FastQCFastQC Report
Thu 2 Feb 2017
SRR4062516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670526
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21440.12834280939057519No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20030.1199023541088256No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC19600.11732831455481686No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18360.10990550281767539No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18180.10882799788809033No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17830.10673284941389717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA5700.018.52615436
ATACGAA5700.018.14019240
TAGGACG1701.2732926E-1018.1174874
GTATTAG5250.017.1854291
CGAGCCG7250.016.99295215
TTAACGG1702.4701876E-916.82338135
TACGAAT6300.016.76175741
ACCGTCG4100.016.6339998
CGCGGGA3650.016.27382944
TAGCGGC6400.015.8123630
CGAATGC6550.015.7861243
CAATACG6550.015.7861238
GCAATAC6700.015.76105537
CATGGGT10200.015.7449594
CGTCGTA4250.015.52927410
GTGTATA4000.015.4040131
GTTATAG4450.015.3298361
AATACGA6750.015.31838339
TTGATTA5750.015.30421336
GGCGTTA4750.015.28407542
Produced by FastQC (version 0.11.5)