FastQCFastQC Report
Thu 2 Feb 2017
SRR4062515_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062515_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127571
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38660.18170956456917303No Hit
GCGCAAGACGGACCAGAGCGAAAGC23320.10960856300447788No Hit
TATCAACGCAGAGTACTTTTTTTTT23240.10923254735094622No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21560.10133621862678144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1650.015.5458017
AGAACCG2150.013.69572455
GTTATTC2050.013.4336333
GCGTTAT1800.012.6577131
ACGGTAT3700.012.5813629
CGAACGA1900.012.5038116
GAACCGC2150.012.3717876
TAGATCG550.003072359612.0891875
TCGGACC550.003073984312.0883334
CGTTATT1850.011.8041292
CGGTCCA4200.011.76216810
TAATGCA1052.7290116E-711.7593994
CGCATCG4050.011.73031313
CGCAAGA4350.011.5681642
AAGACGG4400.011.4415515
TAGAACC2600.011.324514
TTTGAAC3550.011.2349283
GCATCGC4450.011.10346114
CGCCAGT4200.011.08775618
ACAGCGA600.005875885511.0835818
Produced by FastQC (version 0.11.5)