##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062515_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2127571
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298192633759342	32.0	32.0	32.0	32.0	32.0
2	31.385695236492694	32.0	32.0	32.0	32.0	32.0
3	31.472834044081257	32.0	32.0	32.0	32.0	32.0
4	31.57859361685227	32.0	32.0	32.0	32.0	32.0
5	31.507875412853437	32.0	32.0	32.0	32.0	32.0
6	35.089936364050835	36.0	36.0	36.0	36.0	36.0
7	35.10523644099304	36.0	36.0	36.0	36.0	36.0
8	35.04344249851121	36.0	36.0	36.0	36.0	36.0
9	35.18375461970482	36.0	36.0	36.0	36.0	36.0
10	35.04451508316291	36.0	36.0	36.0	36.0	36.0
11	35.18848442660668	36.0	36.0	36.0	36.0	36.0
12	35.09066630443825	36.0	36.0	36.0	36.0	36.0
13	35.138068247781156	36.0	36.0	36.0	36.0	36.0
14	35.100465742388856	36.0	36.0	36.0	36.0	36.0
15	35.061169756496966	36.0	36.0	36.0	36.0	36.0
16	35.0715412082605	36.0	36.0	36.0	36.0	36.0
17	35.04649151544179	36.0	36.0	36.0	36.0	36.0
18	35.057321236283066	36.0	36.0	36.0	36.0	36.0
19	35.04243712665758	36.0	36.0	36.0	36.0	36.0
20	35.03702344128586	36.0	36.0	36.0	36.0	36.0
21	35.03520258548363	36.0	36.0	36.0	36.0	36.0
22	35.014250053229716	36.0	36.0	36.0	36.0	36.0
23	34.96982568384322	36.0	36.0	36.0	32.0	36.0
24	34.952328735445256	36.0	36.0	36.0	32.0	36.0
25	34.92393626346665	36.0	36.0	36.0	32.0	36.0
26	34.883115064080116	36.0	36.0	36.0	32.0	36.0
27	34.864145544379014	36.0	36.0	36.0	32.0	36.0
28	34.853414527646784	36.0	36.0	36.0	32.0	36.0
29	34.82481665711744	36.0	36.0	36.0	32.0	36.0
30	34.81186620799024	36.0	36.0	36.0	32.0	36.0
31	34.81257593753628	36.0	36.0	36.0	32.0	36.0
32	34.77301768072605	36.0	36.0	36.0	32.0	36.0
33	34.76310120790328	36.0	36.0	36.0	32.0	36.0
34	34.74832990297386	36.0	36.0	36.0	32.0	36.0
35	34.72584886708834	36.0	36.0	36.0	32.0	36.0
36	34.695109117392555	36.0	36.0	36.0	32.0	36.0
37	34.683273084658516	36.0	36.0	36.0	32.0	36.0
38	34.65080601305432	36.0	36.0	36.0	32.0	36.0
39	34.64425958052634	36.0	36.0	36.0	32.0	36.0
40	34.63555011795141	36.0	36.0	36.0	32.0	36.0
41	34.60834726549666	36.0	36.0	36.0	32.0	36.0
42	34.586387951330416	36.0	36.0	36.0	32.0	36.0
43	34.57399212529218	36.0	36.0	36.0	32.0	36.0
44	34.5466078452846	36.0	36.0	36.0	32.0	36.0
45	34.50586090898964	36.0	36.0	36.0	32.0	36.0
46	34.5099627697501	36.0	36.0	36.0	32.0	36.0
47	34.48498075974903	36.0	36.0	36.0	32.0	36.0
48	34.44482087789315	36.0	36.0	36.0	32.0	36.0
49	34.43065354810721	36.0	36.0	36.0	32.0	36.0
50	33.90611876172405	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	13.0
21	48.0
22	152.0
23	472.0
24	1342.0
25	3060.0
26	6475.0
27	12204.0
28	21033.0
29	32762.0
30	48380.0
31	70188.0
32	104134.0
33	171613.0
34	378889.0
35	1276805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.108748547424476	17.85873444474948	11.281481871481382	26.751035136344665
2	16.67461819726972	20.504608049079824	36.10353691915244	26.717236834498014
3	18.94332574091848	23.87808204884276	28.443401414388862	28.735190795849896
4	12.599356354600017	15.729172590877194	35.88283153213832	35.78863952238447
5	14.61859557213367	36.40588257689167	33.22464914214379	15.750872708830869
6	34.37571384389486	35.4228258958829	16.540732461413654	13.660727798808592
7	30.202202512725783	30.232189775189532	20.710622917224818	18.854984794859863
8	28.25536727093949	32.6199689693082	19.512580308718253	19.612083451034064
9	27.713199700503534	13.866188249416822	18.38472135595005	40.03589069412959
10	16.11509087123297	26.429999280870064	31.401349238168784	26.053560609728184
11	37.9996249243856	20.976644257700446	21.912970236950965	19.110760580962985
12	24.61609976823335	23.584312814942486	28.600784650665005	23.198802766159154
13	29.756844777448084	19.185117676448872	25.187784567471542	25.870252978631502
14	23.531764627361436	19.626795063478493	24.691302898939682	32.15013741022039
15	25.494707344666757	26.9893225654984	21.942957485320115	25.573012604514727
