##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062516_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1670526
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28847081697621	32.0	32.0	32.0	32.0	32.0
2	31.40955483482448	32.0	32.0	32.0	32.0	32.0
3	31.498640547947172	32.0	32.0	32.0	32.0	32.0
4	31.603303989282416	32.0	32.0	32.0	32.0	32.0
5	31.534085671219724	32.0	32.0	32.0	32.0	32.0
6	35.1333346502838	36.0	36.0	36.0	36.0	36.0
7	35.14800248544471	36.0	36.0	36.0	36.0	36.0
8	35.08853259392551	36.0	36.0	36.0	36.0	36.0
9	35.22456459821637	36.0	36.0	36.0	36.0	36.0
10	35.09912686183873	36.0	36.0	36.0	36.0	36.0
11	35.228808770411234	36.0	36.0	36.0	36.0	36.0
12	35.12677084942108	36.0	36.0	36.0	36.0	36.0
13	35.18066944184047	36.0	36.0	36.0	36.0	36.0
14	35.13943153234371	36.0	36.0	36.0	36.0	36.0
15	35.09862342759107	36.0	36.0	36.0	36.0	36.0
16	35.11629211398087	36.0	36.0	36.0	36.0	36.0
17	35.084899606471254	36.0	36.0	36.0	36.0	36.0
18	35.095830295368046	36.0	36.0	36.0	36.0	36.0
19	35.07616283733387	36.0	36.0	36.0	36.0	36.0
20	35.07260108492774	36.0	36.0	36.0	36.0	36.0
21	35.06215287879387	36.0	36.0	36.0	36.0	36.0
22	35.044333940327775	36.0	36.0	36.0	36.0	36.0
23	35.0019532769918	36.0	36.0	36.0	36.0	36.0
24	34.974272175350755	36.0	36.0	36.0	36.0	36.0
25	34.94239778369208	36.0	36.0	36.0	32.0	36.0
26	34.89521863173635	36.0	36.0	36.0	32.0	36.0
27	34.881555270615365	36.0	36.0	36.0	32.0	36.0
28	34.85910844847671	36.0	36.0	36.0	32.0	36.0
29	34.83455031528992	36.0	36.0	36.0	32.0	36.0
30	34.82206921652222	36.0	36.0	36.0	32.0	36.0
31	34.81812794293534	36.0	36.0	36.0	32.0	36.0
32	34.78266905154425	36.0	36.0	36.0	32.0	36.0
33	34.75793492588562	36.0	36.0	36.0	32.0	36.0
34	34.74050029751108	36.0	36.0	36.0	32.0	36.0
35	34.704303913857075	36.0	36.0	36.0	32.0	36.0
36	34.68248503764683	36.0	36.0	36.0	32.0	36.0
37	34.66202262042015	36.0	36.0	36.0	32.0	36.0
38	34.61937377807948	36.0	36.0	36.0	32.0	36.0
39	34.58976873152528	36.0	36.0	36.0	32.0	36.0
40	34.563274082534484	36.0	36.0	36.0	32.0	36.0
41	34.53717751175378	36.0	36.0	36.0	32.0	36.0
42	34.48171114966184	36.0	36.0	36.0	32.0	36.0
43	34.49166190768656	36.0	36.0	36.0	32.0	36.0
44	34.462145455982125	36.0	36.0	36.0	32.0	36.0
45	34.38553365826093	36.0	36.0	36.0	32.0	36.0
46	34.42301825891965	36.0	36.0	36.0	32.0	36.0
47	34.374361129368836	36.0	36.0	36.0	32.0	36.0
48	34.35248837791211	36.0	36.0	36.0	32.0	36.0
49	34.34908046926537	36.0	36.0	36.0	32.0	36.0
50	33.824971895079756	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	10.0
21	33.0
22	158.0
23	434.0
24	1080.0
25	2652.0
26	5688.0
27	10547.0
28	17959.0
29	26858.0
30	38147.0
31	54883.0
32	81528.0
33	129397.0
34	284723.0
35	1016427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.867647701548904	17.434353703008544	11.296988463590171	26.40101013185238
2	16.322496487458714	20.087366046012946	36.286302495805	27.303834970723333
3	18.781558457262477	23.622771714762727	28.519297388643356	29.07637243933144
4	12.427896875471408	15.304419451621168	35.549444365959424	36.718239306948
5	14.428629066533535	36.61170194298084	33.41558287629166	15.544086114193972
6	35.290927375906904	34.88141417043795	16.33724828197208	13.490410171683068
7	30.576435551697816	30.226844854162614	20.550186318672274	18.64653327546729
8	28.600632375670898	32.73729352311786	19.241843586989965	19.42023051422127
9	27.193590521787748	13.963745550802562	18.267479823720194	40.5751841036895
10	15.661115121823904	26.691952115680927	31.652126336255765	25.994806426239396
11	38.223110565175276	20.761245260474844	21.502089760949545	19.513554413400332
12	24.257090281743597	23.61244302692685	28.68324108693908	23.44722560439047
13	29.83365718342606	18.933737038513616	25.206192540553097	26.026413237507228
14	23.61806999711468	19.231846735698817	24.862767774940348	32.28731549224616
15	25.62396514630721	26.702128551127007	22.228028776564987	25.445877526000793
16	26.279686757344695	25.49071370334853	23.600171443006573	24.629428096300206
