	105	2.7289934E-7	11.758955	3
TCTAGAT	275	0.0	11.741806	2
ATAACGA	170	1.8189894E-12	11.735868	12
CGGACAT	170	1.8189894E-12	11.735165	5
>>END_MODULE