16	25.98667682535624	25.58321202911677	23.621961382252344	24.808149763274645
17	24.35476888902885	25.53324894915375	24.96447827123043	25.147503890586965
18	25.149054955157784	24.64895413596068	26.13304091849344	24.0689499903881
19	25.931637534070546	24.750666370240992	24.99535855677672	24.322337538911746
20	25.749786963631294	24.27928374658237	24.845281309060894	25.125647980725436
21	27.03862761806774	23.948483975387898	24.040513806589768	24.9723745999546
22	25.92477524839359	24.42090064209373	24.60998951386346	25.044334595649214
23	24.75898571657538	24.001455180579168	25.23671360438735	26.0028454984581
24	24.99860169178843	24.982433018686567	24.867184220879114	25.15178106864589
25	25.130724192048113	24.349739679662864	25.14891394928771	25.370622179001312
26	24.578263193096728	25.166398677176932	25.537479125255985	24.717859004470355
27	25.636606251918266	24.53539740859412	24.759220726358837	25.068775613128775
28	24.50127398803612	24.723969258840246	25.480277743962482	25.29447900916115
29	24.699246229620538	24.673677165180386	25.177491138956114	25.449585466242958
30	24.547899929074045	25.08485028231725	25.570380494940004	24.796869293668696
31	25.306887525727696	24.599508077521268	24.541319655137244	25.552284741613796
32	24.982621026513332	24.772851293799363	24.631563411984843	25.612964267702466
33	24.625500159571644	24.275241578306904	25.251190207048317	25.84806805507313
34	25.21838284127768	24.55067304451884	25.51252108625282	24.718423027950653
35	25.843602869187443	24.371360579740934	25.243434884194226	24.54160166687739
36	24.578075185269963	25.0031608815875	24.98182199324958	25.43694193989296
37	25.586267156301716	24.718094014253815	24.65830752534228	25.03733130410219
38	24.73543773627296	24.571682919159922	25.22989832066709	25.462981023900028
39	25.41405198698422	24.25089456474073	24.66831894211756	25.66673450615749
40	25.58998031088034	24.57633611287238	25.229381299143483	24.604302277103795
41	24.50409410543761	24.752029426985047	25.75570921017442	24.988167257402928
42	25.81121852102703	25.093028622781567	24.983373057820398	24.112379798371006
43	24.775060385763858	24.066552890596835	25.555856890322342	25.60252983331696
44	24.418221530562317	24.65482938054711	25.242682852887167	25.684266236003406
45	24.551002058215683	24.907229887980236	25.325923318187737	25.215844735616344
46	24.48067464823686	24.31983372564355	25.399799207357887	25.799692418761705
47	24.640505365275878	24.24394967028112	25.790737790060962	25.32480717438204
48	25.183695397239386	25.05251293611353	24.84443527384045	24.919356392806634
49	24.34804760922197	24.97698079171036	25.31703994837305	25.35793165069462
50	24.139005597468284	25.34300885188682	25.032137618098403	25.48584793254649
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	16.0
2	22.0
3	58.0
4	94.0
5	142.5
6	191.0
7	209.0
8	227.0
9	323.5
10	420.0
11	603.0
12	786.0
13	1368.5
14	1951.0
15	2791.5
16	3632.0
17	4391.5
18	5151.0
19	5659.0
20	6167.0
21	7313.5
22	8460.0
23	9432.0
24	10404.0
25	12405.5
26	14407.0
27	18497.5
28	22588.0
29	25909.0
30	29230.0
31	31956.5
32	34683.0
33	39980.5
34	45278.0
35	52378.5
36	59479.0
37	69775.0
38	80071.0
39	85338.5
40	90606.0
41	98909.5
42	107213.0
43	109766.0
44	112319.0
45	123243.0
46	134167.0
47	142774.5
48	151382.0
49	156460.5
50	161539.0
51	156632.5
52	151726.0
53	148514.0
54	145302.0
55	146483.5
56	147665.0
57	144768.5
58	141872.0
59	129829.0
60	117786.0
61	104110.5
62	90435.0
63	79701.0
64	68967.0
65	59551.5
66	50136.0
67	44899.5
68	39663.0
69	36694.0
70	33725.0
71	26638.5
72	19552.0
73	17320.0
74	15088.0
75	11419.0
76	7750.0
77	7035.0
78	6320.0
79	5359.5
80	4399.0
81	3541.5
82	2684.0
83	2309.0
84	1934.0
85	1596.5
86	1259.0
87	904.5
88	550.0
89	366.0
90	182.0
91	117.0
92	52.0
93	39.0
94	26.0
95	17.0
96	8.0
97	8.0
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014805616357808974