17	24.417339209326883	25.657307937739372	25.162793036444807	24.76255981648894
18	25.422112556164944	24.323177250758143	26.25981277753235	23.994897415544564
19	25.498016792315713	25.39840744771407	25.000448960387327	24.103126799582885
20	25.494425109217094	24.047335988784372	25.378054576821913	25.080184325176617
21	26.71463958058719	24.318986953809755	24.119768264606478	24.84660520099657
22	25.491791208278116	24.465168455923465	24.822540924235838	25.220499411562585
23	24.362566042072974	24.01088040533341	25.279163568839998	26.347389983753622
24	24.64056231390592	25.232052658863136	24.886592606161173	25.24079242106977
25	25.329866161915472	24.216923292424063	24.85606329982293	25.597147245837537
26	24.64565053162896	25.002424386091565	25.420196991845685	24.93172809043379
27	25.780921697716767	24.67013383808453	24.657802392779278	24.89114207141942
28	24.719878649000375	24.25721000451355	25.540398652879393	25.482512693606687
29	24.596863502872747	24.74819308409447	25.586671503466572	25.06827190956621
30	24.84385157728763	24.76052452939972	25.708249976354754	24.687373916957892
31	24.99859325745304	24.700723005807752	24.628051284445736	25.67263245229347
32	24.832058884447175	24.787102984329486	24.667080907450707	25.71375722377263
33	24.400997051228178	24.379686398176386	25.28431164794801	25.935004902647428
34	25.071025533275147	24.6099731461827	25.639529106401216	24.679472214140937
35	25.946857456872863	24.339279963316944	25.290956261680453	24.42290631812974
36	24.684201263554115	25.13453846273569	24.960042525527886	25.221217748182305
37	25.920279001943104	24.6691760559249	24.859295814611684	24.551249127520315
38	24.77429264794442	24.466365683623003	25.13986612599864	25.61947554243394
39	25.220379688792633	24.3993807938338	24.98350818843885	25.39673132893472
40	25.687897105462593	24.524610811205573	25.00781191073949	24.779680172592343
41	24.258107925288204	24.946753298063005	25.790798826237964	25.00433995041083
42	25.907887695252874	25.039538444777275	25.071803731279847	23.980770128690004
43	25.08317739442547	23.778797815777786	25.222115668956963	25.915909120839782
44	24.721435045009777	24.575313404281047	25.325316696657218	25.377934854051958
45	24.87701478456486	24.809491142310865	25.162194422595043	25.151299650529236
46	24.469777782566688	24.51563160345903	25.565540434569712	25.44905017940457
47	24.868828661648283	24.20813816015923	25.91077655228147	25.012256625911018
48	25.409122635624946	25.402537883277482	24.56567572129976	24.62266375979781
49	24.389802972237487	25.0134987423123	25.355127666375743	25.241570619074473
50	24.31018733021815	25.616063443490255	24.651816254281584	25.421932972010016
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	19.0
2	16.0
3	83.5
4	151.0
5	189.0
6	227.0
7	221.0
8	215.0
9	284.5
10	354.0
11	564.0
12	774.0
13	1394.0
14	2014.0
15	2694.0
16	3374.0
17	3910.0
18	4446.0
19	4724.5
20	5003.0
21	5870.0
22	6737.0
23	7347.5
24	7958.0
25	9359.5
26	10761.0
27	13223.5
28	15686.0
29	17917.5
30	20149.0
31	22235.0
32	24321.0
33	27760.0
34	31199.0
35	36081.5
36	40964.0
37	49237.5
38	57511.0
39	63174.0
40	68837.0
41	75718.5
42	82600.0
43	86289.5
44	89979.0
45	101158.5
46	112338.0
47	121723.5
48	131109.0
49	133363.5
50	135618.0
51	128404.5
52	121191.0
53	118673.0
54	116155.0
55	117586.5
56	119018.0
57	115381.5
58	111745.0
59	103353.5
60	94962.0
61	83464.0
62	71966.0
63	62590.5
64	53215.0
65	45626.5
66	38038.0
67	33405.0
68	28772.0
69	26105.5
70	23439.0
71	18302.0
72	13165.0
73	11874.0
74	10583.0
75	7767.5
76	4952.0
77	4482.5
78	4013.0
79	3385.5
80	2758.0
81	2228.5
82	1699.0
83	1441.0
84	1183.0
85	975.5
86	768.0
87	584.5
88	401.0
89	244.0
90	87.0
91	58.0
92	29.0
93	18.0
94	7.0
95	7.0
96	7.0
97	6.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014905484859259896
2	0.004968494953086633
3	8.979207746542106E-4
4	1.1972276995389475E-4
5	0.0
6	3.591683098616843E-4
