##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062515_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2127571
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.141855195431784	32.0	32.0	32.0	32.0	32.0
2	30.79317587991188	32.0	32.0	32.0	32.0	32.0
3	30.774727141890917	32.0	32.0	32.0	32.0	32.0
4	30.8488882392174	32.0	32.0	32.0	32.0	32.0
5	30.643249508477037	32.0	32.0	32.0	32.0	32.0
6	34.27405571893958	36.0	36.0	36.0	32.0	36.0
7	34.15654471695657	36.0	36.0	36.0	32.0	36.0
8	34.16474749843836	36.0	36.0	36.0	32.0	36.0
9	34.332748472318904	36.0	36.0	36.0	32.0	36.0
10	33.981552672037736	36.0	36.0	36.0	32.0	36.0
11	34.29718961200355	36.0	36.0	36.0	32.0	36.0
12	34.07463487704993	36.0	36.0	36.0	32.0	36.0
13	34.20064900301799	36.0	36.0	36.0	32.0	36.0
14	34.10830002853019	36.0	36.0	36.0	32.0	36.0
15	34.04348856042877	36.0	36.0	36.0	32.0	36.0
16	34.049221389086426	36.0	36.0	36.0	32.0	36.0
17	33.969776331788694	36.0	36.0	36.0	32.0	36.0
18	33.97975437717472	36.0	36.0	36.0	32.0	36.0
19	33.96767205418762	36.0	36.0	36.0	32.0	36.0
20	33.95792008821327	36.0	36.0	36.0	32.0	36.0
21	33.93954279316648	36.0	36.0	36.0	32.0	36.0
22	33.88685500977406	36.0	36.0	36.0	32.0	36.0
23	33.852541701311026	36.0	36.0	36.0	32.0	36.0
24	33.82542110228049	36.0	36.0	36.0	32.0	36.0
25	33.36992796010098	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	14.0
4	75.0
5	207.0
6	395.0
7	101.0
8	316.0
9	370.0
10	223.0
11	74.0
12	175.0
13	117.0
14	277.0
15	364.0
16	619.0
17	808.0
18	1136.0
19	1678.0
20	2762.0
21	4265.0
22	6468.0
23	9859.0
24	13732.0
25	19371.0
26	26898.0
27	34520.0
28	46491.0
29	61628.0
30	78491.0
31	107603.0
32	153232.0
33	218162.0
34	477687.0
35	859451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.71833897640627	17.281246900540427	10.769502680988461	26.23091144206484
2	17.292206424829942	19.96878019982176	36.4847689490368	26.254244426311494
3	19.636704630382567	23.526912247881288	28.07240525130789	28.763977870428253
4	12.966530434185797	15.159130631812213	35.70137163261795	36.17296730138404
5	14.894069211187313	36.12019375897489	33.41256160762397	15.573175422213822
6	34.20698093590456	35.123342219346426	16.858658784150762	13.81101806059825
7	30.194848716741507	30.195272024837838	20.73838101585477	18.871498242565888
8	28.25308068733411	32.44485717861071	19.621322675006656	19.68073945904852
9	27.57383504703566	14.009922301183478	18.56618278393707	39.8500598678438
10	16.18811881188119	26.300634341000638	31.427879004630498	26.08336784248767
11	37.772535422369614	21.101468753632354	21.980527990836702	19.145467833161334
12	24.64680484161401	23.640566919528265	28.67109762766938	23.04153061118835
13	29.707654848057885	19.302206071284367	25.138897124361282	25.851241956296466
14	23.414981013608365	19.780248223128808	24.721792537493947	32.082978225768876
15	25.46026574361769	27.03348640624978	22.088285380995337	25.417962469137194
16	25.918790941092823	25.589497622999147	23.707848408376417	24.783863027531613
17	24.218640807949992	25.510086433632644	25.099918446406395	25.17135431201097
18	24.980597854350954	24.74198323108673	26.199085545930963	24.078333368631355
19	25.77445253791136	24.80558971629253	25.015448810900438	24.404508934895674
20	25.584461848978986	24.350243695379127	24.994105851842058	25.071188603799833
21	26.893786971526897	24.001954087417175	24.10342355927234	25.00083538178359
22	25.85168604911161	24.50411773982098	24.680219079292677	24.963977131774737
23	24.61911528466864	24.194657517934022	25.214523440489543	25.971703756907793
24	24.713919067559484	25.162790675783103	24.98455047652308	25.13873978013434
25	24.90708401713316	24.557398945485367	25.199962913615554	25.335554123765924
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	152.0
2	553.0
3	954.0
4	954.0
5	954.0
6	2483.5
7	4013.0
8	4013.0