2	0.0046061917557627926
3	4.2301761022311355E-4
4	9.400391338291413E-5
5	0.0
6	2.3500978345728532E-4
7	4.7001956691457065E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.410058700743712E-4
47	4.7001956691457065E-5
48	0.0
49	0.0
50	9.400391338291413E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2127571.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.05493795027707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58346032809658	43.01879009903598
2	12.656980027301085	13.683445400273127
3	3.459317401331125	5.609795624378032
4	1.393390176394219	3.012784781020605
5	0.7014475714729059	1.8958352475670237
6	0.4234633786119008	1.3734171993048594
7	0.29783702483327684	1.126969332766054
8	0.21510391022111475	0.9301942815891464
9	0.1634722651694171	0.7952834835291673
>10	0.9180153381688717	9.31666879671874
>50	0.0918394868637411	3.4948197836805575
>100	0.08229577403660059	9.37475188548807
>500	0.009792546143224824	3.5749994585219422
>1k	0.0035847713560019433	2.7922446261267053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2268	0.10660043777622463	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2247	0.10561339668570403	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2236	0.105096375162098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7001956691457065E-5
2	0.0	0.0	0.0	0.0	4.7001956691457065E-5
3	0.0	0.0	0.0	0.0	4.7001956691457065E-5
4	0.0	0.0	0.0	0.0	4.7001956691457065E-5
5	0.0	0.0	0.0	0.0	4.7001956691457065E-5
6	0.0	0.0	0.0	0.0	1.410058700743712E-4
7	0.0	0.0	0.0	0.0	1.410058700743712E-4
8	0.0	0.0	0.0	0.0	1.410058700743712E-4
9	0.0	0.0	0.0	0.0	1.410058700743712E-4
10	0.0	0.0	0.0	0.0	1.410058700743712E-4
11	0.0	0.0	0.0	0.0	1.410058700743712E-4
12	0.0	0.0	0.0	0.0	2.820117401487424E-4
13	0.0	0.0	0.0	0.0	3.760156535316565E-4
14	0.0	0.0	0.0	4.7001956691457065E-5	3.760156535316565E-4
15	0.0	0.0	0.0	4.7001956691457065E-5	4.230176102231136E-4
16	0.0	0.0	0.0	9.400391338291413E-5	4.230176102231136E-4
17	0.0	0.0	0.0	9.400391338291413E-5	5.170215236060277E-4
18	0.0	0.0	0.0	1.8800782676582826E-4	5.170215236060277E-4
19	0.0	0.0	0.0	2.3500978345728532E-4	5.170215236060277E-4
20	0.0	0.0	0.0	4.230176102231136E-4	6.110254369889418E-4
21	0.0	0.0	0.0	5.640234802974848E-4	6.110254369889418E-4
22	0.0	0.0	0.0	8.930371771376842E-4	6.580273936803989E-4
23	0.0	0.0	0.0	0.001457060657435169	6.580273936803989E-4
24	0.0	0.0	0.0	0.00272611348810451	7.05029350371856E-4
25	0.0	0.0	0.0	0.003478144795167823	7.05029350371856E-4
26	0.0	0.0	0.0	0.00432418001561405	7.05029350371856E-4
27	0.0	0.0	0.0	0.00625126023996379	7.05029350371856E-4
28	0.0	0.0	0.0	0.013724571353905464	7.05029350371856E-4
29	0.0	0.0	0.0	0.028483185755022983	7.05029350371856E-4
30	0.0	0.0	0.0	0.05668435976989722	7.05029350371856E-4
31	0.0	0.0	0.0	0.1137447351933261	7.05029350371856E-4
32	0.0	0.0	0.0	0.18213258217939612	7.05029350371856E-4
33	0.0	0.0	0.0	0.25094344677568925	7.05029350371856E-4
34	0.0	0.0	0.0	0.3310347809779321	7.52031307063313E-4
35	0.0	0.0	0.0	0.415967316719395	7.52031307063313E-4
36	0.0	0.0	0.0	0.5420735665225743	7.52031307063313E-4
37	0.0	0.0	0.0	0.7288593424144247	7.52031307063313E-4
38	0.0	0.0	0.0	0.9727054937297039	7.52031307063313E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCTA	70	1.1329957E-6	25.142735	41
GTATCAA	4095	0.0	19.021353	1
TATACGG	60	0.007414114	18.333244	5
GCATATA	265	0.0	18.267069	1
GCGCAAA	540	0.0	17.111029	22
TACCGTT	90	2.2164488E-4	17.111029	7
CGCGCAA	545	0.0	16.550379	21
TAGTACG	175	3.6288839E-9	16.343546	2
CCTTATA	285	0.0	16.21121	2
AACGCAG	4790	0.0	16.121008	6
GTCCTAA	550	0.0	16.002556	1
CGACGGT	725	0.0	15.779235	7
TAGGACG	575	0.0	15.686881	4
GTAGAAC	830	0.0	15.638479	3
CGCAAAT	585	0.0	15.41873	23
TATACCG	100	4.9460714E-4	15.399926	5
GTGTACG	115	8.211017E-5	15.306792	1
TATGGCG	710	0.0	15.183025	16
ATACCGT	610	0.0	15.147467	6
GACGGTA	730	0.0	15.068421	8
>>END_MODULE