7	5.9861384976947376E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	5.9861384976947376E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1670526.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.20201045572385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.12339823214823	43.67770656503449
2	12.650087784431335	13.966205562840065
3	3.684679037283322	6.102050722263014
4	1.5023439007529413	3.3172961486982726
5	0.7838810247346952	2.1635904261724086
6	0.4829762527860072	1.5996756093695699
7	0.31203199937411064	1.2057355584379053
8	0.22994730478616202	1.0154842818456988
9	0.17372770747010224	0.8631106851792167
>10	0.8972629988882685	8.879367160209494
>50	0.07541093557511674	2.8762538479617645
>100	0.07170253747772384	8.440799740664616
>500	0.00971282871290767	3.6510006296474664
>1k	0.0028374555790516786	2.2417230616759927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2144	0.12834280939057519	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2003	0.1199023541088256	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1960	0.11732831455481686	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1836	0.10990550281767539	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1818	0.10882799788809033	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1783	0.10673284941389717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.986138497694738E-5
2	0.0	0.0	0.0	0.0	5.986138497694738E-5
3	0.0	0.0	0.0	0.0	5.986138497694738E-5
4	0.0	0.0	0.0	0.0	5.986138497694738E-5
5	0.0	0.0	0.0	0.0	5.986138497694738E-5
6	0.0	0.0	0.0	0.0	5.986138497694738E-5
7	0.0	0.0	0.0	0.0	5.986138497694738E-5
8	0.0	0.0	0.0	0.0	5.986138497694738E-5
9	0.0	0.0	0.0	5.986138497694738E-5	1.1972276995389477E-4
10	0.0	0.0	0.0	1.1972276995389477E-4	1.1972276995389477E-4
11	0.0	0.0	0.0	1.7958415493084213E-4	1.1972276995389477E-4
12	0.0	0.0	0.0	2.3944553990778953E-4	1.1972276995389477E-4
13	0.0	0.0	0.0	2.3944553990778953E-4	1.1972276995389477E-4
14	0.0	0.0	0.0	2.3944553990778953E-4	1.7958415493084213E-4
15	0.0	0.0	0.0	2.3944553990778953E-4	2.3944553990778953E-4
16	0.0	0.0	0.0	2.3944553990778953E-4	2.993069248847369E-4
17	0.0	0.0	0.0	2.3944553990778953E-4	2.993069248847369E-4
18	0.0	0.0	0.0	2.3944553990778953E-4	2.993069248847369E-4
19	0.0	0.0	0.0	4.190296948386317E-4	2.993069248847369E-4
20	0.0	0.0	0.0	4.7889107981557907E-4	2.993069248847369E-4
21	0.0	0.0	0.0	9.577821596311581E-4	3.5916830986168426E-4
22	0.0	0.0	0.0	0.001436673239446737	3.5916830986168426E-4
23	0.0	0.0	0.0	0.0022747326291240005	4.7889107981557907E-4
24	0.0	0.0	0.0	0.003591683098616843	4.7889107981557907E-4
25	0.0	0.0	0.0	0.004190296948386317	4.7889107981557907E-4
26	0.0	0.0	0.0	0.0055671088028561065	5.986138497694738E-4
27	0.0	0.0	0.0	0.007243227582210633	5.986138497694738E-4
28	0.0	0.0	0.0	0.012630752230135897	5.986138497694738E-4
29	0.0	0.0	0.0	0.023585385680917267	5.986138497694738E-4
30	0.0	0.0	0.0	0.039867682394646956	5.986138497694738E-4
31	0.0	0.0	0.0	0.0799748103292017	5.986138497694738E-4
32	0.0	0.0	0.0	0.12834280939057519	6.584752347464211E-4
33	0.0	0.0	0.0	0.175214273827525	6.584752347464211E-4
34	0.0	0.0	0.0	0.23010716385138574	6.584752347464211E-4
35	0.0	0.0	0.0	0.2951764893213275	6.584752347464211E-4
36	0.0	0.0	0.0	0.3907152597445355	6.584752347464211E-4
37	0.0	0.0	0.0	0.5296535342760305	6.584752347464211E-4
38	0.0	0.0	0.0	0.7119913129158122	6.584752347464211E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	570	0.0	18.526154	36
ATACGAA	570	0.0	18.140192	40
TAGGACG	170	1.2732926E-10	18.117487	4
GTATTAG	525	0.0	17.185429	1
CGAGCCG	725	0.0	16.992952	15
TTAACGG	170	2.4701876E-9	16.823381	35
TACGAAT	630	0.0	16.761757	41
ACCGTCG	410	0.0	16.633999	8
CGCGGGA	365	0.0	16.273829	44
TAGCGGC	640	0.0	15.81236	30
CGAATGC	655	0.0	15.78612	43
CAATACG	655	0.0	15.78612	38
GCAATAC	670	0.0	15.761055	37
CATGGGT	1020	0.0	15.744959	4
CGTCGTA	425	0.0	15.529274	10
GTGTATA	400	0.0	15.404013	1
GTTATAG	445	0.0	15.329836	1
AATACGA	675	0.0	15.318383	39
TTGATTA	575	0.0	15.304213	36
GGCGTTA	475	0.0	15.284075	42
>>END_MODULE