FastQCFastQC Report
Thu 2 Feb 2017
SRR4062515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127571
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22680.10660043777622463No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC22470.10561339668570403No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22360.105096375162098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA701.1329957E-625.14273541
GTATCAA40950.019.0213531
TATACGG600.00741411418.3332445
GCATATA2650.018.2670691
GCGCAAA5400.017.11102922
TACCGTT902.2164488E-417.1110297
CGCGCAA5450.016.55037921
TAGTACG1753.6288839E-916.3435462
CCTTATA2850.016.211212
AACGCAG47900.016.1210086
GTCCTAA5500.016.0025561
CGACGGT7250.015.7792357
TAGGACG5750.015.6868814
GTAGAAC8300.015.6384793
CGCAAAT5850.015.4187323
TATACCG1004.9460714E-415.3999265
GTGTACG1158.211017E-515.3067921
TATGGCG7100.015.18302516
ATACCGT6100.015.1474676
GACGGTA7300.015.0684218
Produced by FastQC (version 0.11.5)