9	4013.0
10	4312.0
11	4611.0
12	4611.0
13	4611.0
14	5436.5
15	6262.0
16	6262.0
17	6262.0
18	10096.5
19	13931.0
20	13931.0
21	13931.0
22	22238.5
23	30546.0
24	30546.0
25	30546.0
26	45665.0
27	60784.0
28	60784.0
29	60784.0
30	76637.5
31	92491.0
32	92491.0
33	92491.0
34	112435.0
35	132379.0
36	132379.0
37	132379.0
38	149827.0
39	167275.0
40	167275.0
41	167275.0
42	192347.0
43	217419.0
44	217419.0
45	217419.0
46	245809.0
47	274199.0
48	274199.0
49	274199.0
50	277275.0
51	280351.0
52	280351.0
53	280351.0
54	260212.0
55	240073.0
56	240073.0
57	240073.0
58	221250.5
59	202428.0
60	202428.0
61	202428.0
62	181439.0
63	160450.0
64	160450.0
65	160450.0
66	134958.0
67	109466.0
68	109466.0
69	109466.0
70	84123.5
71	58781.0
72	58781.0
73	58781.0
74	46439.0
75	34097.0
76	34097.0
77	34097.0
78	27515.0
79	20933.0
80	20933.0
81	20933.0
82	14982.0
83	9031.0
84	9031.0
85	9031.0
86	6635.5
87	4240.0
88	4240.0
89	4240.0
90	2864.0
91	1488.0
92	1488.0
93	1488.0
94	868.0
95	248.0
96	248.0
97	248.0
98	608.5
99	969.0
100	969.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008648360031228099
2	0.00352514675185928
3	0.004747197625837163
4	0.015275635924723547
5	0.03783657513662293
6	0.04737797234498872
7	0.06862285676952731
8	0.08972673532399154
9	0.09663602295763572
10	0.11896195238607783
11	0.12088903261042758
12	0.1327805276533662
13	0.12300412066154315
14	0.12352114218514916
15	0.11468477432715525
16	0.11346272345317736
17	0.12164106391749088
18	0.13188749047622852
19	0.13216950221637727
20	0.12135905217734214
21	0.13132346699593103
22	0.1516283122866405
23	0.13997182702715916
24	0.13677569397214007
25	0.1316994826494627
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2127571.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.75994125797152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0321371085878	40.116666371238686
2	12.73809636045411	12.931700459900627
3	3.5672246739746125	5.432163447148138
4	1.4499737790562335	2.9440233540197354
5	0.7662295039210059	1.944688230457746
6	0.4830326960206028	1.4711226765411247
7	0.31399167818629514	1.115673939815977
8	0.23384933973342728	0.9496142998467355
9	0.16444063378187043	0.7512297220072216
>10	1.0279531213302666	9.829890811693895
>50	0.1084439548626388	3.866888399188494
>100	0.09736579590506392	10.555049456505634
>500	0.01252840223204179	4.371034207711171
>1k	0.004732951954326898	3.7202546239248813
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3866	0.18170956456917303	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2332	0.10960856300447788	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2324	0.10923254735094622	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2156	0.10133621862678144	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.400391338291413E-5	0.0
8	0.0	0.0	0.0	9.400391338291413E-5	4.7001956691457065E-5
9	0.0	0.0	0.0	1.410058700743712E-4	4.7001956691457065E-5
10	0.0	0.0	0.0	1.410058700743712E-4	4.7001956691457065E-5
11	0.0	0.0	0.0	1.8800782676582826E-4	4.7001956691457065E-5
12	0.0	0.0	0.0	1.8800782676582826E-4	9.400391338291413E-5
13	0.0	0.0	0.0	1.8800782676582826E-4	1.410058700743712E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	165	0.0	15.545801	7
AGAACCG	215	0.0	13.6957245	5
GTTATTC	205	0.0	13.433633	3
GCGTTAT	180	0.0	12.657713	1
ACGGTAT	370	0.0	12.581362	9
CGAACGA	190	0.0	12.50381	16
GAACCGC	215	0.0	12.371787	6
TAGATCG	55	0.0030723596	12.089187	5
TCGGACC	55	0.0030739843	12.088333	4
CGTTATT	185	0.0	11.804129	2
CGGTCCA	420	0.0	11.762168	10
TAATGCA	105	2.7290116E-7	11.759399	4
CGCATCG	405	0.0	11.730313	13
CGCAAGA	435	0.0	11.568164	2
AAGACGG	440	0.0	11.441551	5
TAGAACC	260	0.0	11.32451	4
TTTGAAC	355	0.0	11.234928	3
GCATCGC	445	0.0	11.103461	14
CGCCAGT	420	0.0	11.087756	18
ACAGCGA	60	0.0058758855	11.083581	8
>>END_MODULE